FastQCFastQC Report
Mon 6 Feb 2017
SRR2077483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131878
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.038.870391
GTATCAA54450.032.843641
ATCAACG68750.025.2415313
TCAACGC68850.025.1555654
TATCAAC70750.024.5256332
CAACGCA71050.024.3766465
AACGCAG74500.023.366536
GTGGTAT12700.021.2036321
GTACATG84650.020.3921241
ACGCAGA86850.019.806957
TACATGG86250.019.5981752
CGCAGAG89400.019.166098
AGAGTAC87100.018.24870911
ACATGGG88350.018.2360483
CAGAGTA92250.017.71525810
GAGTACT49950.017.257112
GTGCCGA4650.017.05392311
GCAGAGT98600.016.958739
TGGTATC14400.016.7692742
ATACCGT2702.9594958E-916.5973476
Produced by FastQC (version 0.11.5)