FastQCFastQC Report
Mon 6 Feb 2017
SRR2077483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131878
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34950.039.9904331
GTATCAA65950.033.572761
ATCAACG90650.024.3381183
TCAACGC91000.024.2828984
TATCAAC91350.024.147752
CAACGCA93500.023.705485
AACGCAG96100.022.9922776
GTGGTAT15100.022.2237191
GCACCGT7800.020.3605086
CGTGCCG4300.020.07453710
ACGCAGA112950.019.3801067
CGCAGAG115600.018.9059728
GCGCGTT1651.8362553E-618.8119136
GTACATG78200.018.1826421
GAGTACT81150.018.1257112
AGAGTAC114750.018.08405111
TGGTATC17750.017.9206622
AGTACTT83950.017.71915813
GTGCCGA5150.017.43171311
TACATGG79350.017.3420622
Produced by FastQC (version 0.11.5)