FastQCFastQC Report
Mon 6 Feb 2017
SRR2077482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389216
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51200.034.0623171
GGTATCA25050.033.2247771
TCAACGC62000.027.0473444
ATCAACG62600.026.6778643
CAACGCA63650.026.3465865
TATCAAC65100.026.2352372
AACGCAG67300.024.995096
ACGCAGA78650.021.1701557
CGCAGAG82050.020.2106678
GTACATG86200.019.630961
AGAGTAC79750.019.32544311
TACATGG86400.019.0087682
GAGTACT43200.018.51696212
ACATGGG90000.018.2875673
CAGAGTA89900.017.48767710
GTATAGG8300.017.4752711
GCAGAGT94200.017.091759
AGTACTT47550.016.96951113
GTACTTT47100.016.76607514
GTATAGC9100.016.6979851
Produced by FastQC (version 0.11.5)