FastQCFastQC Report
Mon 6 Feb 2017
SRR2077482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389216
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.037.996311
GTATCAA63400.031.3273371
TCAACGC84000.023.3421634
ATCAACG84650.023.1625843
CAACGCA84700.023.1595865
TATCAAC85100.023.1186352
AACGCAG90200.021.8548496
ATGCGCG1605.7510988E-821.55379951
GTGGTAT15550.020.2493881
GTACATG81900.020.06831
TACATGG83350.019.0823442
TACCGTA1108.199094E-418.83254212
ACGCAGA105750.018.543547
CCGTATA1702.417435E-618.2786439
CGAATCG2851.6370905E-1118.15137137
ATACCGT2902.1827873E-1117.8571246
TGCGCGA1954.6198147E-717.68490652
CGCAGAG110150.017.6782198
CGTGCCG4500.017.64678810
ACATGGG88350.017.5348643
Produced by FastQC (version 0.11.5)