FastQCFastQC Report
Mon 6 Feb 2017
SRR2077481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755310
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.038.9791681
GTATCAA48800.034.732511
ATCAACG65650.025.6968543
TATCAAC66700.025.5500142
TCAACGC66200.025.3786744
CAACGCA67250.024.8294335
AACGCAG70800.023.661686
GTACATG86900.020.7757361
GTGGTAT12900.020.3375381
ACGCAGA81850.020.3014097
TACATGG89300.019.8178232
CGCAGAG84850.019.6673838
ACTCGAC3900.019.46625546
AGAGTAC82800.018.90645811
ACATGGG91700.018.3969293
CAGAGTA88050.018.05262810
GAGTACT42550.017.90957512
GCAGAGT91250.017.7593049
TGGTATC13100.017.6438562
GTGTAGC12350.017.050511
Produced by FastQC (version 0.11.5)