FastQCFastQC Report
Mon 6 Feb 2017
SRR2077481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755310
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.044.9185751
GTATCAA60050.034.2840731
TATCAAC85450.024.0386372
ATCAACG85300.024.0028863
TCAACGC86500.023.6299134
GTGGTAT11150.023.2742021
CAACGCA88350.023.1767085
AACGCAG92450.022.1452376
TACCGTA1356.7950677E-620.4562327
TGTACCG3150.019.7317475
GTACATG81500.019.3171121
TGGTATC12450.019.165282
ACGCAGA108300.018.7421027
TACATGG84700.018.1682052
CGCAGAG111850.018.1170448
TATAGCG1351.567292E-417.9047345
GAGTACT72100.017.85874412
AGAGTAC110100.017.84030511
TAATTCG2751.9826984E-1017.55813259
ACATGGG86700.017.3524553
Produced by FastQC (version 0.11.5)