FastQCFastQC Report
Mon 6 Feb 2017
SRR2077480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731336
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.032.7254031
GTATCAA45450.032.432561
ATCAACG58400.024.3994143
TCAACGC58550.024.3968144
TATCAAC59650.024.00252
CAACGCA59700.023.637255
AACGCAG62350.022.7237596
GTACATG93650.021.0850931
TATACCG2001.2805685E-920.701385
TACATGG96150.020.1294732
ACATGGG99100.018.8001923
ACGCAGA74600.018.577677
CGCAGAG78700.017.6124368
CGCACGA4650.017.06175635
AGAGTAC77550.017.02929711
CGTGCCG4050.017.02719310
CGAATCG3551.8189894E-1216.52400237
CATGGGG72300.016.4164714
GTGTAGC17850.016.2453211
GCACCGT8400.016.0051656
Produced by FastQC (version 0.11.5)