FastQCFastQC Report
Mon 6 Feb 2017
SRR2077480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731336
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.035.429221
GTATCAA54500.032.4941751
CGTACCG500.00770432327.62402710
CGCGTAA700.001327658724.6643110
TCAACGC73050.023.5725694
ATCAACG73750.023.4429153
TATCAAC74250.023.3259752
CAACGCA73450.023.3208925
AACGCAG77900.022.2076766
GTACATG87800.019.9337061
CGAATCG3150.019.70764537
TACATGG90650.018.5720442
GCGATAC3650.017.95670514
ACGCAGA95900.017.8936887
ACATGGG91950.017.5241833
CGCAGAG99600.017.298998
GAGTACT62600.016.7685812
AGAGTAC99550.016.71871211
TGCGATA4000.016.39340613
GTGGTAT16950.016.1209051
Produced by FastQC (version 0.11.5)