FastQCFastQC Report
Mon 6 Feb 2017
SRR2077479_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077479_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161238
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.037.8607861
GTATCAA72100.034.4751781
TATCAAC91650.026.9880962
TCAACGC91900.026.917244
ATCAACG92000.026.8875563
CAACGCA93600.026.3186135
AACGCAG98800.024.9489616
GTACATG116150.021.9651661
GAGTACT62750.021.59591912
ACGCAGA115150.021.2288727
TACATGG117350.021.224612
AGAGTAC111550.020.61779411
CGCAGAG119050.020.5054448
AGTACTT66500.020.37939513
ACTCGAC5100.020.29995346
GCGATAC2402.910383E-1120.11579314
ACATGGG121800.019.9409283
GTACTTT69100.019.26325214
GTGGTAT17800.019.0070781
GTATAGG8750.018.9383641
Produced by FastQC (version 0.11.5)