FastQCFastQC Report
Mon 6 Feb 2017
SRR2077479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161238
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.10986202241020765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.043.8322221
GTATCAA86650.035.2470321
TATCAAC121450.025.1069982
TCAACGC122700.024.7146554
ATCAACG123600.024.5898673
CAACGCA124300.024.3285715
AACGCAG127350.023.7459096
CGATAGC1403.698733E-722.2286974
GTACATG108200.021.0982841
CCGATAG1154.5275836E-521.0467953
TACATGG111000.020.3694152
ACGCAGA151000.019.867057
GAGTACT101500.019.72879812
CGCAGAG153600.019.4870438
ACATGGG114900.019.048883
AGTACTT105700.019.03442213
AGAGTAC150450.018.9321711
GTGGTAT18600.018.7818721
CGAATCG3001.8189894E-1218.39606337
TGGTATC18400.018.2254242
Produced by FastQC (version 0.11.5)