FastQCFastQC Report
Mon 6 Feb 2017
SRR2077478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672937
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48550.037.1892621
GGTATCA22850.031.274421
TCAACGC62050.028.1879124
TATCAAC64050.027.7363512
ATCAACG63600.027.6625373
CAACGCA63400.027.5892055
AACGCAG65900.026.4710736
ACGCAGA78550.022.210497
CGCAGAG81100.021.51368
GCGATAC3350.020.58949314
GTACATG92850.020.2265621
AGAGTAC79900.020.15474111
TATACCG2402.910383E-1120.1250655
GAGTACT41850.019.61076512
TACATGG92650.019.5095422
CAGAGTA88350.018.69494610
TGCGATA3750.018.39302813
ACATGGG96250.018.3505123
GTGCGAT4550.018.1891712
AGTACTT45850.017.97682813
Produced by FastQC (version 0.11.5)