FastQCFastQC Report
Mon 6 Feb 2017
SRR2077478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672937
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.036.2144741
GTATCAA56650.034.750441
TCAACGC76550.025.7040464
ATCAACG77550.025.3725973
TATCAAC78250.025.2309062
CAACGCA77450.025.1882425
AACGCAG80400.024.2154886
GTACATG86100.019.9710641
ACGCAGA98550.019.6514327
TACATGG87050.018.9862922
CGCAGAG102650.018.8000328
GAGTACT63900.018.1538112
AGAGTAC100100.018.14182911
ACATGGG89650.017.6664643
AGTACTT69350.017.06889513
CAGAGTA108850.016.93722310
GTACTTT70400.016.46443614
GCAGAGT117550.016.1823129
TATACTG13250.016.1564755
TACTTTT76000.015.2964515
Produced by FastQC (version 0.11.5)