FastQCFastQC Report
Mon 6 Feb 2017
SRR2077477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597065
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.037.3076741
GTATCAA43300.036.1126981
ATCAACG55450.027.8075123
TCAACGC55500.027.7213764
CAACGCA56350.027.2446295
TATCAAC57350.026.8229962
AACGCAG59750.025.5004186
ACGCAGA68300.022.007077
GTACATG64500.021.5137421
TACATGG64000.021.0179582
CGCAGAG73400.020.575878
AGAGTAC68950.020.2054811
GAGTACT43600.020.01080112
ACATGGG66950.019.2697983
AGTACTT46800.018.79113
GTACTTT46800.018.6436214
CGGCACG2801.2732926E-1118.47982222
CGAATCG2255.5315468E-918.40301737
CAGAGTA77000.018.31666210
TCGCACG950.00785056818.1524618
Produced by FastQC (version 0.11.5)