FastQCFastQC Report
Mon 6 Feb 2017
SRR2077477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597065
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.10049806223563908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.041.0161061
GTATCAA59150.037.31471
TATCAAC81050.027.0046312
TCAACGC81400.026.8916324
ATCAACG81500.026.8167253
CAACGCA82300.026.639415
AACGCAG85900.025.5631686
ACGCAGA100600.021.5896347
CGCAGAG103950.020.8282328
AGAGTAC102350.020.37864111
GTACATG61050.020.3433671
GTGGTAT11800.020.2293131
GAGTACT77200.019.7711112
TACATGG61600.019.5899032
CAGAGTA108550.019.34193210
AGTACTT79050.019.16959413
GCAGAGT111700.018.8884989
GTACTTT80850.018.86035314
ACATGGG65700.017.4224363
CGTACAC2601.717126E-917.2908463
Produced by FastQC (version 0.11.5)