FastQCFastQC Report
Mon 6 Feb 2017
SRR2077476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265410
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48350.023.1623951
GGTATCA23450.018.0144311
TATCAAC63100.017.398832
TCAACGC63500.017.1149434
ATCAACG63550.017.1012153
GTATACG1053.774571E-516.763431
TGCGATA2109.094947E-1216.76317213
CAACGCA64800.016.7031755
CGATTAG800.00198872916.5007414
AACGCAG66050.016.3525056
GTATAGC5200.016.0783841
GTACATG80700.014.5862091
AGAGTAC67200.014.47133211
GCGATAC2601.6370905E-1114.38526314
ACGCAGA75500.014.24797
CGTCGTA1901.7540697E-713.89578710
TACATGG81900.013.8616412
CGCAGAG78700.013.6126668
GAGTACT31350.013.54488312
GTATAGG5150.013.2439221
Produced by FastQC (version 0.11.5)