FastQCFastQC Report
Mon 6 Feb 2017
SRR2077476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265410
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55000.024.3225231
GGTATCA25450.021.8726041
ATCAACG80200.016.7069573
TATCAAC80200.016.6244032
TCAACGC80850.016.5459334
CAACGCA81150.016.513655
AACGCAG84200.015.9394066
TTATACT7150.015.0784874
CGTGCCG2407.2759576E-1114.66841310
GAGTACT55150.014.28319112
GTACATG83400.014.0614121
TATACTG7850.013.7353825
ACTAGAC4050.013.5812413
ACGCAGA98750.013.5683797
TACATGG84350.013.5633212
AGTACTT58450.013.32620313
TATACCG1505.2692398E-513.2027855
AGAGTAC93500.013.20177411
TAGCGAT1000.00876133313.2007627
CGCAGAG101900.013.1059638
Produced by FastQC (version 0.11.5)