FastQCFastQC Report
Mon 6 Feb 2017
SRR2077475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3450955
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49470.1433516229565439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67950.021.0771871
GGTATCA35550.020.9169431
ATCAACG86350.016.2794883
TCAACGC86300.016.1881284
TATCAAC86900.016.1516062
CAACGCA87150.015.9283385
AACGCAG89950.015.5289936
GTACATG90650.015.1196361
GGACCGT3100.014.90144356
TATAACG1351.9583882E-514.6664022
TACATGG91050.014.4489412
TGGACCG4150.014.3127855
GTATAGA6650.014.22551251
ACATGGG93500.013.9287563
AGAGTAC94250.013.562345511
CCGATCG1302.332258E-413.5386099
TACCGAC1302.3334047E-413.5378237
GAGTACT54800.013.48978512
ACGCAGA102800.013.4390557
CGCAGAG105750.013.0225548
Produced by FastQC (version 0.11.5)