FastQCFastQC Report
Mon 6 Feb 2017
SRR2077475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3450955
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59770.17319843347710995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38550.11170820830755544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.023.1893731
GTATCAA83700.022.636091
TATCAAC112150.016.7949712
ATCAACG114200.016.5320223
TCAACGC115350.016.4053574
CAACGCA115650.016.3437735
AACGCAG119350.015.8898736
ACGTTCG1552.680208E-715.60838944
CGTACAC2153.5415724E-914.328293
ACGCAGA138350.013.5957647
AGAGTAC130000.013.55598311
GTCCTAC3900.013.5416111
GTACATG86250.013.4964721
GAGTACT93050.013.47761112
CGCAGAG142300.013.171998
TACATGG86050.013.1181122
AGTACTT97150.012.88617113
CCGTACA1557.166955E-512.7766092
GCGACTA1902.6544149E-612.73777315
TCCTATA5250.012.5738062
Produced by FastQC (version 0.11.5)