FastQCFastQC Report
Mon 6 Feb 2017
SRR2077474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616841
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48800.1864843909125545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.11116456827143872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.030.176371
GTATCAA57650.027.1698781
TATCAAC78650.019.6915972
ATCAACG79400.019.2832033
CAACGCA80350.019.0836855
TCAACGC80250.019.0537284
AACGCAG82600.018.6167626
GTACATG105250.016.4915661
TACATGG108200.015.9402752
ACGCAGA96400.015.7928697
CGCAGAG97900.015.5508938
GAGTACT50400.015.49665812
AACGTAG1158.224557E-515.3040895
ACATGGG112300.015.0638853
AGAGTAC96450.014.91789211
AGTACTT52950.014.83346113
CGCCTAA2102.5447662E-914.66782122
ATATCGT1655.422553E-714.66697911
GTGTAAG5250.014.6661391
GTACTTT52450.014.63957614
Produced by FastQC (version 0.11.5)