##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077474_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2616841
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.746537141538212	31.0	30.0	34.0	16.0	34.0
2	28.70055727497391	31.0	30.0	34.0	16.0	34.0
3	28.78649447941239	31.0	30.0	34.0	16.0	34.0
4	32.10243075525032	35.0	33.0	37.0	19.0	37.0
5	32.18786812037873	35.0	33.0	37.0	19.0	37.0
6	32.25925037096255	37.0	33.0	37.0	19.0	37.0
7	32.04658670511506	36.0	33.0	37.0	17.0	37.0
8	31.971479352394738	36.0	33.0	37.0	17.0	37.0
9	33.13499253489226	38.0	33.0	39.0	16.0	39.0
10	33.01976505259586	38.0	33.0	39.0	15.0	39.0
11	33.08226063410043	38.0	33.0	39.0	15.0	39.0
12	33.00067485949662	38.0	33.0	39.0	13.0	39.0
13	33.0040640604454	38.0	33.0	39.0	13.0	39.0
14	33.82323114014187	39.0	33.0	41.0	11.0	41.0
15	33.92420594143855	39.0	33.0	41.0	12.0	41.0
16	33.830545302523156	39.0	33.0	41.0	11.0	41.0
17	33.67125706147221	39.0	33.0	41.0	10.0	41.0
18	33.48520601748444	39.0	32.0	41.0	10.0	41.0
19	33.84224949089379	39.0	33.0	41.0	11.0	41.0
20	33.88688384200645	39.0	33.0	41.0	11.0	41.0
21	33.889065480096036	39.0	33.0	41.0	11.0	41.0
22	33.803122543555375	39.0	33.0	41.0	10.0	41.0
23	33.54867834920043	39.0	33.0	41.0	10.0	41.0
24	33.70482386969633	39.0	33.0	41.0	10.0	41.0
25	33.61124806589319	39.0	33.0	41.0	10.0	41.0
26	33.64132631673075	39.0	33.0	41.0	10.0	41.0
27	33.63004095395937	39.0	33.0	41.0	10.0	41.0
28	33.51010359437199	39.0	33.0	41.0	9.0	41.0
29	33.47283919810183	39.0	33.0	41.0	8.0	41.0
30	33.240913758229865	38.0	32.0	41.0	8.0	41.0
31	33.156168448904616	38.0	32.0	41.0	8.0	41.0
32	33.15294356821832	38.0	32.0	41.0	5.0	41.0
33	33.132382517699774	38.0	32.0	41.0	2.0	41.0
34	33.10941360212561	38.0	32.0	41.0	2.0	41.0
35	33.037440180737	38.0	32.0	41.0	2.0	41.0
36	32.90628547932412	38.0	32.0	40.0	2.0	41.0
37	32.80558505465177	38.0	32.0	40.0	2.0	41.0
38	32.68840177909166	38.0	32.0	40.0	2.0	41.0
39	32.61620289501731	38.0	32.0	40.0	2.0	41.0
40	32.55733955559394	38.0	32.0	40.0	2.0	41.0
41	32.432718686385606	38.0	31.0	40.0	2.0	41.0
42	32.24316838508721	38.0	31.0	40.0	2.0	41.0
43	32.12571073290276	38.0	31.0	40.0	2.0	41.0
44	32.0379866411448	38.0	31.0	40.0	2.0	41.0
45	31.937134506834767	38.0	31.0	40.0	2.0	41.0
46	31.743158640513503	38.0	31.0	40.0	2.0	41.0
47	31.537161027360852	38.0	30.0	40.0	2.0	41.0
48	31.334382562792314	38.0	30.0	40.0	2.0	41.0
49	31.18640337720175	38.0	30.0	40.0	2.0	41.0
50	30.979116041058667	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6839240089320917
1101	2	0.6924018439539914
1101	3	0.8001006749312474
1101	4	0.7265637660026485
1101	5	0.5851434973976453
1101	6	0.626733212292983
1101	7	0.7618486621595508
1101	8	0.7723103119088961
1101	9	1.0073279620824138
1101	10	0.9870220637691887
1101	11	0.9073889855290531
1101	12	0.9789905076285592
1101	13	0.948702266441984
1101	14	1.2096005879958156
1101	15	1.1921894641209576
1101	16	1.0270461113824894
1101	17	1.263187596212255
1101	18	1.3698832845508164
1101	19	0.9312617025127707
1101	20	1.00567776771193
1101	21	1.0645889594818883
1101	22	0.9595450325863268
1101	23	1.1739170079572787
1101	24	1.1426819631902063
1101	25	1.0536294528722294
1101	26	0.9791712062260345
1101	27	1.059026740276515
1101	28	1.1887684039038575
1101	29	1.117461799221438
1101	30	1.258804548463118
1101	31	1.2453460448773725
1101	32	1.2643998314230558
1101	33	1.2344660459848953
1101	34	1.1233861467641404
1101	35	1.1635130124894886
1101	36	1.1449231383154412
1101	37	1.2612224829235075
1101	38	1.1556830187539404
1101	39	1.2305789081740244
1101	40	1.0917652775359983
1101	41	1.1789591734531015
1101	42	1.295921279185876
1101	43	1.3107488123954454
1101	44	1.0777882169605704
1101	45	1.1732185250613796
1101	46	1.0964975086849513
1101	47	1.1709453037245012
1101	48	1.1970199589250328
1101	49	1.1841921382958631
1101	50	1.1988934167332097
1102	1	0.6579200359677415
1102	2	0.5282987165746746
1102	3	0.5141133969298686
1102	4	0.5706067836746129
1102	5	0.5058333225149667
1102	6	0.7224207342222684
1102	7	0.5988696165502674
1102	8	0.634787304530569
1102	9	0.8045003332347918
1102	10	0.8877788815557963
1102	11	0.625082398019245
1102	12	0.5871567583212709
1102	13	0.6620361430196695
1102	14	1.0391651636477732
1102	15	0.8940925974927652
1102	16	1.003260269506356
1102	17	1.0463985175518786
1102	18	1.2643762709582305
1102	19	1.0036842250875182
1102	20	0.960820205540557
1102	21	0.8030532077031154
1102	22	1.077991342543072
1102	23	1.24479464440752
1102	24	1.108632583398844
1102	25	1.1061962544259103
1102	26	1.0423902874318287
1102	27	1.024177615193942
1102	28	1.0569353910566903
1102	29	0.9496017451266283
1102	30	1.1045956788510054
1102	31	1.1935397154086402
1102	32	1.1593990991632737
1102	33	1.0440956541461048
1102	34	0.8812239866453879
1102	35	0.950647964186949
1102	36	1.0385083606129868
1102	37	0.9856920286172155
1102	38	1.063706219768072
1102	39	1.0627661079370085
1102	40	1.0268696994610025
1102	41	1.0913553514770697
1102	42	1.0663772292096354
1102	43	1.0202554065137903
1102	44	0.9014716124729816
1102	45	0.8749159816417347
1102	46	0.7932137786012241
1102	47	0.9068568635442418
1102	48	0.7859833618972516
1102	49	0.9039918127412818
1102	50	0.9395382729792736
1103	1	0.5120771379879123
1103	2	0.526054808416994
1103	3	0.5391449228980534
1103	4	0.5010722245409909
1103	5	0.48637645219030645
1103	6	0.5181181671140962
1103	7	0.6358662017610186
1103	8	0.6919869656972821
1103	9	0.7541272269832575
1103	10	0.8048119338817941
1103	11	0.727611354116803
1103	12	0.7015483155532607
1103	13	0.6926968177767421
1103	14	0.9519225999521197
1103	15	0.8742276813960572
1103	16	0.9889753690346907
1103	17	1.0551380361252072
1103	18	0.7354695127155892
1103	19	0.6786287647403455
1103	20	0.784334361973265
1103	21	0.8026168502338535
1103	22	0.8102693367472824
1103	23	1.0960570128225484
1103	24	0.9835339820722737
1103	25	0.7807412005852399
1103	26	0.8367487557112838
1103	27	0.8723057000608634
1103	28	0.9258246771339529
1103	29	0.9586278852754333
1103	30	1.1125008269689403
1103	31	1.0860039181192889
1103	32	1.0105547489720408
1103	33	1.064808546805125
1103	34	0.9430093181489099
1103	35	1.0114269935204092
1103	36	1.0464154328268478
1103	37	1.0056518438737712
1103	38	0.8731496001187509
1103	39	0.9127789134825832
1103	40	0.9382845978319736
1103	41	0.9440177058324579
1103	42	1.0131042355245867
1103	43	1.067497665102799
1103	44	1.0368477721960332
1103	45	0.9817306552645562
1103	46	0.9622732990656679
1103	47	0.761822292007075
1103	48	0.9851294893552733
1103	49	0.9129410577707091
1103	50	0.9134339948138255
1104	1	1.0814524255615403
1104	2	1.1050886172947223
1104	3	0.9909979622883718
1104	4	1.0605728667496876
1104	5	0.9784960972912842
1104	6	1.0795434255719485
1104	7	0.9912196470036534
1104	8	1.0220356733343507
1104	9	1.38820634687999
1104	10	1.4269010589960516
1104	11	1.4265241415597956
1104	12	1.3326982375851344
1104	13	1.2263869813584094
1104	14	1.5997088491022993
1104	15	1.4722986971094727
1104	16	1.5466901404778
1104	17	1.6570365111441845
1104	18	1.8194142149695267
1104	19	1.5831805143269264
1104	20	1.6293718694799892
1104	21	1.5623094047130621
1104	22	1.5714149336181578
1104	23	1.727047287105819
1104	24	1.5314072034034112
1104	25	1.6706020075788146
1104	26	1.4647857141315868
1104	27	1.4309971164385402
1104	28	1.4425153367424457
1104	29	1.5934795180505077
1104	30	1.7912233662832833
1104	31	1.7952568282907322
1104	32	1.6550808477277386
1104	33	1.6854810075081943
1104	34	1.4712096224108535
1104	35	1.566261473039269
1104	36	1.5502885823399453
1104	37	1.6414467583526928
1104	38	1.691019120677062
1104	39	1.5365702333078772
1104	40	1.6556788062431025
1104	41	1.689092408651824
1104	42	1.8065491914194638
1104	43	1.7556215340691779
1104	44	1.8418431053633952
1104	45	1.63221711528643
1104	46	1.834067246680842
1104	47	1.8181136326006424
1104	48	1.8399958180305234
1104	49	1.921354286439076
1104	50	1.8631409343390182
1105	1	0.3834193778892754
1105	2	0.45798813170963726
1105	3	0.46062803243782824
1105	4	0.2957454623007223
1105	5	0.3745154509782722
1105	6	0.30261468520944135
1105	7	0.3993693850919229
1105	8	0.5471061027132507
1105	9	0.658855068271194
1105	10	0.618871687277867
1105	11	0.563625873589082
1105	12	0.6025927546038474
1105	13	0.5667143102812986
1105	14	0.7604948694911684
1105	15	0.682572513436412
1105	16	0.611002436016058
1105	17	0.6793909069197994
1105	18	0.9253115333187694
1105	19	0.6204330872307082
1105	20	0.620774309341698
1105	21	0.6416809644478434
1105	22	0.7249026535020064
1105	23	0.966055075269459
1105	24	0.803699012891812
1105	25	0.7997785199223131
1105	26	0.7524528881785102
1105	27	0.6530640906318652
1105	28	0.8039852113480421
1105	29	0.7658730500614155
1105	30	0.975513838484936
1105	31	0.985000757949031
1105	32	0.7849429043818006
1105	33	0.8900945939612512
1105	34	0.7441241258454383
1105	35	0.7967432659753158
1105	36	0.7506753497502885
1105	37	0.6864349435404975
1105	38	0.7562673056747329
1105	39	0.7491486510209029
1105	40	0.8421605907286747
1105	41	0.773523158641531
1105	42	0.9383559122888485
1105	43	0.7480900534307864
1105	44	0.825638802595762
1105	45	0.7770618537602587
1105	46	0.7552491926760077
1105	47	0.9419349241914787
1105	48	1.0700661787493644
1105	49	0.9833619805912477
1105	50	0.8248114547717229
1106	1	0.40715949045959476
1106	2	0.44695227710208485
1106	3	0.49880686389233375
1106	4	0.5544770130907679
1106	5	0.582547691183116
1106	6	0.5597270545272721
1106	7	0.5422666802273639
1106	8	0.5086918287270308
1106	9	-0.7911930740718134
1106	10	-0.22491784766144463
1106	11	0.3332531958209728
1106	12	0.6251100310992328
1106	13	0.6676746896213714
1106	14	0.8145093959869314
1106	15	0.939931120685884
1106	16	0.9564775955437952
1106	17	0.9540778017669496
1106	18	-0.7817456509427743
1106	19	0.2713903050608053
1106	20	0.4454534160789052
1106	21	0.6422813943103876
1106	22	0.7583704495388588
1106	23	-0.827918444128585
1106	24	0.38508758919960684
1106	25	0.5373224276411719
1106	26	0.7333936096093794
1106	27	0.8525136023456454
1106	28	0.7424477047872671
1106	29	0.6807858926290109
1106	30	-0.1520420367058435
1106	31	0.6216489302107107
1106	32	0.6770843930057637
1106	33	0.839441549375735
1106	34	0.8232883357018466
1106	35	0.7350045023159169
1106	36	0.8110761264022059
1106	37	0.7419459180926538
1106	38	0.7249283882065995
1106	39	0.7781218337052849
1106	40	0.9069780996335908
1106	41	0.9780480505204565
1106	42	1.1530747460977793
1106	43	0.9570648348624928
1106	44	0.910443480396772
1106	45	1.014705747445099
1106	46	0.9456201586021216
1106	47	0.9991849862650284
1106	48	1.1154387305711424
1106	49	1.1825371092235741
1106	50	1.112216101831546
1107	1	0.22239286843082695
1107	2	0.30536725541587373
1107	3	0.3038035141728628
1107	4	0.2658714648942251
1107	5	0.26226358191694743
1107	6	0.36268534357515136
1107	7	0.36197431741257446
1107	8	0.20407131803307976
1107	9	0.434433869034514
1107	10	0.5488375695118961
1107	11	0.38928685768625115
1107	12	0.36177615804020746
1107	13	0.3433435136058094
1107	14	0.6286137295504233
1107	15	0.552309516609462
1107	16	0.5263873796026317
1107	17	0.7286381776762951
1107	18	0.760409886655296
1107	19	0.6530031856018255
1107	20	0.6250058875512323
1107	21	0.6880248981444836
1107	22	0.7128915948150834
1107	23	0.8870474568033018
1107	24	0.6981950152161076
1107	25	0.6579720154517474
1107	26	0.6681552890188271
1107	27	0.6131753327328937
1107	28	0.582358016893032
1107	29	0.6745458044576864
1107	30	0.7860380797747979
1107	31	0.7848439594622576
1107	32	0.7880947469798869
1107	33	0.6380185505046541
1107	34	0.6377670570374718
1107	35	0.6580445410360198
1107	36	0.7860131674424622
1107	37	0.7857922068862777
1107	38	0.7930249191885608
1107	39	0.7885302392112905
1107	40	0.8080721578043466
1107	41	0.5985753867089159
1107	42	0.8051189759088047
1107	43	0.791186526404509
1107	44	0.7508838415007872
1107	45	0.7593234852670534
1107	46	0.8603377022980077
1107	47	0.9293671724643815
1107	48	0.8095546611462368
1107	49	0.9834534082803259
1107	50	1.0234409029192726
1108	1	0.16777869868715456
1108	2	0.17667530563842604
1108	3	0.16800407855044597
1108	4	0.11227298027600341
1108	5	0.1444813110570422
1108	6	0.14791876027231154
1108	7	0.08485142729355744
1108	8	0.16398263707419858
1108	9	0.3105729281701457
1108	10	0.15416292553056365
1108	11	0.22022659135158307
1108	12	0.2303313781681382
1108	13	0.21161642336011255
1108	14	0.13725799537931493
1108	15	0.17235963217191141
1108	16	0.40678601838516926
1108	17	0.07225573830555021
1108	18	0.5205350661387911
1108	19	0.2893113148518296
1108	20	0.2855235295179668
1108	21	0.4148248091474116
1108	22	0.3378618379399967
1108	23	0.1470447333957381
1108	24	0.2045094301880397
1108	25	0.357724355576174
1108	26	0.36388938717578867
1108	27	0.27845212942200703
1108	28	0.2735084012703979
1108	29	0.30073635301762636
1108	30	0.3459976663518063
1108	31	0.3894309559206164
1108	32	0.337537238703284
1108	33	0.2915895578858141
1108	34	0.2553211209329902
1108	35	0.3700807866727942
1108	36	0.3233154620349197
1108	37	0.22134981850646795
1108	38	0.29132291104022556
1108	39	0.3411824612808587
1108	40	0.39980223893514477
1108	41	0.29145610753653983
1108	42	0.2952942831128311
1108	43	0.3423192329488316
1108	44	0.2068225113350337
1108	45	0.24532358982644809
1108	46	0.3627315046005002
1108	47	0.43717581417701723
1108	48	0.3844748738151864
1108	49	0.22384263671707672
1108	50	0.4324980567881518
1109	1	-0.10340305345545531
1109	2	-0.11249718465619907
1109	3	0.002409808843356842
1109	4	-0.015976924164768036
1109	5	-0.045069732667116114
1109	6	-0.13497447727434064
1109	7	-0.17620388474279736
1109	8	-0.10191561061411747
1109	9	-0.10903715394738356
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2211	4	0.14097828844376892
2211	5	-0.10302049433227012
2211	6	-0.1256091496656424
2211	7	0.07040790145783404
2211	8	0.017677295060892106
2211	9	0.0888396839163974
2211	10	-0.13158951196535895
2211	11	0.049841263393581414
2211	12	0.06703058003622431
2211	13	0.026182169204780337
2211	14	0.1403238372959592
2211	15	-0.0438399836576977
2211	16	-0.15675075837939545
2211	17	0.10823096289950485
2211	18	0.305466808026587
2211	19	0.08831827727916419
2211	20	0.09998321424910728
2211	21	0.12479426002114025
2211	22	-0.0024206430556716896
2211	23	0.22141296465479599
2211	24	0.1740980364627447
2211	25	0.21168578223623769
2211	26	0.08224477593236657
2211	27	0.06933516311065802
2211	28	0.08612162371257881
2211	29	0.10464104726488443
2211	30	0.15571245303382142
2211	31	0.20939459546795547
2211	32	0.03670067799784249
2211	33	0.08736844308656089
2211	34	0.06127123754145458
2211	35	-0.09076186152924492
2211	36	0.07870909566516104
2211	37	0.05806788221247672
2211	38	0.17670457533593975
2211	39	0.07652419841500091
2211	40	0.061145049019941666
2211	41	0.11082010516499707
2211	42	0.21710479626646162
2211	43	0.16785451839425036
2211	44	0.22985769248212762
2211	45	0.2630906772707533
2211	46	0.08085843595575071
2211	47	0.01811595634003993
2211	48	-0.07839333599661202
2211	49	-0.013738018205891933
2211	50	-0.019838842666501932
2212	1	-0.27627516724188084
2212	2	-0.28348403281791335
2212	3	-0.5170226542431706
2212	4	-0.4174612883170603
2212	5	-0.41679069942683356
2212	6	-0.2522093249146167
2212	7	-0.5102488607469233
2212	8	-0.3944003207018376
2212	9	-0.5774000058212323
2212	10	-0.2960126624487813
2212	11	-0.3479481100702344
2212	12	-0.43973914125418645
2212	13	-0.32528505203852376
2212	14	-0.4137781460514063
2212	15	-0.5224162096797755
2212	16	-0.624092610921906
2212	17	-0.37602233838470056
2212	18	-0.2544045650992359
2212	19	-0.38102619989641084
2212	20	-0.5962594066081621
2212	21	-0.5812755472293603
2212	22	-0.4191132713224732
2212	23	-0.18576478917550077
2212	24	-0.2233820566221496
2212	25	-0.33975197920391054
2212	26	-0.3831803466815771
2212	27	-0.43930599276932725
2212	28	-0.5955169357696235
2212	29	-0.6022997738991904
2212	30	-0.3590968342701757
2212	31	-0.38774200201870457
2212	32	-0.380080401009657
2212	33	-0.377875973713067
2212	34	-0.4821289194942793
2212	35	-0.4878074284619416
2212	36	-0.5811832429016803
2212	37	-1.684515793644568
2212	38	-1.0608226367900393
2212	39	-0.7619189781958653
2212	40	-0.6102336406405939
2212	41	-1.4365960602659413
2212	42	-0.958800663206901
2212	43	-0.7380368656416856
2212	44	-0.588957087803081
2212	45	-0.5375201916014412
2212	46	-0.5094277467724204
2212	47	-0.3133083151733018
2212	48	-0.40840049349236907
2212	49	-0.3904970986582015
2212	50	-0.2946945856310279
2213	1	-0.8699806497605032
2213	2	-0.8735451479817975
2213	3	-0.7942121707383158
2213	4	-0.8396999129753979
2213	5	-0.8591234389328406
2213	6	-0.9757790516183711
2213	7	-0.9088358856720156
2213	8	-0.9594932927710822
2213	9	-0.9861115779399441
2213	10	-1.1155076796217642
2213	11	-1.0229763664408367
2213	12	-0.9266652864590554
2213	13	-1.0151882133080434
2213	14	-2.6821338287910663
2213	15	-1.6408558005337923
2213	16	-1.4792994538164805
2213	17	-1.117136062057746
2213	18	-1.0660605919247814
2213	19	-1.1404306401384972
2213	20	-1.3646252647721369
2213	21	-1.4566547774663405
2213	22	-1.2252526527192913
2213	23	-1.723592320429706
2213	24	-1.4039692255686376
2213	25	-1.3634106234616823
2213	26	-1.4321170931131348
2213	27	-1.501312680371278
2213	28	-1.207781609514214
2213	29	-1.6262221964642123
2213	30	-1.3464573918208096
2213	31	-1.262877022072118
2213	32	-1.2361803330691288
2213	33	-1.2903383637793482
2213	34	-1.398151142620982
2213	35	-1.4167391341448337
2213	36	-1.2833110023984844
2213	37	-1.532392417407472
2213	38	-1.305436706427514
2213	39	-1.372074990932365
2213	40	-1.6348973037307282
2213	41	-1.80721867099804
2213	42	-1.5133691156344433
2213	43	-1.632028512401046
2213	44	-1.6319349492106703
2213	45	-1.6404070377521585
2213	46	-1.7560635949488308
2213	47	-1.7049095199435378
2213	48	-1.6103991033728633
2213	49	-1.7237015432835427
2213	50	-1.7633174906677063
2214	1	-0.6153397165558872
2214	2	-0.6142747492472722
2214	3	-0.7990780557034078
2214	4	-0.8129077760471937
2214	5	-0.7212672199433428
2214	6	-0.6605912863994376
2214	7	-0.7726924623602969
2214	8	-0.6122092435652604
2214	9	-0.8362551902945441
2214	10	-1.110385857007465
2214	11	-0.901152130448601
2214	12	-0.8270149578696291
2214	13	-0.8922593738205009
2214	14	-1.3258366506035557
2214	15	-1.1216876980910797
2214	16	-0.8569861620401511
2214	17	-1.326512751078269
2214	18	-1.5186307526645138
2214	19	-0.9167839937151712
2214	20	-0.8917132445408456
2214	21	-1.0862235164460756
2214	22	-0.8802098968262442
2214	23	-0.7062109164573513
2214	24	-0.7475912639837219
2214	25	-0.8589428637287
2214	26	-1.0677651476279095
2214	27	-0.888438501149821
2214	28	-0.8280955874976641
2214	29	-0.7934406436205848
2214	30	-0.7548483936577242
2214	31	-0.6343744293351179
2214	32	-0.7309190449967389
2214	33	-0.7345196887984997
2214	34	-0.8559940591078146
2214	35	-0.9053964455056374
2214	36	-0.9447130796924625
2214	37	-1.0557427460663966
2214	38	-1.064867131350038
2214	39	-0.9848672168219252
2214	40	-0.9802657879195849
2214	41	-0.8941550550571833
2214	42	-0.8512648459758942
2214	43	-0.9116411760499759
2214	44	-0.9124384426813954
2214	45	-0.9011815552352651
2214	46	-0.8858761088924787
2214	47	-0.8940899541907896
2214	48	-0.960166251658702
2214	49	-0.9577974422345648
2214	50	-0.8539158855175835
2215	1	-0.33481697377404274
2215	2	-0.49773662106567684
2215	3	-0.45714918098545354
2215	4	-0.3082079344897508
2215	5	-0.44163375562036933
2215	6	-0.44156879984483055
2215	7	-0.46331147337545175
2215	8	-0.4452267049285439
2215	9	-0.6098944406468547
2215	10	-0.9300391397228438
2215	11	-0.7234243888825276
2215	12	-0.794469686700161
2215	13	-0.7794084340156004
2215	14	-0.7537565625973457
2215	15	-0.7515254578806605
2215	16	-0.7081009792952244
2215	17	-0.7440342166457725
2215	18	-0.858208317616814
2215	19	-0.7862842590933781
2215	20	-0.8728699209598361
2215	21	-0.8542355721990305
2215	22	-1.1927174136967125
2215	23	-1.1065471820238102
2215	24	-0.9269673245160988
2215	25	-0.8154728017896886
2215	26	-0.840405798079054
2215	27	-0.9714986125825291
2215	28	-0.9453146460978843
2215	29	-0.9615869898986276
2215	30	-0.8671979908841934
2215	31	-0.8209547565739044
2215	32	-0.8929679020402403
2215	33	-0.95319133359434
2215	34	-0.9352187690873421
2215	35	-0.9656593594891092
2215	36	-1.0133499507918629
2215	37	-0.9591004667421039
2215	38	-0.858069200515704
2215	39	-0.9985500454137508
2215	40	-0.939352605812612
2215	41	-1.0328846189741157
2215	42	-0.9676916917924636
2215	43	-1.1340049115293453
2215	44	-0.9664216650265018
2215	45	-0.9991263774841563
2215	46	-1.0107785529865723
2215	47	-1.0812773504252924
2215	48	-1.1254452386721638
2215	49	-1.2405473030090484
2215	50	-1.2091896564765818
2216	1	-0.9148028786929672
2216	2	-1.1784000773905525
2216	3	-0.9378989374803517
2216	4	-2.3093998520832066
2216	5	-1.3994202758650296
2216	6	-1.2548818124629335
2216	7	-1.2155039393154077
2216	8	-1.1807373848045977
2216	9	-1.0211405120570234
2216	10	-1.3602979298275848
2216	11	-1.2120902078815696
2216	12	-1.3929793066850031
2216	13	-1.4085806822853044
2216	14	-1.2117778475191656
2216	15	-1.3565781273589863
2216	16	-1.4924997547773913
2216	17	-1.3693652793093918
2216	18	-1.214647647431896
2216	19	-1.5528508862594705
2216	20	-1.5665339121372241
2216	21	-1.5654142963549802
2216	22	-1.8851258508723987
2216	23	-3.5505249083608774
2216	24	-2.3067678098088855
2216	25	-1.8584244638806666
2216	26	-2.1908091762369537
2216	27	-2.059638258156866
2216	28	-2.275761186608051
2216	29	-2.0890813837610693
2216	30	-1.8841441363886133
2216	31	-1.7672629555194064
2216	32	-1.8621671137461533
2216	33	-1.901754714843591
2216	34	-1.977611810852455
2216	35	-2.189514302826641
2216	36	-1.9527552095206318
2216	37	-1.9085171892247992
2216	38	-2.210475975477735
2216	39	-2.213256373356849
2216	40	-2.3045499921503207
2216	41	-2.2363958459699376
2216	42	-2.59318299553226
2216	43	-2.4774866418998904
2216	44	-2.4889489535450835
2216	45	-2.3936604547041007
2216	46	-2.5359189211024926
2216	47	-2.5063020076167284
2216	48	-2.6167103366016953
2216	49	-2.9132287603103997
2216	50	-2.9816022461195786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155409.0
3	7684.0
4	15159.0
5	11638.0
6	9438.0
7	7799.0
8	7116.0
9	6312.0
10	6306.0
11	5759.0
12	6020.0
13	6135.0
14	6442.0
15	6803.0
16	7425.0
17	7986.0
18	8655.0
19	10195.0
20	13452.0
21	18337.0
22	23600.0
23	26685.0
24	27474.0
25	27623.0
26	29576.0
27	33289.0
28	38215.0
29	42599.0
30	48712.0
31	58259.0
32	69981.0
33	87398.0
34	109774.0
35	139704.0
36	181155.0
37	244697.0
38	385707.0
39	718323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.058651188988094	18.04839922805346	12.93809629601022	23.95485328694822
2	15.569964364201757	20.783368074317025	39.27819057790871	24.368476983572503
3	18.286695370873133	26.74462178511613	29.135088215725112	25.83359462828562
4	12.284927340912203	16.737825329067384	37.737445187305866	33.239802142714545
5	13.37580105037228	38.81615884394797	33.76565958820389	14.042380517475864
6	31.053583302838522	37.56410124047921	17.883457434787946	13.49885802189432
7	27.717475456077857	33.06539145448282	22.03805067790888	17.179082411530434
8	26.654211725335657	34.53038167028258	20.197805676914335	18.61760092746743
9	27.54572320337147	15.972924537644385	18.89833777950773	37.58301447947642
10	15.005143226428153	29.208937359708457	33.11866895594415	22.66725045791923
11	34.18323259697914	24.110731480518528	23.627288744241817	18.078747178260524
12	23.563059273422564	25.72868068833652	29.88818355640535	20.820076481835564
13	27.75270578662066	22.855866769452906	25.945533998996613	23.445893444929826
14	22.585775508182817	21.663385025083624	27.02082902335058	28.73001044338298
15	23.490562249379963	29.381990350949323	24.02670985729119	23.100737542379527
16	23.321299777047777	28.606147056686464	25.250811691415763	22.821741474849993
17	22.159769141183016	28.370620958966718	26.65084516280786	22.8187647370424
18	22.182922734632474	27.606481470858853	27.934516830454896	22.27607896405378
19	23.286421349738955	27.301313670656533	27.870444762009285	21.541820217595227
20	23.59432302202049	26.703320953332714	27.667064035237683	22.035291989409114
21	23.97525552400348	26.58577861916645	27.437914120987866	22.0010517358422
22	23.662164446405885	26.127116654135712	27.597253847054027	22.613465052404376
23	23.460180432921227	26.361057816777716	27.5046052805177	22.674156469783355
24	22.757565340814583	26.615438912317718	27.566170563491276	23.060825183376423
25	23.177872112589874	26.39578552852991	27.68081688848095	22.745525470399265
26	22.634209179595018	26.87870667781459	27.888254062476815	22.59883008011358
27	22.8047336056091	26.84928698755637	27.35308256885646	22.992896837978073
28	23.113006363964285	26.53521027628946	27.577879859279005	22.773903500467245
29	23.01549842032389	26.719169541702687	27.56840800777216	22.696924030201266
30	23.164868768818266	26.604005716661188	27.508085664252093	22.72303985026845
31	23.188818152718994	26.467667171087328	27.477464557660557	22.866050118533117
32	22.792218048836645	26.829175012056755	27.38437850061785	22.994228438488754
33	23.020695415526035	26.684098330185847	27.283918888840446	23.011287365447675
34	23.46145992580226	26.49739558438559	27.31038164190987	22.73076284790228
35	23.24704321925384	26.725611052722563	27.37200286996284	22.655342858060756
36	23.297660756085246	26.78120649297003	27.04064225938222	22.880490491562504
37	23.293668264668227	26.308463390106823	27.516546449976993	22.881321895247957
38	23.261945473744035	26.63932717780522	27.306190929475054	22.79253641897569
39	23.496429912416808	26.713294093086105	27.00901067623278	22.781265318264303
40	23.285412040650186	26.567390297687627	27.508583503078743	22.638614158583444
41	23.00994282987529	26.70669187168687	27.282369638962557	23.000995659475283
42	23.497825234922427	26.630710550717435	26.995612232217113	22.87585198214303
43	23.083954337330468	26.410787406180386	27.22480503985219	23.280453216636957
44	22.977180743148317	26.74770444568561	27.240972896804326	23.034141914361747
45	23.30190807155173	26.74366238105615	26.811750448249626	23.142679099142498
46	23.313510116263245	26.538809720053763	26.962850200398503	23.184829963284486
47	23.28074344741591	26.84683541352179	26.781125132787135	23.09129600627516
48	23.2696244063608	27.019596688542713	26.6144959983117	23.096282906784786
49	23.258540979419674	26.640063804048776	26.737215103146312	23.36418011338524
50	23.4088966300829	26.896601027934054	26.607427672253888	23.087074669729162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2983.0
1	2648.5
2	2314.0
3	2300.0
4	2286.0
5	2185.0
6	2084.0
7	1944.0
8	1804.0
9	1958.0
10	2112.0
11	2746.5
12	3381.0
13	5543.5
14	7706.0
15	10927.5
16	14149.0
17	15210.0
18	16271.0
19	16738.0
20	17205.0
21	19064.5
22	20924.0
23	25619.0
24	30314.0
25	36568.5
26	42823.0
27	48385.5
28	53948.0
29	59754.0
30	65560.0
31	71367.0
32	77174.0
33	85651.5
34	94129.0
35	103839.0
36	113549.0
37	117547.5
38	121546.0
39	129160.5
40	136775.0
41	143057.0
42	149339.0
43	150583.5
44	151828.0
45	155979.5
46	160131.0
47	164375.5
48	168620.0
49	169562.0
50	170504.0
51	169401.0
52	168298.0
53	163505.0
54	158712.0
55	152762.5
56	146813.0
57	139608.5
58	132404.0
59	120802.5
60	109201.0
61	97638.0
62	86075.0
63	75589.0
64	65103.0
65	55891.0
66	46679.0
67	38737.5
68	30796.0
69	24429.5
70	18063.0
71	14290.5
72	10518.0
73	8592.5
74	6667.0
75	5042.0
76	3417.0
77	2675.0
78	1933.0
79	1514.0
80	1095.0
81	838.0
82	581.0
83	501.0
84	421.0
85	326.0
86	231.0
87	191.0
88	151.0
89	117.0
90	83.0
91	65.5
92	48.0
93	41.5
94	35.0
95	24.0
96	13.0
97	12.0
98	11.0
99	22.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06244169974408075
2	0.0677916617784573
3	0.0634734781364248
4	0.07745980745486639
5	0.08212191722768024
6	0.06274741186033084
7	0.046773953786263665
8	0.05911708047986103
9	0.05670959756439157
10	0.0669127394442383
11	0.05476068282329725
12	0.07035200075205181
13	0.06515489477580029
14	0.06832665798189497
15	0.07933229416689817
16	0.07379126205986532
17	0.08621081678252518
18	0.07180413330423972
19	0.06886165418533262
20	0.0817779910968989
21	0.08086085474814864
22	0.0757783908154909
23	0.0729123397256463
24	0.060836711133767776
25	0.07799480365830404
26	0.08800687546549446
27	0.07986729037033584
28	0.09897429763596642
29	0.09098756859893284
30	0.07749802146939765
31	0.07489946848127188
32	0.08116656686439871
33	0.07868265591986674
34	0.06370276222361236
35	0.08303905357643052
36	0.08869472772705717
37	0.09683431282221579
38	0.07887372599252306
39	0.057282807782360494
40	0.06305312397658093
41	0.056900667637047875
42	0.06175384748251804
43	0.040124715257824226
44	0.039131150880011435
45	0.04245577014423116
46	0.040239357301418005
47	0.031029779799384066
48	0.045665747364857096
49	0.05093928137017114
50	0.04237934211516863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2616841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.425499291954466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79457561529495	35.25113669916591
2	12.309729616751406	11.922096056802923
3	5.418657487943736	7.872035830372895
4	2.858534167607079	5.53703777237937
5	1.750159249384358	4.23761677459349
6	1.085477822324369	3.153888330984061
7	0.7481773391158141	2.5361602843926465
8	0.5280454276191637	2.045669078503328
9	0.39638497143714674	1.727562613830385
>10	1.9412503197654256	16.570023003177052
>50	0.11648209260142622	3.8430614165586094
>100	0.05052813275443747	4.340699596744017
>500	0.0016781162165711838	0.5003129937300057
>1k	3.196411841061731E-4	0.4626995487654182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4880	0.1864843909125545	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2909	0.11116456827143872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.821401453126117E-5
2	0.0	0.0	0.0	0.0	3.821401453126117E-5
3	0.0	0.0	0.0	0.0	3.821401453126117E-5
4	0.0	0.0	0.0	0.0	3.821401453126117E-5
5	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
6	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
7	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
8	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
9	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
10	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
11	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
12	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
13	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
14	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
15	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
16	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
17	0.0	3.821401453126117E-5	0.0	0.0	3.821401453126117E-5
18	0.0	3.821401453126117E-5	0.0	3.821401453126117E-5	3.821401453126117E-5
19	0.0	3.821401453126117E-5	0.0	3.821401453126117E-5	3.821401453126117E-5
20	0.0	3.821401453126117E-5	0.0	3.821401453126117E-5	3.821401453126117E-5
21	0.0	3.821401453126117E-5	0.0	1.146420435937835E-4	3.821401453126117E-5
22	0.0	3.821401453126117E-5	0.0	1.5285605812504467E-4	3.821401453126117E-5
23	0.0	3.821401453126117E-5	0.0	1.9107007265630583E-4	3.821401453126117E-5
24	0.0	3.821401453126117E-5	0.0	3.4392613078135047E-4	3.821401453126117E-5
25	0.0	3.821401453126117E-5	0.0	4.2035415984387285E-4	3.821401453126117E-5
26	0.0	3.821401453126117E-5	0.0	6.878522615627009E-4	3.821401453126117E-5
27	0.0	3.821401453126117E-5	0.0	9.17136348750268E-4	3.821401453126117E-5
28	0.0	3.821401453126117E-5	0.0	0.0015285605812504466	3.821401453126117E-5
29	0.0	3.821401453126117E-5	0.0	0.0024456969300007147	3.821401453126117E-5
30	3.821401453126117E-5	3.821401453126117E-5	0.0	0.003744973424063594	3.821401453126117E-5
31	3.821401453126117E-5	3.821401453126117E-5	0.0	0.007681016920783494	3.821401453126117E-5
32	3.821401453126117E-5	3.821401453126117E-5	0.0	0.011387776330315827	3.821401453126117E-5
33	3.821401453126117E-5	3.821401453126117E-5	0.0	0.015323819827035727	3.821401453126117E-5
34	3.821401453126117E-5	3.821401453126117E-5	0.0	0.019909501570787067	3.821401453126117E-5
35	3.821401453126117E-5	3.821401453126117E-5	0.0	0.027093736302664165	3.821401453126117E-5
36	3.821401453126117E-5	3.821401453126117E-5	0.0	0.03485118125251018	3.821401453126117E-5
37	3.821401453126117E-5	3.821401453126117E-5	0.0	0.04493968108876313	3.821401453126117E-5
38	3.821401453126117E-5	3.821401453126117E-5	0.0	0.05605995931736013	3.821401453126117E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2085	0.0	30.17637	1
GTATCAA	5765	0.0	27.169878	1
TATCAAC	7865	0.0	19.691597	2
ATCAACG	7940	0.0	19.283203	3
CAACGCA	8035	0.0	19.083685	5
TCAACGC	8025	0.0	19.053728	4
AACGCAG	8260	0.0	18.616762	6
GTACATG	10525	0.0	16.491566	1
TACATGG	10820	0.0	15.940275	2
ACGCAGA	9640	0.0	15.792869	7
CGCAGAG	9790	0.0	15.550893	8
GAGTACT	5040	0.0	15.496658	12
AACGTAG	115	8.224557E-5	15.304089	5
ACATGGG	11230	0.0	15.063885	3
AGAGTAC	9645	0.0	14.917892	11
AGTACTT	5295	0.0	14.833461	13
CGCCTAA	210	2.5447662E-9	14.667821	22
ATATCGT	165	5.422553E-7	14.666979	11
GTGTAAG	525	0.0	14.666139	1
GTACTTT	5245	0.0	14.639576	14
>>END_MODULE