FastQCFastQC Report
Mon 6 Feb 2017
SRR2077474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616841
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.23027765156537977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.14047471741691606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.1355833235569146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.030.8560351
GTATCAA69650.025.9648951
ACGCGTA600.007412234318.334246
GTCGCGC600.00741386918.3335430
TATCAAC99550.018.1217522
ATCAACG102250.017.7296353
GTGGTAT8100.017.6548581
CGCGATC750.001290821717.60120823
TCAACGC103800.017.4016324
CAACGCA105150.017.1795315
AACGCAG107550.016.7130536
CGAATCG1351.0884542E-616.29367637
CGGTTAC1653.1124728E-816.00109928
GTACCGA1958.931238E-1015.7956526
CCGTAAC850.002979817615.530478524
GTACATG97300.015.0364281
TACATGG99650.014.4828522
TAGACAG6300.014.3192645
GTATGCG1707.595845E-714.23599911
ACGCAGA124650.014.2084867
Produced by FastQC (version 0.11.5)