##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2616841
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40511976081084	31.0	30.0	34.0	26.0	34.0
2	30.344258210567627	31.0	30.0	34.0	26.0	34.0
3	30.50480483911709	31.0	31.0	34.0	26.0	34.0
4	33.965360142247846	37.0	35.0	37.0	30.0	37.0
5	33.57275012123396	37.0	35.0	37.0	28.0	37.0
6	33.511963088319085	37.0	35.0	37.0	28.0	37.0
7	33.32015013522029	37.0	35.0	37.0	27.0	37.0
8	33.27888282092798	37.0	35.0	37.0	26.0	37.0
9	34.69230839779719	39.0	35.0	39.0	27.0	39.0
10	34.54865121725011	39.0	35.0	39.0	27.0	39.0
11	34.48899073348362	39.0	35.0	39.0	26.0	39.0
12	34.41388567360416	39.0	35.0	39.0	25.0	39.0
13	34.38179125135994	39.0	35.0	39.0	25.0	39.0
14	35.510461277548	40.0	36.0	41.0	25.0	41.0
15	35.51659195189926	40.0	36.0	41.0	26.0	41.0
16	35.47062469595975	40.0	36.0	41.0	25.0	41.0
17	35.465763491171224	40.0	36.0	41.0	25.0	41.0
18	35.46751980727908	40.0	36.0	41.0	25.0	41.0
19	35.52268938005786	40.0	36.0	41.0	25.0	41.0
20	35.52557492029512	40.0	36.0	41.0	25.0	41.0
21	35.46582043005288	40.0	35.0	41.0	25.0	41.0
22	35.47450303629452	40.0	35.0	41.0	25.0	41.0
23	35.40451712580168	40.0	35.0	41.0	25.0	41.0
24	35.39284618362369	40.0	35.0	41.0	25.0	41.0
25	35.36750647058801	40.0	35.0	41.0	25.0	41.0
26	35.280743079155364	40.0	35.0	41.0	25.0	41.0
27	35.2190217135852	40.0	35.0	41.0	25.0	41.0
28	35.14660500962802	40.0	35.0	41.0	24.0	41.0
29	35.06289606437686	39.0	35.0	41.0	24.0	41.0
30	34.91634149724802	39.0	35.0	41.0	24.0	41.0
31	34.81279603919382	39.0	34.0	41.0	23.0	41.0
32	34.71378123470245	39.0	34.0	41.0	23.0	41.0
33	34.598687119316764	39.0	34.0	41.0	22.0	41.0
34	34.55387583731682	39.0	34.0	41.0	22.0	41.0
35	34.474825562577166	39.0	34.0	41.0	21.0	41.0
36	34.35629906440628	39.0	34.0	41.0	19.0	41.0
37	34.25122657433142	39.0	34.0	41.0	18.0	41.0
38	34.16205646426359	39.0	34.0	41.0	18.0	41.0
39	34.056613298247775	39.0	33.0	41.0	16.0	41.0
40	33.93735194457745	39.0	33.0	41.0	15.0	41.0
41	33.86560207517385	39.0	33.0	41.0	15.0	41.0
42	33.74107024461937	38.0	33.0	41.0	12.0	41.0
43	33.81470559349995	39.0	33.0	41.0	9.0	41.0
44	33.724428041291006	39.0	33.0	41.0	8.0	41.0
45	33.62606631430798	39.0	33.0	41.0	2.0	41.0
46	33.4341092943744	39.0	33.0	41.0	2.0	41.0
47	33.24580018426798	39.0	33.0	41.0	2.0	41.0
48	33.06265684464589	39.0	33.0	41.0	2.0	41.0
49	32.89629404308477	39.0	33.0	41.0	2.0	41.0
50	32.74517099051872	38.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7335132273824883
1101	2	0.8242745992515026
1101	3	0.7566854753620156
1101	4	0.8229018180955094
1101	5	0.825005234744637
1101	6	0.7853060174865973
1101	7	0.9203595968419904
1101	8	0.8991018317685331
1101	9	0.8961191976399618
1101	10	0.9353319806031237
1101	11	1.0209851657378977
1101	12	1.1322183094464293
1101	13	1.1081454550000132
1101	14	1.2216611630978207
1101	15	1.0557280753236782
1101	16	1.049586077502525
1101	17	1.1357533887393458
1101	18	1.0850211812007586
1101	19	1.1330425417573835
1101	20	1.078662575067419
1101	21	1.0386025052648478
1101	22	1.140506431507653
1101	23	1.0707570941750717
1101	24	1.1923336721907631
1101	25	1.1759342713970753
1101	26	1.0912291040846895
1101	27	1.1155884184350953
1101	28	1.2279975114035153
1101	29	1.2472314752552975
1101	30	1.3059206831964545
1101	31	1.3335391715040004
1101	32	1.2898426635056879
1101	33	1.4167126574542976
1101	34	1.3292070940988623
1101	35	1.1677576587078349
1101	36	1.2274568743383725
1101	37	1.1980264555645803
1101	38	1.2719523900182708
1101	39	1.167421419625974
1101	40	1.3130089703628087
1101	41	1.2667036335745863
1101	42	1.1870241034028979
1101	43	1.234757565637544
1101	44	1.311624734430957
1101	45	1.244129872220519
1101	46	1.241212251669161
1101	47	1.2059142558699136
1101	48	1.3313687051107053
1101	49	1.3054664561183955
1101	50	1.2376467678464564
1102	1	0.6918363048785352
1102	2	0.8021415923128146
1102	3	0.692494714828328
1102	4	0.6415228903198411
1102	5	0.6590410754306717
1102	6	0.7077695074059136
1102	7	0.7017819421622562
1102	8	0.6765164899012248
1102	9	0.7043213892668874
1102	10	0.7664107798762956
1102	11	0.8137518672114084
1102	12	0.8733721772481928
1102	13	0.7557352142279541
1102	14	0.9774899089566347
1102	15	0.971803263702057
1102	16	0.9640711560643425
1102	17	0.9794863510983944
1102	18	0.9049935037411743
1102	19	1.030689705671847
1102	20	0.9643953962949396
1102	21	0.9482798013193019
1102	22	0.845685062291075
1102	23	0.8959709546346346
1102	24	0.8703638065709356
1102	25	0.8171813028047197
1102	26	0.8213065976112617
1102	27	0.8409804994445906
1102	28	0.8514886432195183
1102	29	0.9451932168518837
1102	30	0.9008477330408056
1102	31	0.9949480589255657
1102	32	0.9326051086317051
1102	33	0.9251291645779389
1102	34	0.8841892328406686
1102	35	0.9968927492966344
1102	36	0.8429148503275314
1102	37	0.9019642350449004
1102	38	0.9532699927873622
1102	39	0.8064816825058401
1102	40	0.8210897300760251
1102	41	0.9702680725753865
1102	42	0.8108965563499524
1102	43	0.8530866160212156
1102	44	0.8810580129638197
1102	45	0.7868255878987469
1102	46	0.8058507687576935
1102	47	0.783028561481828
1102	48	0.7411686511782989
1102	49	0.7857100732072979
1102	50	0.7357995270377202
1103	1	0.5024052126290641
1103	2	0.6170266595348863
1103	3	0.6189249829462824
1103	4	0.5993842274606891
1103	5	0.5622795968756478
1103	6	0.7136736644770068
1103	7	0.7543031640813709
1103	8	0.6738716138958054
1103	9	0.7432776661797504
1103	10	0.8558863943311792
1103	11	0.9249049648443659
1103	12	0.8996942218420045
1103	13	0.8643406026998335
1103	14	0.9777493870359919
1103	15	0.9482042271518623
1103	16	1.093438501641387
1103	17	1.018946122684838
1103	18	0.9437287157755136
1103	19	0.9264521523907092
1103	20	0.8484598207988796
1103	21	0.9442179660987406
1103	22	0.9923084560403339
1103	23	1.0544074727659094
1103	24	1.0173345683528083
1103	25	0.9366598260803372
1103	26	1.005003196819814
1103	27	1.0075415126295226
1103	28	0.9468344681959167
1103	29	0.9196553787839363
1103	30	1.0020418475886927
1103	31	0.9113438919110166
1103	32	1.005680966319801
1103	33	1.0391088278460359
1103	34	1.0371248294330613
1103	35	1.01882455672569
1103	36	0.9648265990565505
1103	37	0.9711007811923764
1103	38	0.8468551188111775
1103	39	0.8674864495222181
1103	40	0.8310790176719536
1103	41	0.8676053230931586
1103	42	0.829826499261344
1103	43	0.8908397832377233
1103	44	0.9403972688018527
1103	45	0.9884267243247749
1103	46	0.937462709669532
1103	47	0.7418933833665449
1103	48	0.8183548587914089
1103	49	0.8696798871657734
1103	50	0.8037959089127611
1104	1	0.6724099665544685
1104	2	0.8243671464451126
1104	3	0.6511448195709661
1104	4	0.6597744218329922
1104	5	0.7405576950803834
1104	6	0.5938894830432062
1104	7	0.7323236284707377
1104	8	0.8096278055888675
1104	9	0.8199826143622317
1104	10	0.9261943784872599
1104	11	0.7865448332887226
1104	12	0.8290779292767496
1104	13	0.8106687446787362
1104	14	0.9785743565602232
1104	15	1.0036330143408065
1104	16	1.040326717131947
1104	17	1.0826424926313507
1104	18	0.9969645739863395
1104	19	0.9702711382396103
1104	20	0.9940637930867666
1104	21	1.0424280622679163
1104	22	1.0492860206745576
1104	23	1.126911400649881
1104	24	1.1638780036615088
1104	25	1.0475139837418368
1104	26	1.0099167029791474
1104	27	1.1915011412445153
1104	28	1.1843542751018603
1104	29	1.1359853805284672
1104	30	1.1606169482210404
1104	31	1.1541360271926209
1104	32	1.1871969057707616
1104	33	1.2536234812717382
1104	34	1.1307763559817516
1104	35	1.1879463239425263
1104	36	1.2007617864922508
1104	37	1.2672528483861925
1104	38	1.2898382954355867
1104	39	1.2160147022831254
1104	40	1.2306924731566937
1104	41	1.3228609430556801
1104	42	1.2634910173716776
1104	43	1.3006601765058008
1104	44	1.4749462340995407
1104	45	1.305151778878347
1104	46	1.2475060068356996
1104	47	1.3524888085038782
1104	48	1.3283659718289869
1104	49	1.4128042746691136
1104	50	1.451319673850179
1105	1	0.24363685848247485
1105	2	0.4456670293004841
1105	3	0.28422122343156175
1105	4	0.2849644253505019
1105	5	0.3007894703232594
1105	6	0.3360622359842296
1105	7	0.3131795659288201
1105	8	0.23251938712586195
1105	9	0.32977750034955733
1105	10	0.23289306538998744
1105	11	0.35201061786725063
1105	12	0.2567139210924694
1105	13	0.3749422208742885
1105	14	0.34688699856771166
1105	15	0.3715590473842525
1105	16	0.35992715782391116
1105	17	0.32428946794436087
1105	18	0.21598013658159942
1105	19	0.31420708157076405
1105	20	0.33439165016397965
1105	21	0.3486830995397696
1105	22	0.2573233675230071
1105	23	0.3718622453270868
1105	24	0.24194898337721327
1105	25	0.3208863969740037
1105	26	0.27748390797590616
1105	27	0.4052739799578049
1105	28	0.3324154733995144
1105	29	0.43383008836934067
1105	30	0.3690831563793324
1105	31	0.4076223738752347
1105	32	0.4010648122620992
1105	33	0.3443617142575093
1105	34	0.33195271410419025
1105	35	0.4438413184127512
1105	36	0.4723469254971846
1105	37	0.5112614657521206
1105	38	0.42973898129678645
1105	39	0.4830838577537975
1105	40	0.5542141956158204
1105	41	0.43150096680149375
1105	42	0.4888214571235352
1105	43	0.5046906805868545
1105	44	0.534291766389309
1105	45	0.4791279253165257
1105	46	0.535070983021221
1105	47	0.5109501576424051
1105	48	0.578206469223602
1105	49	0.6042132108289877
1105	50	0.6661398425849185
1106	1	0.42147680691130773
1106	2	0.5971249480604719
1106	3	0.5156153516593207
1106	4	0.49215295364447087
1106	5	0.5793534559437887
1106	6	0.556222465422465
1106	7	0.5718690917691518
1106	8	0.47717836301254124
1106	9	0.5575676357187334
1106	10	0.5504677708426371
1106	11	0.6900351522128148
1106	12	0.6090120304066886
1106	13	0.6820916543985334
1106	14	0.7063733020325742
1106	15	0.6679333866158146
1106	16	0.6637794962874608
1106	17	0.7657844406294316
1106	18	0.6993450678791646
1106	19	0.6044581157443716
1106	20	0.6389468462727024
1106	21	0.7321678340731737
1106	22	0.7522693211243805
1106	23	0.7096162520911733
1106	24	0.7298954052933126
1106	25	0.7504255927578356
1106	26	0.6584345508933822
1106	27	0.7158377157419338
1106	28	0.616620312514641
1106	29	0.5847258752331896
1106	30	0.7802774977391351
1106	31	0.6866555738425362
1106	32	0.7200443333884365
1106	33	0.7741479190729805
1106	34	0.6245562458449783
1106	35	0.6019130903439915
1106	36	0.7105541077281643
1106	37	0.709717415122725
1106	38	0.5995190070309633
1106	39	0.6836341147100171
1106	40	0.6838178553592087
1106	41	0.6579035077301967
1106	42	0.7240458960583211
1106	43	0.7476397422352719
1106	44	0.6685488860840749
1106	45	0.7356626122216525
1106	46	0.7198107342026745
1106	47	0.7089866286158895
1106	48	0.8111173511409433
1106	49	0.8238986997038111
1106	50	0.7847420901871871
1107	1	0.3391718095102476
1107	2	0.48571718246725837
1107	3	0.3678362894956848
1107	4	0.4835228900561148
1107	5	0.39302959600585297
1107	6	0.41578400628841905
1107	7	0.4287397205228842
1107	8	0.28523430695647534
1107	9	0.421802516997019
1107	10	0.44307516395004853
1107	11	0.4898850967929178
1107	12	0.5675365722008578
1107	13	0.5908103597976506
1107	14	0.6052738382629741
1107	15	0.3961561433360927
1107	16	0.5749255908549102
1107	17	0.6699362909083533
1107	18	0.5513964551034789
1107	19	0.5886481155366567
1107	20	0.5640511480532666
1107	21	0.5504640093367001
1107	22	0.5103327353056173
1107	23	0.47372805589196076
1107	24	0.5500239784629741
1107	25	0.5096962573486863
1107	26	0.610862277911643
1107	27	0.5544668258427166
1107	28	0.5335916906195166
1107	29	0.5506185485696022
1107	30	0.5763018549671344
1107	31	0.5887171341035753
1107	32	0.6337514555402066
1107	33	0.6245571053877086
1107	34	0.5338315277181778
1107	35	0.5661633125115983
1107	36	0.6098722014437286
1107	37	0.5819947513737418
1107	38	0.5582426300234786
1107	39	0.5907442790627471
1107	40	0.5147877824612053
1107	41	0.3641074584721835
1107	42	0.49736448578763515
1107	43	0.454633385901694
1107	44	0.5997035795578398
1107	45	0.6577476618117686
1107	46	0.6301622557911557
1107	47	0.5596635922810052
1107	48	0.5105711854498338
1107	49	0.5978481928913837
1107	50	0.6696827925196303
1108	1	0.2326852362742926
1108	2	0.4167382536174955
1108	3	0.30957105148323905
1108	4	0.30259318679811287
1108	5	0.3379630244963465
1108	6	0.2328226896181249
1108	7	0.2652814062878477
1108	8	0.2427453799182686
1108	9	0.3341085501958432
1108	10	0.29184798920576327
1108	11	0.37350902825058085
1108	12	0.32348606271695246
1108	13	0.3274692493525322
1108	14	0.3311891281295871
1108	15	0.3545357887824707
1108	16	0.37277008671447476
1108	17	0.3134100215755069
1108	18	0.26871282874041214
1108	19	0.3416821009883151
1108	20	0.335591417403144
1108	21	0.3870461814730817
1108	22	0.2606841643043154
1108	23	0.40773101516607824
1108	24	0.3422661574808217
1108	25	0.31329869333949034
1108	26	0.3566265888712863
1108	27	0.2252106200600963
1108	28	0.22808542252678876
1108	29	0.19348279521305756
1108	30	0.41120025605941635
1108	31	0.4200022785232065
1108	32	0.36680414010274376
1108	33	0.40717617240176907
1108	34	0.42545376677256996
1108	35	0.3080299895531553
1108	36	0.36058800778513245
1108	37	0.48949907660696823
1108	38	0.3512835942070254
1108	39	0.49774404441200915
1108	40	0.446178702085426
1108	41	0.446212725469465
1108	42	0.4192238436713538
1108	43	0.4228908549077346
1108	44	0.4234392923064263
1108	45	0.438885024896706
1108	46	0.42100431152618256
1108	47	0.37207260432843725
1108	48	0.34245183211323393
1108	49	0.38638690236093964
1108	50	0.3832488880407183
1109	1	0.12384517736270695
1109	2	0.2504799303030367
1109	3	0.18573166586416434
1109	4	0.16391213965012952
1109	5	0.19033974705333634
1109	6	0.2132815067609357
1109	7	0.13354082740121953
1109	8	0.10143782775017485
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1109	10	0.2667352655473536
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2211	7	-0.1750697647032311
2211	8	-0.21160744456850722
2211	9	-0.42217905228827846
2211	10	-0.40795810404927835
2211	11	-0.3188251917654128
2211	12	-0.19415163604480767
2211	13	-0.26046992738994135
2211	14	-0.38138766334838436
2211	15	-0.31577180019869644
2211	16	-0.26739322977247326
2211	17	-0.3158552657678513
2211	18	-0.3285690100126146
2211	19	-0.3228326121266605
2211	20	-0.2353276829400741
2211	21	-0.18107913399262543
2211	22	-0.20300932436595076
2211	23	-0.25341254104836963
2211	24	-0.26623342150650586
2211	25	-0.23683660068483192
2211	26	-0.2565887433937917
2211	27	-0.05855198167483877
2211	28	-0.10236745549000403
2211	29	-0.1545825736886428
2211	30	-0.21128070435482016
2211	31	-0.23402090449714308
2211	32	-0.19485033100485794
2211	33	-0.04788176752121842
2211	34	-0.1171991948003992
2211	35	-0.13890396287153095
2211	36	-0.1770093348698225
2211	37	-0.10396420950126384
2211	38	-0.1279480532540589
2211	39	-0.11602715385529194
2211	40	-0.09316826700525382
2211	41	-0.18244998197402396
2211	42	-0.2147371599194443
2211	43	-0.22131394822109485
2211	44	-0.2983837366462012
2211	45	-0.29497367819622156
2211	46	-0.23915784152030284
2211	47	-0.2757172994761916
2211	48	-0.18302457619326162
2211	49	-0.31980048559181284
2211	50	-0.40059350925775306
2212	1	-0.9690702201970005
2212	2	-2.313011194098287
2212	3	-1.229970381776699
2212	4	-0.7030197625054271
2212	5	-0.6900176018631967
2212	6	-0.6666225769448104
2212	7	-0.6594462312661449
2212	8	-0.6783060676318158
2212	9	-0.7640235105907962
2212	10	-0.7097267087085299
2212	11	-0.7890194023672166
2212	12	-0.772399185534276
2212	13	-2.3068717922739452
2212	14	-1.6357350796683647
2212	15	-1.2806046060077634
2212	16	-0.9887143105382847
2212	17	-0.8495928926951066
2212	18	-0.8179554263922171
2212	19	-0.7854367624280059
2212	20	-0.7695879895467215
2212	21	-0.9081935005031312
2212	22	-0.8182937787975604
2212	23	-0.7927655417129955
2212	24	-0.7658099219755385
2212	25	-0.8023339283490003
2212	26	-0.9178813142508346
2212	27	-0.9115703899502634
2212	28	-0.9198570201750442
2212	29	-0.8930618498524865
2212	30	-0.8523896246060758
2212	31	-0.807344755530643
2212	32	-0.8060904823150707
2212	33	-0.8184047616525021
2212	34	-0.8957656187209082
2212	35	-0.9262384825623116
2212	36	-0.8948237123118403
2212	37	-0.9835813842336165
2212	38	-1.0576865416249603
2212	39	-1.0520798838119063
2212	40	-1.0235203823897194
2212	41	-1.0274152118570825
2212	42	-0.9213966982172792
2212	43	-1.080301235983157
2212	44	-1.1241886220397745
2212	45	-1.144834549579869
2212	46	-1.1205927740822617
2212	47	-1.122176443347918
2212	48	-1.1814519600670401
2212	49	-1.1469154881392285
2212	50	-1.1192690925674995
2213	1	-1.0143907773719292
2213	2	-2.1726991373162896
2213	3	-1.2790801104216136
2213	4	-0.9183121728912482
2213	5	-0.8623298026665864
2213	6	-0.8757447665708824
2213	7	-0.8959238750761997
2213	8	-0.994847080853603
2213	9	-1.0573825590477384
2213	10	-1.0211862715722972
2213	11	-0.9961182480044641
2213	12	-0.9486453619229067
2213	13	-0.9728086983813924
2213	14	-1.053177948574465
2213	15	-1.0993388145319543
2213	16	-1.096047034389791
2213	17	-1.0730420797569806
2213	18	-1.082557160284523
2213	19	-1.0471909429619473
2213	20	-1.095123295138194
2213	21	-1.2281479772201962
2213	22	-1.203544736070171
2213	23	-1.0897055568039349
2213	24	-1.157779790195967
2213	25	-1.1363633650688172
2213	26	-1.12300299667114
2213	27	-1.2017140043235983
2213	28	-1.201225505163947
2213	29	-1.2705873118950208
2213	30	-1.0733732280838737
2213	31	-1.1598214538089593
2213	32	-1.0655943286254583
2213	33	-1.034678709122268
2213	34	-1.128952686852415
2213	35	-1.0397985660947242
2213	36	-1.0524911293508268
2213	37	-1.0020536929904296
2213	38	-1.0762144238530311
2213	39	-1.1461532470557714
2213	40	-1.1576825692606008
2213	41	-1.2223747762863084
2213	42	-1.0982494864400891
2213	43	-1.3132840129034165
2213	44	-1.0971118179370478
2213	45	-1.0289638620029677
2213	46	-1.145258785347238
2213	47	-1.0283112849400453
2213	48	-1.143669826859778
2213	49	-1.1300908153965281
2213	50	-1.2460564449847134
2214	1	-0.9282140398860008
2214	2	-1.7534866468639976
2214	3	-0.9683947396299892
2214	4	-0.6960737916728448
2214	5	-0.5469965485644579
2214	6	-0.5287424283235964
2214	7	-0.5490197070745353
2214	8	-0.5892710456527084
2214	9	-0.7350846038383878
2214	10	-0.6686796232168462
2214	11	-0.6531711227883434
2214	12	-0.5117175728280401
2214	13	-0.6656338839897629
2214	14	-0.7374091545035526
2214	15	-0.811937978063547
2214	16	-0.6571659759535322
2214	17	-0.6259931989672793
2214	18	-0.7503203219896264
2214	19	-0.7402887464736523
2214	20	-0.7328847030999128
2214	21	-0.7002273168489381
2214	22	-0.6641831588965417
2214	23	-0.6427361968090679
2214	24	-0.6705993763356943
2214	25	-0.7171415498911315
2214	26	-0.772645784395678
2214	27	-0.7567747123401531
2214	28	-0.7157093520753577
2214	29	-0.8364719989815654
2214	30	-0.6788802371498264
2214	31	-0.6449182259249469
2214	32	-0.7177531705154152
2214	33	-0.7732082543698837
2214	34	-0.7501706998588418
2214	35	-0.9042972746492097
2214	36	-0.7989275204759068
2214	37	-0.8599571564830342
2214	38	-0.912050664068154
2214	39	-0.8660133020403791
2214	40	-0.9152538408589237
2214	41	-0.8785948546325315
2214	42	-0.8626540442268578
2214	43	-0.8908575039022182
2214	44	-0.7515535379399836
2214	45	-0.8859005078494349
2214	46	-0.816386898734045
2214	47	-0.6985753843302618
2214	48	-0.7470426900786862
2214	49	-0.7617747611847747
2214	50	-0.9279580912562935
2215	1	-0.4978898732127952
2215	2	-0.30980385749062833
2215	3	-0.5545385654686754
2215	4	-0.5622473516365574
2215	5	-0.6323922823809625
2215	6	-0.5845185876864818
2215	7	-0.6331758109040848
2215	8	-0.6748153193661466
2215	9	-0.8001029188567017
2215	10	-0.8872403124681156
2215	11	-0.9240492305240551
2215	12	-0.8603333574716601
2215	13	-0.8884523901326773
2215	14	-1.0826848340940032
2215	15	-0.9985076836375342
2215	16	-1.1021790879902014
2215	17	-1.0960778520016135
2215	18	-0.9498051549059383
2215	19	-0.963877895250171
2215	20	-1.0977691197776878
2215	21	-0.940671740376871
2215	22	-0.9886359634082353
2215	23	-0.9963250449107619
2215	24	-1.0284154836164703
2215	25	-0.9854760140232415
2215	26	-1.042240141382969
2215	27	-1.1729142176124157
2215	28	-1.1509258575541708
2215	29	-1.115445776215509
2215	30	-1.0266119724618648
2215	31	-1.0838720867203548
2215	32	-1.0632354006336229
2215	33	-1.0808284981518028
2215	34	-1.0307615847056155
2215	35	-1.0623474834221227
2215	36	-1.1529484252195132
2215	37	-1.0777712244041666
2215	38	-1.1626688449593487
2215	39	-1.058322301629886
2215	40	-1.0261969662560162
2215	41	-1.217769940194131
2215	42	-1.1722108626166516
2215	43	-1.0087504662812634
2215	44	-0.9604620752746484
2215	45	-1.0088492192617409
2215	46	-1.01680153584568
2215	47	-1.0530394576112556
2215	48	-1.1521376248845385
2215	49	-0.9597408594453078
2215	50	-1.0069092525147347
2216	1	-1.2435549304150264
2216	2	-0.8939092614637474
2216	3	-1.0364960336535383
2216	4	-2.3320154268299476
2216	5	-1.6685406757380008
2216	6	-1.5286084061065068
2216	7	-1.3459534189847773
2216	8	-1.3430451415644882
2216	9	-1.3032917929430994
2216	10	-1.3182398917011113
2216	11	-1.5371955310866312
2216	12	-1.4205947359681375
2216	13	-1.5742171862876333
2216	14	-1.746137172110977
2216	15	-1.706626953293977
2216	16	-1.7631157177814814
2216	17	-1.696935732121048
2216	18	-1.6526815512267419
2216	19	-1.567310366440509
2216	20	-1.7481112843110793
2216	21	-1.6532486254252916
2216	22	-1.7296454423195868
2216	23	-1.6119858269730898
2216	24	-1.7558885017793742
2216	25	-1.7372751609580206
2216	26	-1.9843452705694773
2216	27	-2.113997944111418
2216	28	-2.053326774899837
2216	29	-1.9926525028445425
2216	30	-1.933795434233403
2216	31	-1.831446236169917
2216	32	-1.8248797899571798
2216	33	-1.918261089561284
2216	34	-1.8348370442984887
2216	35	-1.8957134783336613
2216	36	-2.0913037598826136
2216	37	-2.069780331617558
2216	38	-2.1462848466178883
2216	39	-2.1962799010744263
2216	40	-2.212581412352449
2216	41	-2.29518355415329
2216	42	-2.356998418218005
2216	43	-2.1170768072767245
2216	44	-2.1391853111814036
2216	45	-1.9870053704336321
2216	46	-1.9713355455957
2216	47	-2.0636305493728067
2216	48	-1.9626664296363856
2216	49	-1.9286336963471875
2216	50	-1.9571975637792676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71046.0
3	11553.0
4	19517.0
5	11465.0
6	8555.0
7	6652.0
8	5509.0
9	5005.0
10	4745.0
11	4694.0
12	4864.0
13	5297.0
14	5610.0
15	6089.0
16	6597.0
17	6968.0
18	7535.0
19	8604.0
20	9943.0
21	12471.0
22	15974.0
23	19549.0
24	22948.0
25	25361.0
26	27116.0
27	30687.0
28	36320.0
29	41718.0
30	46776.0
31	55430.0
32	68134.0
33	84448.0
34	107020.0
35	134980.0
36	173289.0
37	228681.0
38	357487.0
39	918204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81733473268059	18.515298162630547	14.180812380707048	25.486554723981808
2	14.53830433134416	20.68581399188026	38.66213039278028	26.113751283995306
3	17.177228129508087	26.17924393109971	29.85774746114089	26.785780478251315
4	11.74371920363377	16.752229643539824	37.2575747500942	34.246476402732206
5	13.035159354591826	38.60315241836921	34.115633528592994	14.246054698445965
6	31.19782397299231	37.59242610498505	17.617837550226593	13.591912371796047
7	27.36743886645495	32.73268819407387	22.31162244521948	17.588250494251696
8	26.855065057378386	33.92401811920898	20.280373010160808	18.94054381325182
9	27.165738238755754	14.978836583563673	19.236098714446207	38.61932646323437
10	14.628058199393665	28.91135880515634	33.571721544119534	22.888861451330463
11	34.34020732059765	23.299903817188433	23.89967016482584	18.46021869738808
12	23.486411227615186	25.18173922218365	30.42293171284438	20.90891783735678
13	28.029126373183182	22.069290583662823	26.462712225301242	23.43887081785275
14	22.381827662824822	20.79716150894808	27.86433444097997	28.95667638724713
15	23.4105523429284	28.88761122874674	24.59492800355366	23.1069084247712
16	23.10403701894147	27.933221242767825	25.81087890166538	23.151862836625327
17	21.823313092649776	27.717553577661562	27.375081670642036	23.084051659046622
18	21.780705336128865	26.679841443888296	29.150398913597016	22.389054306385827
19	23.023670684168106	26.447187519594305	28.98630348575982	21.542838310477773
20	23.203765312543727	25.82485684334315	28.875169842021116	22.09620800209201
21	23.51053218531997	25.835423636734134	28.731530874364257	21.922513303581635
22	23.161007537222563	25.353014602293474	28.94285818055309	22.54311967993088
23	22.86246454596857	25.57396440203065	28.82176203716695	22.741809014833827
24	22.432399679024872	25.777865790649745	28.826632525148337	22.963102005177046
25	22.69457996631259	25.684968426654187	28.95121747953027	22.66923412750295
26	22.331037620257327	25.985636979139787	29.132121059201104	22.55120434140178
27	22.516702628226792	26.135235855332073	28.535039200904063	22.813022315537076
28	22.64657162004749	25.818067268172378	28.845398810063124	22.68996230171701
29	22.656531937928545	25.925559211934747	28.747996807124636	22.66991204301207
30	22.693131939756245	25.929486762903558	28.7493128575721	22.628068439768096
31	22.751519145669786	25.66950974485692	28.772404913866655	22.806566195606635
32	22.501232784027707	26.058951923334213	28.567027136538954	22.872788156099126
33	22.720326635201808	26.015651397495347	28.48639962817037	22.77762233913247
34	23.030427656724907	25.825324580018222	28.514232291048746	22.63001547220812
35	22.86812135074021	26.07499797422604	28.529853792631076	22.527026882402676
36	23.000042809266372	26.044087428753148	28.07733646860246	22.878533293378016
37	22.921285014080546	25.642109543888186	28.610987105828826	22.825618336202442
38	22.837915262186062	25.964586258073812	28.420546498548156	22.776951981191967
39	23.257365840235124	25.982449636342093	28.0623129636495	22.697871559773283
40	23.022966251907995	25.8589891852931	28.47862625935271	22.6394183034462
41	22.593919993640704	26.074334968253204	28.225832956896664	23.10591208120943
42	23.143173249817035	25.99505857669429	27.915411087820136	22.946357085668534
43	22.773367309269425	25.826195355008537	28.09744439102797	23.302992944694076
44	22.777135541564363	26.04385252558064	28.034279490584606	23.144732442270396
45	23.03792592286871	26.13947253071452	27.672934343590676	23.14966720282609
46	23.13786064058343	25.97632530088253	27.660212732946547	23.22560132558749
47	23.09002175538812	26.296352485437495	27.416298312877867	23.19732744629652
48	23.107779671509697	26.53671249837589	27.166752011984013	23.188755818130403
49	23.12243320260269	26.207844526452035	27.240200074059047	23.429522196886236
50	23.27146314443124	26.43935031142725	27.13668444522232	23.152502098919186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1274.0
1	905.5
2	537.0
3	848.5
4	1160.0
5	1296.5
6	1433.0
7	1343.0
8	1253.0
9	1661.0
10	2069.0
11	2960.5
12	3852.0
13	7124.0
14	10396.0
15	14061.0
16	17726.0
17	19973.5
18	22221.0
19	22835.0
20	23449.0
21	24664.5
22	25880.0
23	29532.0
24	33184.0
25	38375.5
26	43567.0
27	48602.0
28	53637.0
29	58777.5
30	63918.0
31	69321.0
32	74724.0
33	83765.0
34	92806.0
35	102032.0
36	111258.0
37	116024.5
38	120791.0
39	127904.5
40	135018.0
41	140550.5
42	146083.0
43	148025.5
44	149968.0
45	154620.5
46	159273.0
47	163800.5
48	168328.0
49	169225.0
50	170122.0
51	169291.0
52	168460.0
53	163789.0
54	159118.0
55	153699.5
56	148281.0
57	140479.0
58	132677.0
59	121083.0
60	109489.0
61	97735.5
62	85982.0
63	75103.0
64	64224.0
65	54702.5
66	45181.0
67	37399.0
68	29617.0
69	23454.5
70	17292.0
71	13510.5
72	9729.0
73	8021.0
74	6313.0
75	4579.0
76	2845.0
77	2230.0
78	1615.0
79	1264.5
80	914.0
81	690.0
82	466.0
83	408.0
84	350.0
85	273.0
86	196.0
87	137.5
88	79.0
89	64.0
90	49.0
91	35.0
92	21.0
93	15.5
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014635967565473026
2	3.439261307813505E-4
3	6.114242325001787E-4
4	0.002101770799219364
5	0.03851972664751125
6	0.02663516812828903
7	0.029233721116414788
8	0.023386976893131833
9	0.029424791189071098
10	0.030571211625008934
11	0.03779366037141729
12	0.03840508460391747
13	0.035539033514072886
14	0.04146220576641837
15	0.036226885775635584
16	0.04199720196985602
17	0.04402254474001287
18	0.04727073597517006
19	0.04910500867267059
20	0.04490146707423187
21	0.04555110532126331
22	0.04417540079813791
23	0.043181836420325116
24	0.04058328343219936
25	0.039245792923605215
26	0.04188255992626224
27	0.04146220576641837
28	0.04096542357751197
29	0.03886365277829261
30	0.03573010358672919
31	0.03427797103454127
32	0.030991565784852806
33	0.02250805455891283
34	0.020520925803287245
35	0.02128520609391247
36	0.022355198500787783
37	0.017769516757036443
38	0.019565575440005718
39	0.014177399391097892
40	0.008368869182346195
41	0.007604588891720972
42	0.007107806702814577
43	0.003324619264219721
44	0.004432825685626295
45	0.003133549191563415
46	0.0014903465667191855
47	6.87852261562701E-4
48	8.024943051564845E-4
49	3.821401453126117E-4
50	6.87852261562701E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2616841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.488435311862396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.95208245056325	31.82010777482511
2	13.700199120945372	12.464012429455153
3	5.832414208233769	7.959221892696866
4	3.184869705318813	5.794989582684203
5	1.888134650288225	4.294414544986092
6	1.2259090199025944	3.3458809890040673
7	0.8093264769837667	2.5770496566117496
8	0.6050471206694366	2.201811744736021
9	0.42213496576043225	1.7282033174581803
>10	2.183697357429843	17.681385366070344
>50	0.13281768803164373	4.096810576170108
>100	0.060473024554139575	4.784052001095903
>500	0.002553715869307338	0.6983812092238917
>1k	2.5537158692864734E-4	0.32034307739347173
>5k	8.512386230954913E-5	0.233335837588911
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6026	0.23027765156537977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3676	0.14047471741691606	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3548	0.1355833235569146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	7.642802906252233E-5	0.0
19	0.0	0.0	0.0	7.642802906252233E-5	0.0
20	0.0	0.0	0.0	7.642802906252233E-5	0.0
21	0.0	0.0	0.0	1.5285605812504467E-4	0.0
22	0.0	0.0	0.0	1.9107007265630583E-4	0.0
23	0.0	0.0	0.0	1.9107007265630583E-4	0.0
24	0.0	0.0	0.0	3.4392613078135047E-4	0.0
25	0.0	0.0	0.0	4.2035415984387285E-4	0.0
26	0.0	0.0	0.0	7.260662760939622E-4	0.0
27	0.0	0.0	0.0	9.17136348750268E-4	0.0
28	0.0	0.0	0.0	0.0015285605812504466	0.0
29	0.0	0.0	0.0	0.002560338973594498	0.0
30	0.0	0.0	0.0	0.003744973424063594	0.0
31	0.0	0.0	0.0	0.00783387297890854	0.0
32	0.0	0.0	0.0	0.011540632388440872	0.0
33	0.0	0.0	0.0	0.015629531943285816	0.0
34	0.0	0.0	0.0	0.02032985573063094	0.0
35	0.0	0.0	0.0	0.027666946520633083	0.0
36	0.0	0.0	0.0	0.03530974942688532	0.0
37	0.0	0.0	0.0	0.04558931933579457	0.0
38	0.0	0.0	0.0	0.05709173770970418	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2795	0.0	30.856035	1
GTATCAA	6965	0.0	25.964895	1
ACGCGTA	60	0.0074122343	18.33424	6
GTCGCGC	60	0.007413869	18.33354	30
TATCAAC	9955	0.0	18.121752	2
ATCAACG	10225	0.0	17.729635	3
GTGGTAT	810	0.0	17.654858	1
CGCGATC	75	0.0012908217	17.601208	23
TCAACGC	10380	0.0	17.401632	4
CAACGCA	10515	0.0	17.179531	5
AACGCAG	10755	0.0	16.713053	6
CGAATCG	135	1.0884542E-6	16.293676	37
CGGTTAC	165	3.1124728E-8	16.001099	28
GTACCGA	195	8.931238E-10	15.795652	6
CCGTAAC	85	0.0029798176	15.5304785	24
GTACATG	9730	0.0	15.036428	1
TACATGG	9965	0.0	14.482852	2
TAGACAG	630	0.0	14.319264	5
GTATGCG	170	7.595845E-7	14.235999	11
ACGCAGA	12465	0.0	14.208486	7
>>END_MODULE