FastQCFastQC Report
Mon 6 Feb 2017
SRR2077473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592864
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.025.616631
GTATCAA34700.025.5506231
CAACGCA45900.018.7394335
TATCAAC46550.018.7147772
TCAACGC46200.018.6184674
ATCAACG46800.018.4734273
AACGCAG47950.018.0296766
AGCGTAC3150.017.46213323
GTACCGA2950.017.1510146
TACCGAT3100.015.6118147
ACGCAGA54950.015.4129377
CGCAGAG56150.015.0051888
GTACATG64750.014.9499191
CGTACAA3550.014.87478525
TGCGATA2407.2759576E-1114.66734213
TACATGG64600.014.4732332
CGACTCA3050.014.42717321
GCGTACA3550.014.25500324
AGAGTAC56300.014.06814311
CAGCGTA3650.013.8639222
Produced by FastQC (version 0.11.5)