FastQCFastQC Report
Mon 6 Feb 2017
SRR2077473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592864
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.12009885593690993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.027.4698831
GTATCAA39100.025.9995351
CGTGCCG2400.022.00181410
TCGATCG550.004494858419.9923941
TACCGCG1001.122733E-619.80316217
CGAATCG2350.017.78389537
GTGCCGA2900.017.4500511
TATCAAC58450.017.1651332
CGGCACG3000.016.86838322
TCAACGC59350.016.8310184
ATCAACG59650.016.782933
CAACGCA59850.016.5443135
AACGCAG62100.015.942426
CGACTCA2700.015.48305721
CGCACGA2152.1645974E-1015.34654935
TACCGAT2900.015.1727877
AACGACT2800.014.93095519
GCGCTAT1057.161403E-414.66702656
TGCGATA2753.6379788E-1214.40285413
GTACATG65700.014.3343751
Produced by FastQC (version 0.11.5)