FastQCFastQC Report
Mon 6 Feb 2017
SRR2077472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444922
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.13863884291139247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.026.581441
GTATCAA52150.025.1871811
TATCAAC68650.018.6834032
TCAACGC69500.018.2971574
ATCAACG69600.018.2698083
CAACGCA70350.018.1375775
AACGCAG72900.017.6217986
CGTGCCG2450.017.06341610
ACGCAGA83600.015.0784747
GTATAAA11100.014.8661271
CGCAGAG85450.014.6490438
GCGATAC2652.3646862E-1114.11420114
TTATACT9300.013.9575664
AGAGTAC84550.013.94836611
GTACATG82900.013.6682271
CAGAGTA90500.013.37188310
TACATGG82650.013.1762042
TGCGATA2704.6929927E-1013.03852113
CGCACGA3550.013.01443335
GCAGAGT94000.012.9908429
Produced by FastQC (version 0.11.5)