FastQCFastQC Report
Mon 6 Feb 2017
SRR2077472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444922
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.14435740489915302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.030.3682331
GTATCAA62900.026.478721
CGCACGA3350.019.6985235
TATCAAC91550.017.8311122
ATCAACG93250.017.4357683
TCAACGC94100.017.208634
CAACGCA95400.016.974875
ATCCGCG800.001988466116.50110426
AACGCAG98900.016.3951996
CGTGCCG3300.016.00107210
GCGATAC2001.2769306E-915.40237314
ACGCAGA115050.013.9792077
CGCAGAG117150.013.6722778
CGGCACG3250.013.53936922
GCACCGT5200.013.5391716
GTACATG83350.013.5150031
AGCGTAC2805.820766E-1113.35803823
GAGTACT75600.013.21310112
AGAGTAC116500.013.20126811
TACCCCG1701.1853725E-512.9427955
Produced by FastQC (version 0.11.5)