##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077472_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3444922 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.494082014048505 31.0 31.0 34.0 27.0 34.0 2 30.454938312101117 31.0 31.0 34.0 26.0 34.0 3 30.61983261159469 31.0 31.0 34.0 27.0 34.0 4 34.06934264404245 37.0 35.0 37.0 30.0 37.0 5 33.70315351116803 37.0 35.0 37.0 28.0 37.0 6 33.64411530943226 37.0 35.0 37.0 28.0 37.0 7 33.45879877686635 37.0 35.0 37.0 27.0 37.0 8 33.42999899562312 37.0 35.0 37.0 27.0 37.0 9 34.877835550413046 39.0 35.0 39.0 27.0 39.0 10 34.737388829122985 39.0 35.0 39.0 27.0 39.0 11 34.677506776641096 39.0 35.0 39.0 27.0 39.0 12 34.60662186255596 39.0 35.0 39.0 27.0 39.0 13 34.57186316555208 39.0 35.0 39.0 27.0 39.0 14 35.71770681600338 40.0 36.0 41.0 27.0 41.0 15 35.72323204995643 40.0 36.0 41.0 27.0 41.0 16 35.670032877377196 40.0 36.0 41.0 27.0 41.0 17 35.64300381837383 40.0 36.0 41.0 27.0 41.0 18 35.640997386878425 40.0 36.0 41.0 27.0 41.0 19 35.68412492358318 40.0 36.0 41.0 26.0 41.0 20 35.683663374671475 40.0 36.0 41.0 27.0 41.0 21 35.62831814479399 40.0 36.0 41.0 26.0 41.0 22 35.6303895995323 40.0 36.0 41.0 26.0 41.0 23 35.56647523514321 40.0 36.0 41.0 26.0 41.0 24 35.54894972948589 40.0 35.0 41.0 26.0 41.0 25 35.52847989011072 40.0 35.0 41.0 26.0 41.0 26 35.44863105753918 40.0 35.0 41.0 25.0 41.0 27 35.38920707058099 40.0 35.0 41.0 25.0 41.0 28 35.327477080758285 40.0 35.0 41.0 25.0 41.0 29 35.25065589293459 40.0 35.0 41.0 25.0 41.0 30 35.119290073911685 39.0 35.0 41.0 24.0 41.0 31 35.03549833639194 39.0 35.0 41.0 24.0 41.0 32 34.950332982865795 39.0 35.0 41.0 24.0 41.0 33 34.85412499905658 39.0 34.0 41.0 24.0 41.0 34 34.82504683705466 39.0 34.0 41.0 24.0 41.0 35 34.755404041078435 39.0 34.0 41.0 23.0 41.0 36 34.64948640346574 39.0 34.0 41.0 22.0 41.0 37 34.56265453905778 39.0 34.0 41.0 22.0 41.0 38 34.47907064368947 39.0 34.0 41.0 21.0 41.0 39 34.3905115993918 39.0 34.0 41.0 19.0 41.0 40 34.295302767377606 39.0 34.0 41.0 19.0 41.0 41 34.228420556401566 39.0 34.0 41.0 18.0 41.0 42 34.11209397484181 39.0 33.0 41.0 16.0 41.0 43 34.20488620642209 39.0 34.0 41.0 16.0 41.0 44 34.13489216882124 39.0 34.0 41.0 15.0 41.0 45 34.06081095595198 39.0 34.0 41.0 15.0 41.0 46 33.8989245620075 39.0 33.0 41.0 9.0 41.0 47 33.74632429994061 39.0 33.0 41.0 2.0 41.0 48 33.58310173641087 39.0 33.0 41.0 2.0 41.0 49 33.425907466119696 39.0 33.0 41.0 2.0 41.0 50 33.279505602739334 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48816588847912357 1101 2 0.6698022470576248 1101 3 0.4776539372880819 1101 4 0.4902756794081 1101 5 0.5290254107329062 1101 6 0.5399850623232894 1101 7 0.6304592066916825 1101 8 0.56183529887128 1101 9 0.6085360751844959 1101 10 0.6938738018575492 1101 11 0.7459537110856687 1101 12 0.6471954844728671 1101 13 0.6949603664985702 1101 14 0.7589496010862433 1101 15 0.7140635004062332 1101 16 0.7838358109859342 1101 17 0.7854137209356367 1101 18 0.7687207693920612 1101 19 0.8452311863686788 1101 20 0.8774317505480411 1101 21 0.9184248763997473 1101 22 0.8795469781507279 1101 23 0.9348841337923588 1101 24 0.846380582471518 1101 25 0.8266037293428852 1101 26 0.9407449972522386 1101 27 0.8383675199796627 1101 28 0.9158783039601772 1101 29 1.0022828579348158 1101 30 1.059666669516794 1101 31 1.015944122818894 1101 32 1.0209441471799678 1101 33 1.026892918268416 1101 34 0.8369455151588241 1101 35 0.8818942016382749 1101 36 0.8547575632645774 1101 37 0.9174885014387755 1101 38 1.0206522973671497 1101 39 0.8695352868582802 1101 40 1.0468681034743739 1101 41 1.066774221297706 1101 42 1.0135747763659992 1101 43 0.9988126697087694 1101 44 1.0445713767699374 1101 45 1.0892744362624711 1101 46 1.061670817365055 1101 47 1.035285388424903 1101 48 1.1092272183285772 1101 49 1.1013960308833504 1101 50 1.1831211450521977 1102 1 0.5981828553824187 1102 2 0.7168720945253035 1102 3 0.6303679993033313 1102 4 0.5979364634194653 1102 5 0.6391104150560949 1102 6 0.6379853358497769 1102 7 0.5948707025144344 1102 8 0.6875920860915343 1102 9 0.799256092367095 1102 10 0.8336031574915523 1102 11 0.8897185003325916 1102 12 0.8521759383432652 1102 13 0.7961868528580425 1102 14 0.8640835923517542 1102 15 0.89799021641781 1102 16 0.976089595839909 1102 17 0.906555207561297 1102 18 0.8975348806053631 1102 19 0.91743931157405 1102 20 0.9517105997908857 1102 21 0.9495042091786843 1102 22 0.9023198942551929 1102 23 0.9008144818318655 1102 24 0.9980026037404954 1102 25 0.9475701314782299 1102 26 0.8939467257928015 1102 27 0.8569548153166764 1102 28 0.9217063457491506 1102 29 0.9832044999382035 1102 30 0.9334461263923117 1102 31 0.9678055256609994 1102 32 1.109104580563418 1102 33 1.1485259264907342 1102 34 1.1118870236298903 1102 35 1.0450890094078957 1102 36 0.9719506995854061 1102 37 1.0079267542102954 1102 38 0.9561298764557762 1102 39 0.9203203446017412 1102 40 0.8983375970097853 1102 41 0.9401577302203634 1102 42 0.9304561108448368 1102 43 0.8626757586856542 1102 44 0.9076025275821635 1102 45 0.802745455304688 1102 46 0.8715040488143657 1102 47 0.9240730052014428 1102 48 0.8736752455417118 1102 49 0.9908944264393753 1102 50 0.8871207356803339 1103 1 0.42053638644929947 1103 2 0.6048053738245684 1103 3 0.5162829786267871 1103 4 0.4563075034180386 1103 5 0.4412790378430955 1103 6 0.5664168370556197 1103 7 0.5247322591063437 1103 8 0.46854913038061596 1103 9 0.5818937809548146 1103 10 0.6115720291955995 1103 11 0.7062289392216101 1103 12 0.7053396529465772 1103 13 0.6601828028220922 1103 14 0.7265085616798999 1103 15 0.8124842409505604 1103 16 0.8707311765906525 1103 17 0.9167031233447958 1103 18 0.8560899173738008 1103 19 0.8194890105505408 1103 20 0.7770019184672279 1103 21 0.8648676417330137 1103 22 0.8334297386167719 1103 23 0.8767353493267862 1103 24 0.85333710921158 1103 25 0.8214070305791878 1103 26 0.7973002505233922 1103 27 0.8748304503868525 1103 28 0.915180002766256 1103 29 0.9121860371273129 1103 30 0.9042315434230304 1103 31 0.8401473269528879 1103 32 0.9193193785849374 1103 33 0.92352315340856 1103 34 0.8538852215272641 1103 35 0.9091721406478541 1103 36 0.9163099985105347 1103 37 0.9629641181050204 1103 38 0.8919648009237306 1103 39 0.9855888892691311 1103 40 0.8739687127247606 1103 41 0.9241004965524766 1103 42 0.8706442208608891 1103 43 0.9404749322651753 1103 44 0.8720234824492081 1103 45 0.8320614571225207 1103 46 0.9218055074670417 1103 47 0.8502952015695584 1103 48 0.9871775181713005 1103 49 0.9998744032210567 1103 50 1.0881148007119137 1104 1 0.4917668698113893 1104 2 0.6782305768844985 1104 3 0.5963959761883402 1104 4 0.5873196962391134 1104 5 0.5703008802887055 1104 6 0.5287977790918106 1104 7 0.45809632843508297 1104 8 0.5125540042950831 1104 9 0.5459612348835563 1104 10 0.5763739330517055 1104 11 0.5304237821213391 1104 12 0.5481233253033437 1104 13 0.6066956238537884 1104 14 0.7031521314636535 1104 15 0.6742785753300211 1104 16 0.7314696648022547 1104 17 0.7929585774092374 1104 18 0.7504004004264004 1104 19 0.666033390907522 1104 20 0.7631237758518168 1104 21 0.6624278127634255 1104 22 0.6808850392442665 1104 23 0.7792455283623525 1104 24 0.7437819841021422 1104 25 0.7249706006724352 1104 26 0.7338792494217117 1104 27 0.7470996239045178 1104 28 0.7463104575002717 1104 29 0.8004803099058293 1104 30 0.7829728337337087 1104 31 0.8002621279044533 1104 32 0.7955120413468393 1104 33 0.9034857345536693 1104 34 0.7988351045449846 1104 35 0.8804922132119799 1104 36 0.8522287959648409 1104 37 0.9004812666775734 1104 38 0.9481049124244194 1104 39 0.8880008638978723 1104 40 0.8826425425597293 1104 41 0.8680469834832607 1104 42 0.880270484647447 1104 43 0.878327835752863 1104 44 0.8606133207333357 1104 45 0.8844049267377798 1104 46 0.8429164939213791 1104 47 0.8381101178071475 1104 48 0.9637072601198824 1104 49 0.9054185032490949 1104 50 1.0427797778660022 1105 1 0.45757817218589736 1105 2 0.6190130377211105 1105 3 0.44321473847852033 1105 4 0.4161706043455595 1105 5 0.5592016390857424 1105 6 0.5401344915482582 1105 7 0.5646090926794116 1105 8 0.580827976393202 1105 9 0.6820311667690007 1105 10 0.6942261871940403 1105 11 0.6208827879609871 1105 12 0.6020524695531364 1105 13 0.6760129638015258 1105 14 0.7426448653841717 1105 15 0.7077301545832881 1105 16 0.7534173734714358 1105 17 0.7614113728895475 1105 18 0.7128094830696128 1105 19 0.7032330544570158 1105 20 0.7212115248786901 1105 21 0.7185057466041869 1105 22 0.7563593093180074 1105 23 0.8034631376926811 1105 24 0.7702336797155951 1105 25 0.7439060277873963 1105 26 0.7711191858738502 1105 27 0.746139579301726 1105 28 0.8860094091476469 1105 29 0.7712958310678886 1105 30 0.9210516598641121 1105 31 0.9024211794110855 1105 32 0.917163543182582 1105 33 0.9025713715555028 1105 34 0.7829843917365338 1105 35 0.8897974464381377 1105 36 0.9810344025376097 1105 37 0.957041525172599 1105 38 0.9207470399563533 1105 39 0.9740715120708714 1105 40 1.033284540867541 1105 41 1.0611175445323013 1105 42 1.060059977966091 1105 43 0.9870713241142113 1105 44 1.083192809649006 1105 45 1.0135769363249167 1105 46 1.0453634053186533 1105 47 1.0892779588930495 1105 48 1.047926889673498 1105 49 1.1131583411751436 1105 50 1.1961018610992014 1106 1 0.4291887964988135 1106 2 0.5900094686367154 1106 3 0.4596329304269702 1106 4 0.4529260309342007 1106 5 0.4327857282828589 1106 6 0.47980504048820194 1106 7 0.38666503063686974 1106 8 0.4168560751463275 1106 9 0.4915383528101174 1106 10 0.5544480552763389 1106 11 0.5350815781175129 1106 12 0.5860848831608791 1106 13 0.5851708115767309 1106 14 0.5976834718262864 1106 15 0.5969128382948128 1106 16 0.6466788665807073 1106 17 0.7378012278767514 1106 18 0.657245347984265 1106 19 0.683692706730838 1106 20 0.6852129714306656 1106 21 0.66225258155076 1106 22 0.6710671756076891 1106 23 0.7546087716781145 1106 24 0.6333467051433033 1106 25 0.7257964428232313 1106 26 0.7327263668393513 1106 27 0.6529845588781242 1106 28 0.6942968600208275 1106 29 0.6733153658641555 1106 30 0.7088535006995613 1106 31 0.7634129701709185 1106 32 0.6845814305937736 1106 33 0.7946321630755264 1106 34 0.6958480196058474 1106 35 0.6564327942162222 1106 36 0.7762041849434311 1106 37 0.7617634964976432 1106 38 0.7255968390655809 1106 39 0.7051450693544439 1106 40 0.7461044636027481 1106 41 0.6649268269202437 1106 42 0.6326589496914252 1106 43 0.6167703830479354 1106 44 0.6620367418017992 1106 45 0.647075068727105 1106 46 0.5981228272284937 1106 47 0.5678850816042669 1106 48 0.6870058980586506 1106 49 0.7298165869251747 1106 50 0.7417611813812641 1107 1 0.3251521273925917 1107 2 0.534814964843676 1107 3 0.3890471649682148 1107 4 0.44417450999823416 1107 5 0.4876718220309968 1107 6 0.4261589744249292 1107 7 0.4446273823970657 1107 8 0.3746643673772496 1107 9 0.36321131424491426 1107 10 0.45600290180303915 1107 11 0.45299411585347116 1107 12 0.508007922523241 1107 13 0.5350545518840448 1107 14 0.5337251334066053 1107 15 0.4632183435862274 1107 16 0.5198864971228616 1107 17 0.5647564006488324 1107 18 0.5803408139343205 1107 19 0.5279635900404855 1107 20 0.4770332457697606 1107 21 0.5724932286816866 1107 22 0.4742834335402648 1107 23 0.4291855000944551 1107 24 0.4532718251290433 1107 25 0.4399788620241267 1107 26 0.49626400996914555 1107 27 0.5397972207304065 1107 28 0.5599341258052064 1107 29 0.5181995206495884 1107 30 0.6201666809333801 1107 31 0.5718488844727432 1107 32 0.528315018253906 1107 33 0.5049224269277559 1107 34 0.4589483315432119 1107 35 0.48354559642329065 1107 36 0.5087424134526017 1107 37 0.513396071865948 1107 38 0.5018253195604103 1107 39 0.4894223387498897 1107 40 0.45446161996490275 1107 41 0.44711978238201056 1107 42 0.534128842398367 1107 43 0.5297634416750157 1107 44 0.4569502365279874 1107 45 0.49823274727491196 1107 46 0.6022990136437159 1107 47 0.6606846030807603 1107 48 0.5930172005362664 1107 49 0.6521458006488317 1107 50 0.7342019592715232 1108 1 0.299325277127938 1108 2 0.49603193895139697 1108 3 0.33906776864710153 1108 4 0.3079859207181457 1108 5 0.2637814906498974 1108 6 0.2810295441146451 1108 7 0.2900448382779075 1108 8 0.33960784403046773 1108 9 0.29086920191379306 1108 10 0.23915234981890876 1108 11 0.315103850876163 1108 12 0.36799881102935217 1108 13 0.375947926562759 1108 14 0.36083382122545515 1108 15 0.4161230084455596 1108 16 0.3964198198334472 1108 17 0.40174235561861593 1108 18 0.4054215676822821 1108 19 0.40493949995309464 1108 20 0.30032710213082225 1108 21 0.3848085693224874 1108 22 0.3316392925384122 1108 23 0.4126342679113648 1108 24 0.3938852599984912 1108 25 0.3524382765472822 1108 26 0.29446658992051766 1108 27 0.39338600872611096 1108 28 0.3988694963779835 1108 29 0.41837713158852097 1108 30 0.5398509117053365 1108 31 0.44376082452722443 1108 32 0.4316745647921252 1108 33 0.4358028186528671 1108 34 0.3592085108131968 1108 35 0.35677719835749144 1108 36 0.4254980694618311 1108 37 0.37391295380786005 1108 38 0.40369545909181426 1108 39 0.4521346550087344 1108 40 0.5101070890774864 1108 41 0.4528015309369735 1108 42 0.3344813760049874 1108 43 0.37137664461997844 1108 44 0.4042765290810948 1108 45 0.43611367661935674 1108 46 0.47208358850392074 1108 47 0.4986701760018093 1108 48 0.4702575734965464 1108 49 0.5109950816043565 1108 50 0.49816937002258044 1109 1 0.1810872471231164 1109 2 0.3338440949855226 1109 3 0.25259513579775117 1109 4 0.23282098604313006 1109 5 0.2543261829577901 1109 6 0.2886954190176354 1109 7 0.2631641099380744 1109 8 0.21620135289232678 1109 9 0.08003729978190677 1109 10 0.2599413524545824 1109 11 0.10165589454631885 1109 12 0.2234184340527534 1109 13 0.13784703399782217 1109 14 0.2247016357218854 1109 15 0.2443016030781422 1109 16 0.27662005288034663 1109 17 0.29478726824395096 1109 18 0.2522325019372147 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-1.6760277292052344 2216 49 -1.6071375752937165 2216 50 -1.645825201112725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87115.0 3 13611.0 4 23468.0 5 13954.0 6 10555.0 7 8194.0 8 6965.0 9 6175.0 10 5736.0 11 5758.0 12 5884.0 13 6419.0 14 6970.0 15 7339.0 16 8123.0 17 8641.0 18 9562.0 19 10668.0 20 12496.0 21 15637.0 22 19692.0 23 24201.0 24 28220.0 25 31903.0 26 34302.0 27 39412.0 28 46382.0 29 53062.0 30 60392.0 31 71954.0 32 88007.0 33 109076.0 34 138091.0 35 174515.0 36 226524.0 37 304263.0 38 481191.0 39 1240465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.07490625395568 18.39010548099356 14.349764197778894 26.18522406727186 2 14.544025819044332 20.211274699200178 38.6200292084035 26.62467027335199 3 17.378751478994502 25.014107778785384 29.694116649849573 27.913024092370538 4 12.067892454265195 17.00913825235278 37.04141822889754 33.881551064484476 5 13.656098550440134 37.69175080591602 34.43300711436327 14.219143529280576 6 31.958443196497623 37.35234347166728 17.33809864354742 13.35111468828768 7 27.871909879413543 31.982111077525342 21.9722357000847 18.17374334297642 8 26.440267444642394 34.60337987405899 19.842678255779653 19.113674425518965 9 26.40956198065617 15.305226155662973 19.0660906899148 39.219121173766055 10 14.192621070542938 28.984134082105545 33.984192156475586 22.839052690875935 11 34.67300654169865 22.98306336883977 23.74296557586085 18.600964513600733 12 23.400457951646462 24.72355198564291 30.55800696655675 21.317983096153874 13 28.084365096949533 21.76557823433465 26.12870637067874 24.02135029803708 14 22.186780256488962 20.83617806412718 27.486328594067494 29.490713085316365 15 23.46157509527676 28.768644450716856 24.123953291447567 23.64582716255882 16 23.369733620524176 27.96306401302639 25.452167304443684 23.215035062005747 17 22.185452965618826 27.648635242649988 26.920110263240126 23.245801528491057 18 22.189304776182343 27.130822985199636 28.048665028313003 22.631207210305014 19 23.188967287839997 26.77898417612609 28.01155315598939 22.020495380044522 20 23.336657598026118 26.140307977538267 28.091072461613166 22.43196196282245 21 23.494570381746076 26.112758092733323 27.871140080433626 22.52153144508697 22 23.152380609420213 25.85822052829119 28.017101681940314 22.972297180348285 23 23.0706815618317 25.87235337644521 27.859027183250255 23.197937878472832 24 22.7047560746122 26.101607893336805 27.94161890535012 23.25201712670088 25 22.976613521254535 25.92279932662925 27.986180624706154 23.114406527410065 26 22.782296472862274 26.27054902726086 28.067514415682776 22.87964008419409 27 22.939439691034654 26.37115858809645 27.676112598457436 23.01328912241146 28 22.936858560259363 26.128560779178766 27.91928498807762 23.015295672484253 29 23.096006609429473 26.197356518607894 27.753563530817626 22.953073341145007 30 23.073907509693875 26.31001992351762 27.819717864870285 22.796354701918215 31 23.124749181555096 25.902641499352736 27.829275616004296 23.143333703087876 32 22.877401703961574 26.26427287776467 27.703386058131912 23.154939360141842 33 23.113004304107367 26.104103419361284 27.59015862249982 23.19273365403153 34 23.224394659871983 26.134335889662285 27.68259209084559 22.958677359620143 35 23.18637467721415 26.12943835987915 27.707728336692572 22.97645862621413 36 23.109265290372708 26.224581373140808 27.522017596656617 23.144135739829863 37 23.14206461974942 25.919989176427137 27.69202986702219 23.24591633680125 38 23.143114876943173 26.092187592090475 27.731967832098398 23.032729698867957 39 23.374868629625546 26.258862056752 27.36086353158405 23.005405782038405 40 23.24433211638147 26.047472734704495 27.799163461717107 22.909031687196922 41 23.05573554241071 26.151741692155127 27.52623163263173 23.26629113280243 42 23.281063936148037 26.172795567010134 27.468721677652567 23.077418819189262 43 23.174435360358007 26.013134961966433 27.505361623663365 23.307068054012188 44 23.202706684413197 26.24723315252968 27.242645084409713 23.307415078647413 45 23.29192456431964 26.303354406384965 27.09752725959565 23.30719376969974 46 23.36649356002659 26.15877430048565 27.00237162870222 23.47236051078554 47 23.304734684541113 26.32506782454619 26.870421879417737 23.49977561149496 48 23.134341139345043 26.53149909039687 26.941524435043128 23.39263533521495 49 23.33888063035408 26.27296823352408 26.91992066000933 23.468230476112513 50 23.4372128923786 26.38802247600016 26.831109817095843 23.34365481452539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 895.0 1 652.5 2 410.0 3 591.0 4 772.0 5 917.0 6 1062.0 7 1050.5 8 1039.0 9 1365.5 10 1692.0 11 2564.0 12 3436.0 13 6267.5 14 9099.0 15 12674.0 16 16249.0 17 18485.0 18 20721.0 19 22158.5 20 23596.0 21 25258.5 22 26921.0 23 30995.0 24 35069.0 25 41515.0 26 47961.0 27 55447.5 28 62934.0 29 70275.0 30 77616.0 31 85798.0 32 93980.0 33 104996.0 34 116012.0 35 127938.5 36 139865.0 37 149902.0 38 159939.0 39 169617.0 40 179295.0 41 188926.5 42 198558.0 43 204829.0 44 211100.0 45 217924.0 46 224748.0 47 231601.5 48 238455.0 49 239666.0 50 240877.0 51 241038.0 52 241199.0 53 233457.0 54 225715.0 55 217492.5 56 209270.0 57 195501.0 58 181732.0 59 163784.0 60 145836.0 61 127303.0 62 108770.0 63 92626.5 64 76483.0 65 63387.0 66 50291.0 67 41171.0 68 32051.0 69 25248.5 70 18446.0 71 14341.0 72 10236.0 73 8203.0 74 6170.0 75 4663.5 76 3157.0 77 2376.0 78 1595.0 79 1189.0 80 783.0 81 606.0 82 429.0 83 329.0 84 229.0 85 175.0 86 121.0 87 90.5 88 60.0 89 41.5 90 23.0 91 18.0 92 13.0 93 9.0 94 5.0 95 4.0 96 3.0 97 3.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014804399054608492 2 6.676493691294027E-4 3 4.0639526816572334E-4 4 0.0017997504733053462 5 0.038462409308541674 6 0.02510942192595362 7 0.029115318140730036 8 0.022351739749114783 9 0.029986165143942305 10 0.030827983913714156 11 0.038346296374780035 12 0.038520465775422494 13 0.03550152949761998 14 0.04124911971882092 15 0.03686585646931919 16 0.042119966722033185 17 0.04429708423006384 18 0.04725796404098555 19 0.049551194482777845 20 0.04510987476639529 21 0.04603877823648837 22 0.04406485836254057 23 0.042903729024924224 24 0.04092980915097642 25 0.0392171433779923 26 0.04209093848859277 27 0.04148134558634419 28 0.040610498583131927 29 0.03939131277863475 30 0.03637237650083224 31 0.03471776719472894 32 0.030915068614035385 33 0.022351739749114783 34 0.019158634070669815 35 0.019971424607001263 36 0.021626033913104564 37 0.01706860126296038 38 0.017968476499613053 39 0.01341104384946887 40 0.0072570583601022035 41 0.006560380757532392 42 0.005921759621843397 43 0.0020319763408286166 44 0.003396303312527831 45 0.002322258675232705 46 9.289034700930819E-4 47 1.1611293376163524E-4 48 3.773670347253145E-4 49 2.902823344040881E-5 50 8.708470032122642E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3444922.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.99630114645902 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96746677065566 44.05832080164564 2 13.162002686440832 15.266949429866502 3 4.668540347237886 8.122742156784083 4 2.206401731664488 5.118525571187293 5 1.116193172608541 3.2367537688113233 6 0.6877742420315562 2.393301723698384 7 0.44371937175121373 1.8013857616020694 8 0.31391838757795126 1.456488427310536 9 0.23076498927654776 1.2045164230927845 >10 1.1078131846907904 11.31146949874432 >50 0.06616891524852271 2.642678291659689 >100 0.02833064081274205 2.762736634041406 >500 7.54633336791421E-4 0.30353139775479127 >1k 1.5092666647824096E-4 0.3206001138011149 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4973 0.14435740489915302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.902823344040881E-5 7 0.0 0.0 0.0 0.0 2.902823344040881E-5 8 0.0 0.0 0.0 0.0 2.902823344040881E-5 9 0.0 0.0 0.0 0.0 5.805646688081762E-5 10 0.0 0.0 0.0 0.0 5.805646688081762E-5 11 0.0 0.0 0.0 0.0 5.805646688081762E-5 12 0.0 0.0 0.0 0.0 1.4514116720204407E-4 13 0.0 0.0 0.0 2.902823344040881E-5 1.4514116720204407E-4 14 0.0 0.0 0.0 2.902823344040881E-5 1.4514116720204407E-4 15 0.0 0.0 0.0 2.902823344040881E-5 1.4514116720204407E-4 16 0.0 0.0 0.0 2.902823344040881E-5 1.7416940064245287E-4 17 0.0 0.0 0.0 2.902823344040881E-5 2.0319763408286167E-4 18 0.0 0.0 0.0 2.902823344040881E-5 2.0319763408286167E-4 19 0.0 0.0 0.0 2.902823344040881E-5 2.0319763408286167E-4 20 0.0 2.902823344040881E-5 0.0 5.805646688081762E-5 2.0319763408286167E-4 21 0.0 2.902823344040881E-5 0.0 8.708470032122643E-5 2.0319763408286167E-4 22 0.0 2.902823344040881E-5 0.0 1.1611293376163524E-4 2.0319763408286167E-4 23 0.0 5.805646688081762E-5 0.0 1.7416940064245287E-4 2.0319763408286167E-4 24 0.0 5.805646688081762E-5 0.0 3.193105678444969E-4 2.0319763408286167E-4 25 0.0 5.805646688081762E-5 0.0 3.193105678444969E-4 2.0319763408286167E-4 26 0.0 5.805646688081762E-5 0.0 5.225082019273586E-4 2.0319763408286167E-4 27 0.0 5.805646688081762E-5 0.0 6.966776025698115E-4 2.0319763408286167E-4 28 0.0 8.708470032122643E-5 0.0 0.0012772422713779876 2.0319763408286167E-4 29 0.0 8.708470032122643E-5 0.0 0.0019158634070669815 2.0319763408286167E-4 30 0.0 8.708470032122643E-5 0.0 0.0026996257099580192 2.0319763408286167E-4 31 0.0 8.708470032122643E-5 0.0 0.004296178549180504 2.612541009636793E-4 32 0.0 8.708470032122643E-5 0.0 0.006502324290651573 2.612541009636793E-4 33 0.0 8.708470032122643E-5 0.0 0.009318062934371228 2.612541009636793E-4 34 0.0 8.708470032122643E-5 0.0 0.0121918580449717 2.612541009636793E-4 35 0.0 8.708470032122643E-5 0.0 0.015326907256535852 2.612541009636793E-4 36 0.0 8.708470032122643E-5 0.0 0.0197682269729184 2.612541009636793E-4 37 0.0 8.708470032122643E-5 0.0 0.02560290189444057 2.612541009636793E-4 38 0.0 8.708470032122643E-5 0.0 0.032511621453257866 2.612541009636793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2485 0.0 30.368233 1 GTATCAA 6290 0.0 26.47872 1 CGCACGA 335 0.0 19.69852 35 TATCAAC 9155 0.0 17.831112 2 ATCAACG 9325 0.0 17.435768 3 TCAACGC 9410 0.0 17.20863 4 CAACGCA 9540 0.0 16.97487 5 ATCCGCG 80 0.0019884661 16.501104 26 AACGCAG 9890 0.0 16.395199 6 CGTGCCG 330 0.0 16.001072 10 GCGATAC 200 1.2769306E-9 15.402373 14 ACGCAGA 11505 0.0 13.979207 7 CGCAGAG 11715 0.0 13.672277 8 CGGCACG 325 0.0 13.539369 22 GCACCGT 520 0.0 13.539171 6 GTACATG 8335 0.0 13.515003 1 AGCGTAC 280 5.820766E-11 13.358038 23 GAGTACT 7560 0.0 13.213101 12 AGAGTAC 11650 0.0 13.201268 11 TACCCCG 170 1.1853725E-5 12.942795 5 >>END_MODULE