FastQCFastQC Report
Mon 6 Feb 2017
SRR2077471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3718308
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59750.023.8215371
GGTATCA26900.022.3261471
TATCAAC78650.017.6211222
ATCAACG80850.017.3581393
TCAACGC80600.017.3315054
CAACGCA80250.017.2421535
CGCACGA2700.017.11196535
AACGCAG82650.016.7935756
TATACCG1857.590643E-915.458155
GTACATG101150.015.3329841
ACGCAGA93600.014.7126277
TACATGG103450.014.5876512
CGCAGAG96300.014.18590458
AGAGTAC89450.013.97088911
ACATGGG106650.013.9221223
GTATAGT5550.013.8732581
GAGTACT45550.013.52463912
CGACAGG4400.013.50121934
ACCGTGC6600.013.3323838
TTATACT8300.013.2522464
Produced by FastQC (version 0.11.5)