FastQCFastQC Report
Mon 6 Feb 2017
SRR2077471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3718308
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.12043112082162101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.024.905091
GTATCAA68900.023.120581
TATCAAC96950.016.316662
TCAACGC99400.015.9589714
ATCAACG99850.015.9088693
AATAGCG1401.5861315E-615.7163365
CAACGCA101000.015.7070015
AACGCAG104250.015.2785966
GCGATAC2750.015.20239414
TATACCG1503.2502994E-614.6685815
CGAATCG3400.014.2329437
CGTACAC2855.456968E-1213.89561753
GTACATG98500.013.6484811
CGTACTA2602.582965E-1013.53913729
CGTGCCG3750.013.49418710
TACATGG100900.013.1703072
GACCGTA2857.8216544E-1113.12381526
ACGCAGA121750.013.0828467
TGCGATA3051.8189894E-1112.98565113
GCGCGTT1701.18881835E-512.9392136
Produced by FastQC (version 0.11.5)