FastQCFastQC Report
Mon 6 Feb 2017
SRR2077470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174362
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113880.969718025617314No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA83380.7100025375480473No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT66550.5666906797052357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.5409745887554264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.2907961940185394No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT33230.28296215306694184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.24115221711874193No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC26850.2286347821199937No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT26180.2229295566443737No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT23360.19891651807534644No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT22710.1933815978378047No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA21420.1823969099817603No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG19270.164089096888353No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18530.15778780307945933No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA18240.1553183771273253No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC17040.14510006284263285No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA16310.13888392165277827No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC16120.13726602189103532No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA15900.13539266427217503No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT15840.13488174855794038No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG15780.13437083284370577No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT15510.13207171212964997No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT14920.12704770760634285No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14850.12645163927306913No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG14420.12279007665438767No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA14400.12261977141630946No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA14170.12066126117841007No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC14030.1194691245118626No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT13820.11768091951204145No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG13810.11759576689300232No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA13680.11648878284549397No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC13530.11521149355990742No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA12740.10848443665581822No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGT12620.10746260522734898No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.10695168951311436No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG11920.10150192189461171No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC11890.1012464640374944No Hit
ATATTATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA11860.10099100618037708No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC11850.10090585356133798No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11820.10065039570422067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATGC207.8644615E-443.9910138
CGCTTCA250.00235463235.194318
GATTGCC502.3612138E-630.800279
AGACGCT300.005747093429.3310895
CTAGGTC300.005748290629.3298418
GAACTCG407.0193975E-427.50844431
TGGCATG407.0228963E-427.506137
TACGCCG407.035156E-427.4978945
CGCAGAA959.622454E-1025.4771652
AATGCCG701.131295E-625.1452229
GACATAT1950.024.826021
CGTTGTT450.001398118124.44465824
GTCGTTG450.001398465224.44361722
AGTTTAC1001.7444108E-924.20124214
GGTATCA15850.024.1567781
TCGGCAA551.5902628E-424.00737235
GGTCATT551.5934849E-424.0002117
CGTTAAT551.5934849E-424.0002126
GTATCAA37650.023.9628181
GTATGGT1202.3646862E-1123.8386211
Produced by FastQC (version 0.11.5)