##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077470_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174362 Sequences flagged as poor quality 0 Sequence length 50 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.16693915504759 31.0 28.0 34.0 15.0 34.0 2 28.258013287214673 31.0 28.0 34.0 15.0 34.0 3 28.36533879672537 31.0 28.0 34.0 16.0 34.0 4 31.52131795817644 35.0 32.0 37.0 16.0 37.0 5 31.879776423283452 35.0 32.0 37.0 17.0 37.0 6 31.80636720193603 36.0 32.0 37.0 17.0 37.0 7 31.679092988363042 36.0 32.0 37.0 15.0 37.0 8 31.547402759966687 36.0 32.0 37.0 11.0 37.0 9 32.54612376762872 38.0 32.0 39.0 10.0 39.0 10 32.55334981888038 38.0 32.0 39.0 10.0 39.0 11 32.639012502107526 38.0 32.0 39.0 10.0 39.0 12 32.629188444448985 38.0 32.0 39.0 10.0 39.0 13 32.59039546579334 38.0 32.0 39.0 10.0 39.0 14 33.389109150330135 39.0 32.0 41.0 10.0 41.0 15 33.49533448800285 39.0 32.0 41.0 10.0 41.0 16 33.4179571546082 39.0 32.0 41.0 10.0 41.0 17 33.31131031147125 38.0 32.0 41.0 10.0 41.0 18 33.168093824561765 38.0 32.0 41.0 10.0 41.0 19 33.54527990517404 39.0 32.0 41.0 10.0 41.0 20 33.57379070508072 39.0 32.0 41.0 10.0 41.0 21 33.59313652860021 39.0 32.0 41.0 10.0 41.0 22 33.491185000877074 39.0 32.0 41.0 10.0 41.0 23 33.23956922993081 39.0 32.0 41.0 10.0 41.0 24 33.41723165429399 39.0 32.0 41.0 10.0 41.0 25 33.34010466959932 39.0 32.0 41.0 10.0 41.0 26 33.36055492258775 39.0 32.0 41.0 10.0 41.0 27 33.361994853375705 39.0 32.0 41.0 9.0 41.0 28 33.25195127226528 39.0 32.0 41.0 8.0 41.0 29 33.22810087519862 39.0 32.0 41.0 8.0 41.0 30 32.97203758295994 38.0 32.0 41.0 2.0 41.0 31 32.887578106239815 38.0 31.0 40.0 2.0 41.0 32 32.86379072211124 38.0 31.0 40.0 2.0 41.0 33 32.83533101377599 38.0 31.0 40.0 2.0 41.0 34 32.845562100953536 38.0 31.0 40.0 2.0 41.0 35 32.78582157801427 38.0 31.0 40.0 2.0 41.0 36 32.629191850553745 38.0 31.0 40.0 2.0 41.0 37 32.57334705993552 38.0 31.0 40.0 2.0 41.0 38 32.437258698765795 38.0 31.0 40.0 2.0 41.0 39 32.43144107183305 38.0 31.0 40.0 2.0 41.0 40 32.40893268004244 38.0 31.0 40.0 2.0 41.0 41 32.33893382108754 38.0 31.0 40.0 2.0 41.0 42 32.15214388748955 38.0 30.0 40.0 2.0 41.0 43 32.093758994245384 38.0 30.0 40.0 2.0 41.0 44 32.04233958523862 38.0 30.0 40.0 2.0 41.0 45 31.956527884928157 38.0 30.0 40.0 2.0 41.0 46 31.804983471876646 38.0 30.0 40.0 2.0 41.0 47 31.60434857394909 38.0 30.0 40.0 2.0 41.0 48 31.426012592369304 38.0 30.0 40.0 2.0 41.0 49 31.354684501031198 38.0 30.0 40.0 2.0 41.0 50 31.16906371289262 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1384479075403036 1101 2 1.134755391832904 1101 3 1.2439257831268726 1101 4 1.162180325151688 1101 5 1.0944703660054458 1101 6 1.1893287263572034 1101 7 1.1488500558989614 1101 8 1.2618957890428035 1101 9 1.4247442635506147 1101 10 1.5958347824240207 1101 11 1.3101632715994995 1101 12 1.4938335249151393 1101 13 1.489796710149669 1101 14 1.7667484216660583 1101 15 1.6612731176387499 1101 16 1.3682672954808197 1101 17 1.618361653145854 1101 18 1.7685234640892986 1101 19 1.4810576485826985 1101 20 1.4667738304994202 1101 21 1.4834713243739728 1101 22 1.5534519806549625 1101 23 1.3287173578919464 1101 24 1.4119769786379806 1101 25 1.3130012612361668 1101 26 1.3622062602341316 1101 27 1.4921149240158371 1101 28 1.6530442399867695 1101 29 1.6022193631353545 1101 30 1.8471080341496133 1101 31 1.5803895795179699 1101 32 1.5062266072601176 1101 33 1.4435451536842336 1101 34 1.4119440697043615 1101 35 1.2113234070642633 1101 36 1.311371201134321 1101 37 1.4030741181241595 1101 38 1.4579684135915016 1101 39 1.4728742652634637 1101 40 1.4270362699872408 1101 41 1.500279015585967 1101 42 1.5761138951660598 1101 43 1.5701467100952229 1101 44 1.3673074332537212 1101 45 1.3145918536949175 1101 46 1.3124217284091948 1101 47 1.3667493725534925 1101 48 1.3400783317381446 1101 49 1.3775864781183529 1101 50 1.3211968709093114 1102 1 0.449865532892634 1102 2 0.26629520101784365 1102 3 0.41110790718739665 1102 4 0.29470729581346333 1102 5 0.21563377015767315 1102 6 0.39300798204467924 1102 7 0.4479719161017073 1102 8 0.3211572982427491 1102 9 0.6063100173641089 1102 10 0.5789214157875477 1102 11 0.42421477394717044 1102 12 0.5022688739743515 1102 13 0.5668596697176369 1102 14 0.8548892856312307 1102 15 0.9009186796608759 1102 16 0.9144109473635638 1102 17 0.9398045722510489 1102 18 0.9843683097865608 1102 19 0.7261709280741471 1102 20 0.5655312003107227 1102 21 0.5615633907415969 1102 22 0.6337617251681706 1102 23 0.8832027050527742 1102 24 0.7013675239452581 1102 25 0.5952202411331484 1102 26 0.4760798565574049 1102 27 0.5239026083628175 1102 28 0.6587516966718923 1102 29 0.4721411314923145 1102 30 0.8127924209071864 1102 31 0.7029552592039536 1102 32 0.6199461630012877 1102 33 0.44191868763191877 1102 34 0.6235719640672315 1102 35 0.6492398093126752 1102 36 0.8061719698908405 1102 37 0.7569454725422489 1102 38 0.6180165783932239 1102 39 0.7278841097584134 1102 40 0.7329749683443154 1102 41 0.7583132624956619 1102 42 0.719817124775119 1102 43 0.768421527895228 1102 44 0.930663492996878 1102 45 0.7805468962639956 1102 46 0.7990196553700919 1102 47 0.9396396764943589 1102 48 1.0215686924498435 1102 49 1.0318434763881044 1102 50 0.9779011618073596 1103 1 0.7169191676056244 1103 2 0.8132105083590986 1103 3 0.7900702269996884 1103 4 0.6938055182976015 1103 5 0.6366853660143121 1103 6 0.6867122312082472 1103 7 0.6817319614752222 1103 8 0.7792639309214451 1103 9 0.8746497972365006 1103 10 0.8414092351846563 1103 11 0.9347905066370146 1103 12 0.8163739857688341 1103 13 0.8000237308861387 1103 14 0.9668953101319389 1103 15 0.871691909427895 1103 16 0.9348075931251003 1103 17 1.0901134755030597 1103 18 0.9728864772897481 1103 19 0.8185519939089687 1103 20 0.9289410030561172 1103 21 0.8877435079916509 1103 22 0.8978986918967209 1103 23 1.0090667679431604 1103 24 0.9678870218707374 1103 25 0.7280122690010344 1103 26 0.8107372868745202 1103 27 0.8035369214506076 1103 28 0.8683181568173808 1103 29 0.8335275397492268 1103 30 0.909336942438344 1103 31 0.7961392872489483 1103 32 0.688303021194649 1103 33 0.647213107694661 1103 34 0.6573290576321824 1103 35 0.567327473107305 1103 36 0.7625962473917696 1103 37 0.850494611193966 1103 38 0.7541054953008697 1103 39 0.8318668374614475 1103 40 0.8731141882867348 1103 41 0.7601296789789842 1103 42 0.857020700820911 1103 43 0.6352974215640543 1103 44 0.6415904206245031 1103 45 0.7578288639114668 1103 46 0.5339809814068381 1103 47 0.5369778479824916 1103 48 0.6426685714608276 1103 49 0.46730481771099974 1103 50 0.7034978751793695 1104 1 0.6132520875750735 1104 2 0.6390104786454103 1104 3 0.6604417390524304 1104 4 0.7049164834230979 1104 5 0.6340872712613432 1104 6 0.7440700914723877 1104 7 0.8772375809668631 1104 8 0.6850988299504976 1104 9 0.8551401674077326 1104 10 0.8962121226004385 1104 11 0.8794061472887051 1104 12 0.8355002349143987 1104 13 0.8519580009754719 1104 14 1.1586351745472427 1104 15 0.9016417566314914 1104 16 1.0250849848832289 1104 17 1.046486236069697 1104 18 1.2290639427930685 1104 19 1.0574854140251304 1104 20 0.8693152001211999 1104 21 0.8246676157977575 1104 22 1.0162097012221665 1104 23 1.0940630739456267 1104 24 0.8566951694850999 1104 25 0.9898420729075426 1104 26 0.9247738374051337 1104 27 0.8430298482791372 1104 28 0.934429426828224 1104 29 0.9358487447099932 1104 30 1.1039194836593467 1104 31 1.018392952526618 1104 32 0.937173008315348 1104 33 0.9421406578415628 1104 34 0.7865660795391207 1104 35 0.9713180684894169 1104 36 0.9312601657107962 1104 37 1.083369403711913 1104 38 0.9249349417672121 1104 39 0.8309740792799971 1104 40 1.0421995558453858 1104 41 1.038984549850774 1104 42 1.0257435748689048 1104 43 1.0608206705031051 1104 44 0.8208495256630641 1104 45 0.7143966837185012 1104 46 0.8709864144299253 1104 47 0.639066262036323 1104 48 0.6044140518304282 1104 49 0.7570752575780091 1104 50 0.7325226227730504 1105 1 0.5793867025948529 1105 2 0.6339892534939473 1105 3 0.5696730608210174 1105 4 0.6483478076776024 1105 5 0.42735024006112354 1105 6 0.31712432175072536 1105 7 0.39886538555546736 1105 8 0.6083742741282521 1105 9 0.5914789089188162 1105 10 0.7617426598005466 1105 11 0.7540567287643043 1105 12 0.7362598133813805 1105 13 0.7157961520812748 1105 14 1.0564809966694142 1105 15 0.8654965241295685 1105 16 1.037772647081134 1105 17 0.8771979083002392 1105 18 0.9926895479993618 1105 19 0.845041567945259 1105 20 0.7569439035650447 1105 21 0.8264977086075049 1105 22 0.8562085629338796 1105 23 0.9749529798798378 1105 24 1.0014363757174252 1105 25 0.9695435797003071 1105 26 0.9959005007616071 1105 27 0.9744982205465149 1105 28 1.1316709094552593 1105 29 0.9450531793241623 1105 30 1.3171480290512108 1105 31 1.2574105966492937 1105 32 1.0512847738331956 1105 33 1.0129912535784769 1105 34 0.8663123328370546 1105 35 1.0992963441920054 1105 36 1.0029474687591176 1105 37 0.936696705425689 1105 38 1.0086599705678694 1105 39 1.180365127754719 1105 40 1.085963469620701 1105 41 1.2838931629642474 1105 42 1.0901062544518751 1105 43 1.1493373042313735 1105 44 0.8598754779818663 1105 45 0.8572613497453752 1105 46 1.1449619386149585 1105 47 1.0696310034911463 1105 48 1.2365032491474643 1105 49 1.0887495684393116 1105 50 1.1716434978681534 1106 1 -0.025017719369433422 1106 2 0.07906814520909933 1106 3 0.14122396441296914 1106 4 0.11037323104851993 1106 5 0.01976968344977692 1106 6 -0.06755148641424924 1106 7 -0.03935283441873594 1106 8 0.08349828859092057 1106 9 -1.334527906597689 1106 10 -0.8862956864133977 1106 11 -0.2775145758970794 1106 12 0.013546731033180492 1106 13 -0.015430478461205155 1106 14 0.10123340630210009 1106 15 0.14164254121058661 1106 16 0.18965633499674794 1106 17 0.2651248932625734 1106 18 -1.3891804634031466 1106 19 -0.379682059286921 1106 20 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0.21709192117997844 1107 7 0.46851228164310044 1107 8 0.30402512059315967 1107 9 0.5249275135134681 1107 10 0.6069040707207876 1107 11 0.6059798895646011 1107 12 0.6168767950189817 1107 13 0.4578682931982243 1107 14 0.8920418692417087 1107 15 0.7023029494119797 1107 16 0.5062132449962817 1107 17 0.793428101893646 1107 18 0.7080872961596967 1107 19 0.7190102017004278 1107 20 0.6565663122982457 1107 21 0.554785276134659 1107 22 0.6885468248924909 1107 23 0.8847753375198337 1107 24 0.5978386661885438 1107 25 0.6294590889812 1107 26 0.7113007412735399 1107 27 0.49547945936987503 1107 28 0.555674124588819 1107 29 0.7196330623082545 1107 30 0.8526154384386473 1107 31 0.8428646096469024 1107 32 0.7541241853889815 1107 33 0.7005936736518734 1107 34 0.5907448555330319 1107 35 0.3710810548611363 1107 36 0.3878095482526689 1107 37 0.33448910499949847 1107 38 0.3146652917385282 1107 39 0.4287572494313281 1107 40 0.43376864249643887 1107 41 0.32420881929388656 1107 42 0.3991472598665027 1107 43 0.36695386840008126 1107 44 0.3265764144829788 1107 45 0.431528442604165 1107 46 0.6655774248415973 1107 47 0.6259176488482083 1107 48 0.4090612118992709 1107 49 0.31563643436948396 1107 50 0.5394261149619446 1108 1 -0.3067615099237955 1108 2 -0.19693904632672599 1108 3 -0.2648788005003695 1108 4 -0.3665275684293725 1108 5 -0.2911741899390279 1108 6 -0.15676041747340008 1108 7 -0.3293970127889061 1108 8 -0.14920060820489311 1108 9 -0.15180390845519298 1108 10 -0.3252906284920414 1108 11 -0.30336268764827423 1108 12 -0.2303759191670025 1108 13 -0.21138978917822016 1108 14 -0.25215904070268635 1108 15 -0.23943292598822552 1108 16 -0.14974713149923247 1108 17 -0.33186859293796545 1108 18 -0.12475315062781789 1108 19 -0.27179647122511597 1108 20 -0.29304190608841196 1108 21 -0.2528589789386402 1108 22 -0.1718092089054437 1108 23 -0.6014262464574429 1108 24 -0.2775182101504967 1108 25 -0.1929302372266406 1108 26 -0.27705834974497634 1108 27 -0.2632239902341311 1108 28 -0.12478193087445533 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-1.4768639425650605 2216 20 -1.528137657353568 2216 21 -1.6618337058301655 2216 22 -1.895824015978743 2216 23 -3.2631914877416364 2216 24 -2.2445074742727904 2216 25 -1.7689645265021134 2216 26 -1.9462016960921424 2216 27 -1.9613633617580355 2216 28 -2.2445633691351325 2216 29 -2.0738565812120697 2216 30 -1.6614979050426264 2216 31 -1.5653167037943376 2216 32 -1.7509986658108367 2216 33 -1.7895784960153414 2216 34 -1.9272834152523224 2216 35 -2.0920662417217706 2216 36 -1.986439384486662 2216 37 -1.9099955415891188 2216 38 -1.9916221819286228 2216 39 -2.2941765684529614 2216 40 -2.272590823116115 2216 41 -1.9383925605074595 2216 42 -2.21595298426854 2216 43 -2.297818692839563 2216 44 -2.1808839168924905 2216 45 -2.2825285824548764 2216 46 -2.360698572618759 2216 47 -2.303386792071908 2216 48 -2.2696780524014883 2216 49 -2.494013408496805 2216 50 -2.825684091158166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73137.0 3 3334.0 4 6942.0 5 5027.0 6 4035.0 7 3455.0 8 3324.0 9 2994.0 10 2758.0 11 2834.0 12 3064.0 13 3271.0 14 3213.0 15 3091.0 16 3237.0 17 3131.0 18 3515.0 19 4262.0 20 6159.0 21 9842.0 22 13481.0 23 16189.0 24 16907.0 25 15872.0 26 14755.0 27 14894.0 28 16383.0 29 18604.0 30 20738.0 31 24521.0 32 30176.0 33 37109.0 34 46826.0 35 60920.0 36 79123.0 37 104499.0 38 166169.0 39 326571.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.169013544431614 24.414232091653922 14.892739520001363 21.5240148439131 2 13.046390461143579 26.19487095581155 40.86643589887803 19.89230268416684 3 13.87103893680279 35.10643646406486 30.66840957566017 20.35411502347218 4 10.024383612780388 21.50556237849742 44.13186753246532 24.338186476256872 5 9.119399830217818 43.53123071625579 36.9820876389708 10.367281814555582 6 22.118566149058534 48.01544729233541 18.909639986400112 10.95634657220595 7 22.913651013813148 36.624644192012354 27.086391585167718 13.375313209006787 8 21.543230547297508 40.036196226492265 22.62604986524404 15.794523360966187 9 24.971477309230274 20.177620317187657 23.01847179725756 31.832430576324505 10 13.441552022687919 31.189326179730852 36.830628008126205 18.53849378945503 11 25.585469368140412 32.6778178149691 27.66346492210925 14.073247894781238 12 18.51551694605436 34.50840634397964 30.35581132361676 16.620265386349246 13 23.542875841611327 28.43238093452624 29.506609753956774 18.518133469905656 14 19.5145500345983 28.107029761356934 30.883841740891103 21.494578463153662 15 20.1498147131719 36.91750559951454 23.78233996638587 19.150339720927693 16 19.77613943646248 33.21322744243482 28.778286173022366 18.232346948080334 17 15.63321710239772 36.08923671988401 29.406455121249802 18.87109105646846 18 16.308522759715053 34.639695724686405 31.01752071524811 18.03426080035043 19 18.91634170950193 32.775452326738744 31.801773857082477 16.506432106676847 20 17.043193078061865 34.385027695082215 32.009361525867355 16.56241770098857 21 18.98306847101458 32.86937682495905 30.78263789573806 17.36491680828832 22 18.867847345262838 31.159807498425508 31.36885936766284 18.603485788648815 23 18.851309950819854 33.59640578435558 30.565235065077832 16.98704919974673 24 18.40977337255316 33.89995875782664 29.97249385627955 17.71777401334065 25 18.99200758163295 32.53011432107056 31.66081737775725 16.81706071953924 26 17.338088289443178 32.833489028597924 33.00165696128302 16.826765720675876 27 17.17598467912326 34.489274278296875 30.251827962304645 18.082913080275222 28 18.908562694520477 29.890280007194754 32.96859455943642 18.232562738848344 29 17.436410176417493 32.499789035470165 32.56337672809783 17.50042406001451 30 17.70563087863798 33.33517995152201 30.343914171459353 18.615274998380656 31 19.759535980119278 30.913381478407153 32.29194186456769 17.035140676905876 32 17.306187709127727 33.38322000876141 32.11145069656685 17.199141585544 33 18.169785913956503 33.57077111883821 30.790976341446786 17.468466625758506 34 19.28457850667821 30.080748654899203 33.15143014423298 17.483242694189606 35 17.3234419999267 34.55048781922179 31.274381372610016 16.85168880824149 36 18.16842067579996 32.53976033419222 30.18751006846986 19.10430892153796 37 20.471365661297995 30.021686093792354 31.373230972242823 18.13371727266683 38 17.40670444482891 33.0930224035468 32.84407516655431 16.656197985069984 39 17.808304384464833 33.925602899061964 29.763356313494732 18.502736402978474 40 17.56077484357332 31.839550493245728 33.54011280274517 17.05956186043579 41 17.811716108626896 32.210011315301564 32.45634065874141 17.521931917330132 42 18.10000826542759 32.7564008067739 30.399560995021147 18.744029932777362 43 17.853507021027728 31.519273217354115 32.05214379241986 18.57507596919829 44 16.861779614090384 33.379222217489456 31.681219917365933 18.077778251054223 45 18.243490765841365 32.417016955274946 30.76544947409852 18.57404280478517 46 18.303535677957004 30.89019820992566 31.479989027147585 19.32627708496975 47 18.18631739360429 33.310390708918575 31.374691956934875 17.12859994054226 48 17.379147238411843 33.48674306482655 30.108207534229653 19.02590216253196 49 18.603789242654155 31.448311352817736 31.987294782016733 17.960604622511376 50 17.575727629296683 31.649701996571856 31.59330454962123 19.181265824510227 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12417.0 1 9386.5 2 6356.0 3 5853.5 4 5351.0 5 4458.0 6 3565.0 7 2890.0 8 2215.0 9 2325.0 10 2435.0 11 2773.0 12 3111.0 13 5018.5 14 6926.0 15 9533.5 16 12141.0 17 12600.0 18 13059.0 19 11870.5 20 10682.0 21 11371.5 22 12061.0 23 15552.0 24 19043.0 25 26885.5 26 34728.0 27 43471.0 28 52214.0 29 59653.0 30 67092.0 31 73790.5 32 80489.0 33 97036.5 34 113584.0 35 111025.0 36 108466.0 37 112856.0 38 117246.0 39 120675.0 40 124104.0 41 118330.0 42 112556.0 43 90421.5 44 68287.0 45 60702.5 46 53118.0 47 47056.5 48 40995.0 49 35085.5 50 29176.0 51 24485.5 52 19795.0 53 15640.5 54 11486.0 55 9469.5 56 7453.0 57 6143.0 58 4833.0 59 4332.0 60 3831.0 61 3552.5 62 3274.0 63 2896.0 64 2518.0 65 2274.0 66 2030.0 67 1773.0 68 1516.0 69 1438.0 70 1360.0 71 1269.0 72 1178.0 73 1136.5 74 1095.0 75 950.5 76 806.0 77 719.0 78 632.0 79 524.5 80 417.0 81 355.5 82 294.0 83 235.0 84 176.0 85 128.0 86 80.0 87 73.0 88 66.0 89 49.0 90 32.0 91 21.5 92 11.0 93 15.5 94 20.0 95 14.0 96 8.0 97 9.5 98 11.0 99 17.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0695696897549478 2 0.07561552570672417 3 0.0719539630880427 4 0.08796265546739421 5 0.09315696522877955 6 0.07008060546918242 7 0.053220386899439864 8 0.06463083785067977 9 0.06258717499374128 10 0.0744233890401767 11 0.062246564517584875 12 0.07689281499231072 13 0.07399762594498119 14 0.07612644142095878 15 0.08906963951490256 16 0.0811504459442659 17 0.09562639118091355 18 0.0809801407061877 19 0.0744233890401767 20 0.08932509737201987 21 0.08923994475298076 22 0.08251288784889156 23 0.07842556213501459 24 0.06863301094551766 25 0.08515261903910377 26 0.09622245951418727 27 0.09034692880048911 28 0.10942111546524837 29 0.10039493784710336 30 0.08906963951490256 31 0.08208712475369605 32 0.08821811332451153 33 0.085322924277182 34 0.07186881046900359 35 0.09187967594319298 36 0.09749974879977384 37 0.10729229998927077 38 0.08694082403892496 39 0.0630980907079759 40 0.06888846880263497 41 0.06216141189854576 42 0.06803694261224393 43 0.04521604070976411 44 0.04359814094802114 45 0.04836668761421095 46 0.047344856185741704 47 0.03567894737738449 48 0.05100641880442317 49 0.05713740737523864 50 0.04683394047150708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1174362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.7482218898986 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4153746261146 21.138786440558984 2 5.9182958705838296 2.929345988305584 3 2.136457412224273 1.586205662881346 4 1.0788114877451065 1.0679466430435005 5 0.6771957179077878 0.8379694944835555 6 0.48979784492058365 0.7272975448373248 7 0.36686330824547914 0.6355450189004969 8 0.28051029063222976 0.5553704731973091 9 0.2232613525745596 0.4972789343658676 >10 2.258156644081797 12.248174837558977 >50 0.5229325472371336 9.16815602570331 >100 0.5519455143835433 28.706328659812424 >500 0.05872504522890139 9.63452257361977 >1k 0.020274122757596904 7.435307042946958 >5k 0.0010486615219446676 1.8466074548714444 >10k+ 3.4955384064822253E-4 0.9851572049131457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11388 0.969718025617314 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 8338 0.7100025375480473 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 6655 0.5666906797052357 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6353 0.5409745887554264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3415 0.2907961940185394 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 3323 0.28296215306694184 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.24115221711874193 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 2685 0.2286347821199937 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2618 0.2229295566443737 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2336 0.19891651807534644 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2271 0.1933815978378047 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2142 0.1823969099817603 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1927 0.164089096888353 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1853 0.15778780307945933 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1824 0.1553183771273253 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1704 0.14510006284263285 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1631 0.13888392165277827 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1612 0.13726602189103532 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1590 0.13539266427217503 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1584 0.13488174855794038 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1578 0.13437083284370577 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1551 0.13207171212964997 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1492 0.12704770760634285 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1485 0.12645163927306913 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1442 0.12279007665438767 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1440 0.12261977141630946 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1417 0.12066126117841007 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1403 0.1194691245118626 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1382 0.11768091951204145 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1381 0.11759576689300232 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 1368 0.11648878284549397 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1353 0.11521149355990742 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 1274 0.10848443665581822 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGT 1262 0.10746260522734898 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1256 0.10695168951311436 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1192 0.10150192189461171 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1189 0.1012464640374944 No Hit ATATTATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1186 0.10099100618037708 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1185 0.10090585356133798 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1182 0.10065039570422067 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.515261903910379E-5 0.0 9 0.0 0.0 0.0 8.515261903910379E-5 0.0 10 0.0 0.0 0.0 8.515261903910379E-5 0.0 11 0.0 0.0 0.0 8.515261903910379E-5 0.0 12 0.0 0.0 0.0 8.515261903910379E-5 0.0 13 0.0 0.0 0.0 8.515261903910379E-5 0.0 14 0.0 0.0 0.0 1.7030523807820758E-4 0.0 15 0.0 0.0 0.0 2.554578571173114E-4 0.0 16 0.0 0.0 0.0 2.554578571173114E-4 0.0 17 0.0 0.0 0.0 3.4061047615641517E-4 0.0 18 0.0 0.0 0.0 4.257630951955189E-4 0.0 19 0.0 0.0 0.0 5.109157142346227E-4 0.0 20 0.0 0.0 0.0 5.960683332737265E-4 0.0 21 0.0 0.0 0.0 0.0010218314284692455 0.0 22 0.0 0.0 0.0 0.0012772892855865568 0.0 23 0.0 0.0 0.0 0.0022139680950166983 0.0 24 0.0 0.0 0.0 0.0037467152377205666 0.0 25 0.0 0.0 0.0 0.0040021730948378775 0.0 26 0.0 0.0 0.0 0.005875530713698161 0.0 27 0.0 0.0 0.0 0.006727056904089199 0.0 28 0.0 0.0 0.0 0.00919648285622321 0.0 29 0.0 0.0 0.0 0.01328380857010019 0.0 30 0.0 0.0 0.0 0.020181170712267597 0.0 31 0.0 0.0 0.0 0.03346497928236779 0.0 32 0.0 0.0 0.0 0.04521604070976411 0.0 33 0.0 0.0 0.0 0.058414696660825195 0.0 34 0.0 0.0 0.0 0.07280548927843374 0.0 35 0.0 0.0 0.0 0.09034692880048911 0.0 36 0.0 0.0 0.0 0.11180538879834327 0.0 37 0.0 0.0 0.0 0.139479989986052 0.0 38 0.0 0.0 0.0 0.1689427961735819 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATGC 20 7.8644615E-4 43.99101 38 CGCTTCA 25 0.002354632 35.19431 8 GATTGCC 50 2.3612138E-6 30.80027 9 AGACGCT 30 0.0057470934 29.331089 5 CTAGGTC 30 0.0057482906 29.32984 18 GAACTCG 40 7.0193975E-4 27.508444 31 TGGCATG 40 7.0228963E-4 27.5061 37 TACGCCG 40 7.035156E-4 27.497894 5 CGCAGAA 95 9.622454E-10 25.477165 2 AATGCCG 70 1.131295E-6 25.14522 29 GACATAT 195 0.0 24.82602 1 CGTTGTT 45 0.0013981181 24.444658 24 GTCGTTG 45 0.0013984652 24.443617 22 AGTTTAC 100 1.7444108E-9 24.201242 14 GGTATCA 1585 0.0 24.156778 1 TCGGCAA 55 1.5902628E-4 24.007372 35 GGTCATT 55 1.5934849E-4 24.00021 17 CGTTAAT 55 1.5934849E-4 24.00021 26 GTATCAA 3765 0.0 23.962818 1 GTATGGT 120 2.3646862E-11 23.838621 1 >>END_MODULE