##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174362
Sequences flagged as poor quality	0
Sequence length	50
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.387029723373203	31.0	30.0	34.0	26.0	34.0
2	30.322577706022503	31.0	30.0	34.0	26.0	34.0
3	30.50269763497116	31.0	30.0	34.0	26.0	34.0
4	33.95947331402072	37.0	35.0	37.0	30.0	37.0
5	33.666819941380936	37.0	35.0	37.0	28.0	37.0
6	33.58741086649602	37.0	35.0	37.0	28.0	37.0
7	33.411896842711194	37.0	35.0	37.0	27.0	37.0
8	33.36700438195378	37.0	35.0	37.0	27.0	37.0
9	34.71604070976411	39.0	35.0	39.0	27.0	39.0
10	34.625022778325594	39.0	35.0	39.0	27.0	39.0
11	34.60678053274884	39.0	35.0	39.0	27.0	39.0
12	34.56839884124316	39.0	35.0	39.0	27.0	39.0
13	34.5170501089102	39.0	35.0	39.0	26.0	39.0
14	35.62904198194424	40.0	36.0	41.0	26.0	41.0
15	35.63686069542441	40.0	36.0	41.0	27.0	41.0
16	35.609308714008115	40.0	36.0	41.0	27.0	41.0
17	35.65959729623404	40.0	36.0	41.0	27.0	41.0
18	35.69413775309487	40.0	36.0	41.0	27.0	41.0
19	35.80325827981491	40.0	36.0	41.0	27.0	41.0
20	35.811352036254576	40.0	36.0	41.0	27.0	41.0
21	35.77486328747013	40.0	36.0	41.0	27.0	41.0
22	35.75898913622886	40.0	36.0	41.0	27.0	41.0
23	35.68314199539835	40.0	35.0	41.0	27.0	41.0
24	35.6713602790281	40.0	35.0	41.0	27.0	41.0
25	35.67694458778469	40.0	35.0	41.0	27.0	41.0
26	35.57222730299516	40.0	35.0	41.0	27.0	41.0
27	35.52401474162141	40.0	35.0	41.0	27.0	41.0
28	35.466516287141445	40.0	35.0	41.0	26.0	41.0
29	35.379036447024	39.0	35.0	41.0	26.0	41.0
30	35.19245939497361	39.0	35.0	41.0	25.0	41.0
31	35.05037118026639	39.0	35.0	41.0	25.0	41.0
32	34.89725995902456	39.0	35.0	41.0	24.0	41.0
33	34.771062926082415	39.0	34.0	41.0	24.0	41.0
34	34.739977962502195	39.0	34.0	41.0	24.0	41.0
35	34.66644612138335	39.0	34.0	41.0	24.0	41.0
36	34.497003479336016	39.0	34.0	41.0	22.0	41.0
37	34.42800346060244	39.0	34.0	41.0	21.0	41.0
38	34.28830547991164	39.0	34.0	41.0	19.0	41.0
39	34.231512089117324	39.0	34.0	41.0	19.0	41.0
40	34.15040932863972	39.0	34.0	41.0	18.0	41.0
41	34.115358807590844	39.0	34.0	41.0	16.0	41.0
42	33.96199383154428	39.0	33.0	41.0	15.0	41.0
43	33.98467167704677	39.0	34.0	41.0	15.0	41.0
44	33.86800407370129	39.0	33.0	41.0	9.0	41.0
45	33.730818946798344	39.0	33.0	41.0	2.0	41.0
46	33.52088964050267	39.0	33.0	41.0	2.0	41.0
47	33.29204367988746	39.0	33.0	41.0	2.0	41.0
48	33.09488045423813	39.0	33.0	41.0	2.0	41.0
49	32.94722155519337	39.0	33.0	41.0	2.0	41.0
50	32.83761140091386	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7605780836155951
1101	2	0.9917710677253133
1101	3	0.9101113665851592
1101	4	0.8300022393243935
1101	5	0.9369852089113593
1101	6	0.9390265070361679
1101	7	0.8327036524300411
1101	8	0.8462448500699864
1101	9	0.8438775288616469
1101	10	0.9464139714429152
1101	11	1.0649943088575995
1101	12	1.0126084435445506
1101	13	0.9297093054231738
1101	14	1.2948398951164606
1101	15	1.101079301138327
1101	16	0.9762484832686908
1101	17	1.2481821718006074
1101	18	1.03931009264371
1101	19	1.1580248228688248
1101	20	1.2014988910373958
1101	21	1.0988067505396444
1101	22	1.1466930388930479
1101	23	1.2562293039183743
1101	24	1.118151974304574
1101	25	1.1813753106229683
1101	26	1.2791633121195431
1101	27	1.2579308124453732
1101	28	1.313940825707114
1101	29	1.3268079715088419
1101	30	1.3077436810772056
1101	31	1.464726438038646
1101	32	1.5098897639854698
1101	33	1.5048157720638855
1101	34	1.3307680129550832
1101	35	1.4499433429956952
1101	36	1.4441771776223007
1101	37	1.4735719239354168
1101	38	1.433522555503842
1101	39	1.3498591323340747
1101	40	1.4877784318365102
1101	41	1.47106241235781
1101	42	1.5123836365075363
1101	43	1.5408560932508593
1101	44	1.4644363543834586
1101	45	1.5180821094155448
1101	46	1.541052717365389
1101	47	1.6484840324454666
1101	48	1.674076057360061
1101	49	1.5712624679772347
1101	50	1.6958426853981337
1102	1	0.5508847364692322
1102	2	0.6672881975081175
1102	3	0.5732745140030815
1102	4	0.54471040293285
1102	5	0.6749383133172486
1102	6	0.647751676580242
1102	7	0.5041737817554264
1102	8	0.5915897328310038
1102	9	0.606300666769549
1102	10	0.7596238553520038
1102	11	0.776629977569101
1102	12	0.6730473145644922
1102	13	0.7194243845859276
1102	14	0.7081944180697732
1102	15	0.6803340846227215
1102	16	0.8859665998513719
1102	17	0.7953947062201792
1102	18	0.7782221539128074
1102	19	0.6877808710408573
1102	20	0.6750268723644979
1102	21	0.7711577316443652
1102	22	0.7147064758602539
1102	23	0.7791453716890047
1102	24	0.838798293873694
1102	25	0.8414945293551739
1102	26	0.7587028537222551
1102	27	0.662604461561898
1102	28	0.7947557220822787
1102	29	0.8931399795869339
1102	30	0.8431399287787968
1102	31	0.8413440901491995
1102	32	0.969379441277681
1102	33	0.9626149805267161
1102	34	1.012510768798954
1102	35	1.0032950816474013
1102	36	0.8405149170225243
1102	37	0.88826218846215
1102	38	1.0163622176993528
1102	39	0.9861751035581321
1102	40	1.0963660134897708
1102	41	1.0886877646894817
1102	42	1.080188431653454
1102	43	0.9274968695286105
1102	44	0.8802486562957696
1102	45	0.9630048269662339
1102	46	1.00578013604661
1102	47	1.0574874513508732
1102	48	0.9142701629574077
1102	49	0.9376950001910629
1102	50	0.8896976956042266
1103	1	0.5584684246345546
1103	2	0.8146526717189744
1103	3	0.5398452820320401
1103	4	0.6725342558653153
1103	5	0.5930973124854688
1103	6	0.6359374142143608
1103	7	0.7358626414542115
1103	8	0.6474274086612795
1103	9	0.7458312518988279
1103	10	0.7923453560795934
1103	11	0.9225572021346196
1103	12	0.753524509559206
1103	13	0.824507715195864
1103	14	0.8791888226355127
1103	15	0.8649103226340742
1103	16	0.9853012832857146
1103	17	0.8977279351859977
1103	18	0.8068768355338989
1103	19	0.89006655480447
1103	20	0.8208739764118818
1103	21	0.9235251699629856
1103	22	0.8762593098951683
1103	23	1.0192229901979672
1103	24	1.0374708233944219
1103	25	0.9753727524929232
1103	26	1.0049012581291592
1103	27	0.9652878312481619
1103	28	1.0240553738742904
1103	29	0.9075609700381833
1103	30	1.1051255893144898
1103	31	0.9397612530886761
1103	32	1.033918978469238
1103	33	0.8982082747204743
1103	34	1.0063854146258535
1103	35	1.045922359942864
1103	36	1.060055678863229
1103	37	1.1017823150525672
1103	38	0.772204426664203
1103	39	0.9217040904939182
1103	40	0.8817006554582392
1103	41	0.8863036873071195
1103	42	0.7844948743621885
1103	43	0.8974211029281136
1103	44	0.9015867181452961
1103	45	0.9305293102855217
1103	46	0.9865480490441598
1103	47	1.0597195808734199
1103	48	1.0763620534607696
1103	49	1.205394167324222
1103	50	1.0133970988820664
1104	1	0.6480211662612412
1104	2	0.7865732371279996
1104	3	0.6572719554230311
1104	4	0.6315084800837241
1104	5	0.6596805652178972
1104	6	0.5471955665283446
1104	7	0.6222310325591067
1104	8	0.5878352947143384
1104	9	0.7632979814965282
1104	10	0.8378686117564342
1104	11	0.8064100365070885
1104	12	0.7970395646625974
1104	13	0.7342855733780311
1104	14	0.8840164546129685
1104	15	0.8467138895567388
1104	16	0.8539101874873722
1104	17	0.9289950218195884
1104	18	0.832466317023318
1104	19	0.8758807957460917
1104	20	0.7333169704331013
1104	21	0.7977405955441768
1104	22	0.7085562015133178
1104	23	0.7986045883968202
1104	24	0.8637629897951129
1104	25	0.6170182708973897
1104	26	0.674291342584084
1104	27	0.7356434757875618
1104	28	0.7757476559629666
1104	29	0.9549630605746486
1104	30	1.0312429435565562
1104	31	0.9823997207248496
1104	32	0.809052058394002
1104	33	0.9290289822633397
1104	34	0.8339560781203659
1104	35	0.9347850837177489
1104	36	0.865027432331253
1104	37	0.8213693284867247
1104	38	0.982913212651205
1104	39	0.9577546765307119
1104	40	0.8774652758894632
1104	41	0.879717490157141
1104	42	1.0195035273787454
1104	43	1.0038062105088414
1104	44	1.0909555515866032
1104	45	1.0379112750205692
1104	46	0.9352451105895625
1104	47	1.0847697016652873
1104	48	1.0994942601669706
1104	49	1.0785477160043015
1104	50	1.0579336041009952
1105	1	0.43056950589768306
1105	2	0.5577674781742807
1105	3	0.4241299068573703
1105	4	0.4635703977331431
1105	5	0.5589152298906015
1105	6	0.4909327239869725
1105	7	0.3871018859024318
1105	8	0.39080936725470394
1105	9	0.45070112068628987
1105	10	0.3577772085151949
1105	11	0.5500704576345044
1105	12	0.3443053503987983
1105	13	0.5969262321198556
1105	14	0.5322552805798111
1105	15	0.6069779462722806
1105	16	0.6335944891180745
1105	17	0.6099916913450585
1105	18	0.5518700763839419
1105	19	0.490052092433082
1105	20	0.504494202573909
1105	21	0.5767268778619155
1105	22	0.5481288593052582
1105	23	0.5879882183720042
1105	24	0.6619376071437983
1105	25	0.590007801916073
1105	26	0.46462321903924675
1105	27	0.5873833886888846
1105	28	0.6407911496968239
1105	29	0.6135004517094771
1105	30	0.5775935193407165
1105	31	0.5823676000610547
1105	32	0.5740259464531619
1105	33	0.6439001954104384
1105	34	0.6066026180183499
1105	35	0.7170266088670516
1105	36	0.6680852410216289
1105	37	0.6912812943171431
1105	38	0.6030130941776335
1105	39	0.47350917522265945
1105	40	0.6768475255265543
1105	41	0.6583181282527732
1105	42	0.48403341758626084
1105	43	0.7824680152452999
1105	44	0.8006825521119154
1105	45	0.8339002480163131
1105	46	0.7372254705372541
1105	47	0.7749102933527752
1105	48	0.7304140952329234
1105	49	0.6671706553917645
1105	50	0.8194972066133417
1106	1	0.09107102135928713
1106	2	0.2740685173943618
1106	3	0.09424818757998565
1106	4	0.10549134683767392
1106	5	0.15820898904753022
1106	6	0.10227350955809555
1106	7	0.13707183655653665
1106	8	0.1482081659305834
1106	9	0.3115736237480746
1106	10	0.16576481194179138
1106	11	0.1653618388323821
1106	12	0.11814032913685679
1106	13	0.18716620457191624
1106	14	0.192544339044467
1106	15	0.18004918433781825
1106	16	0.23514795908658925
1106	17	0.27080377085724905
1106	18	0.29871911821587815
1106	19	0.17534090725439455
1106	20	0.18463726994617247
1106	21	0.2329181801193556
1106	22	0.2995080430675685
1106	23	0.015177841114649482
1106	24	0.09479015094275667
1106	25	0.21947792636908758
1106	26	0.32214968682797007
1106	27	0.2004153839329632
1106	28	0.23730867415049772
1106	29	0.16220962926699656
1106	30	0.2506465653698129
1106	31	0.27543903304126616
1106	32	0.14809222394019628
1106	33	0.34164795632809586
1106	34	0.13857479719691668
1106	35	0.17661982193803283
1106	36	0.4195345940673363
1106	37	0.30258699524656407
1106	38	0.2571873543952208
1106	39	0.41896165340033775
1106	40	0.24785624146115026
1106	41	0.2299369271235676
1106	42	0.26039578995775514
1106	43	0.35523193783818385
1106	44	0.2885900456066395
1106	45	0.2113366002531194
1106	46	0.2533017081642299
1106	47	0.3970763151767187
1106	48	0.295743972079336
1106	49	0.25028227328103014
1106	50	0.40941719413880406
1107	1	0.33695864921412877
1107	2	0.5311186318215277
1107	3	0.42597487012633906
1107	4	0.525000010608629
1107	5	0.4047084519404365
1107	6	0.40876549965663855
1107	7	0.5275346827570857
1107	8	0.41859721828684826
1107	9	0.49123361160478396
1107	10	0.5711031894113177
1107	11	0.45966850363085143
1107	12	0.5345291012633382
1107	13	0.7932144975054527
1107	14	0.6707004270366212
1107	15	0.6836127731473098
1107	16	0.7260545024521505
1107	17	0.6155191040971744
1107	18	0.6741242837849768
1107	19	0.6697363114858632
1107	20	0.6621348179355238
1107	21	0.7416426675323677
1107	22	0.6114925370192736
1107	23	0.6117870606746862
1107	24	0.5181519743045797
1107	25	0.5822073645083634
1107	26	0.4886015106568422
1107	27	0.4814858617247566
1107	28	0.517024707964886
1107	29	0.45384477008246904
1107	30	0.6294051474070841
1107	31	0.7577051579303458
1107	32	0.553239111054296
1107	33	0.5518763436056631
1107	34	0.4707825408800659
1107	35	0.5107658216578059
1107	36	0.5540495630085331
1107	37	0.5153000866481747
1107	38	0.4520638197634952
1107	39	0.37266302185580713
1107	40	0.44960300714234336
1107	41	0.45422817928366754
1107	42	0.4240406454058885
1107	43	0.4301467012775362
1107	44	0.5553828156780938
1107	45	0.7002721941067449
1107	46	0.7056673146282009
1107	47	0.715076408586647
1107	48	0.7100310207926128
1107	49	0.6608469363047078
1107	50	0.7214250405728819
1108	1	0.22416526436542483
1108	2	0.39021025214843164
1108	3	0.1513160992734477
1108	4	0.3035585431427563
1108	5	0.3805858983187136
1108	6	0.3769409167144886
1108	7	0.3707088961847873
1108	8	0.3801063297670737
1108	9	0.3948294566735058
1108	10	0.3732679182334451
1108	11	0.3264224334832875
1108	12	0.2192965599186394
1108	13	0.322703436144387
1108	14	0.4276586129252635
1108	15	0.4020668305845376
1108	16	0.3648902310094826
1108	17	0.28122647753546204
1108	18	0.19429531962908442
1108	19	0.366305363747486
1108	20	0.28278008405814603
1108	21	0.37968700926703747
1108	22	0.4350060537858411
1108	23	0.3437717264265956
1108	24	0.36165417694775215
1108	25	0.36679427864139313
1108	26	0.322252602349387
1108	27	0.23221345614953748
1108	28	0.20305537646107297
1108	29	0.23412028102346483
1108	30	0.3176276264037412
1108	31	0.3219615552245827
1108	32	0.26078346482526626
1108	33	0.12624263233246324
1108	34	0.16491364027584865
1108	35	0.20344594490953227
1108	36	0.09165578336914137
1108	37	0.17045162368387423
1108	38	0.3275181901445592
1108	39	0.28115463255367246
1108	40	0.31696777929515463
1108	41	0.19402380288445187
1108	42	0.3463625683254179
1108	43	0.20506846531788625
1108	44	0.2809768071563923
1108	45	0.0816147231744182
1108	46	0.23516347504802582
1108	47	0.0672477573745951
1108	48	0.023114480507111068
1108	49	0.18444771625704703
1108	50	0.06519866181454859
1109	1	0.3950109838358351
1109	2	0.44874713597542026
1109	3	0.4182979408980323
1109	4	0.40773580517969066
1109	5	0.38667122238781104
1109	6	0.5121685828678011
1109	7	0.4645820860180052
1109	8	0.3595763821768472
1109	9	0.4380415550940526
1109	10	0.43238978588384214
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2211	7	0.05410283071661581
2211	8	0.031164353546657253
2211	9	-0.020680537215248762
2211	10	-0.16763169692337954
2211	11	-0.102039739335126
2211	12	-0.12163797662966402
2211	13	-0.025226881522293354
2211	14	-0.09644068652330873
2211	15	-0.19290283735549707
2211	16	-0.07391681414529216
2211	17	-0.24434813272529254
2211	18	-0.10567264943244936
2211	19	-0.015008801359911672
2211	20	-0.04206583709973444
2211	21	-0.15676101330917191
2211	22	-0.09152683013120821
2211	23	-0.05469521268984323
2211	24	-0.1328389201785498
2211	25	-0.18697788051520803
2211	26	-0.21274075550756777
2211	27	-0.138679741021825
2211	28	-0.1876111738009527
2211	29	-0.05856138689770063
2211	30	-0.14146656461155516
2211	31	-0.27651765356994673
2211	32	-0.14961957050432773
2211	33	0.004825319344774925
2211	34	0.067803857331171
2211	35	-0.05896265188761163
2211	36	-0.15533283650443508
2211	37	-0.18119322104571012
2211	38	-0.2434955212207015
2211	39	-0.228468216418392
2211	40	-0.238742458099523
2211	41	-0.27495337787386376
2211	42	-0.31923177960018023
2211	43	-0.306617518226588
2211	44	-0.2767815226572736
2211	45	-0.3855479609592365
2211	46	-0.3711521057914595
2211	47	-0.08162178055405178
2211	48	-0.25009231783837294
2211	49	-0.041570372079917206
2211	50	-0.05249940500328876
2212	1	-0.9921493238013532
2212	2	-2.465038754548729
2212	3	-1.2373705806059476
2212	4	-0.7295562479114182
2212	5	-0.558469219421724
2212	6	-0.41675618932235636
2212	7	-0.47320401252527944
2212	8	-0.5043629224613966
2212	9	-0.7111263683549041
2212	10	-0.4347463684201358
2212	11	-0.6434062863536525
2212	12	-0.5177376679351724
2212	13	-1.943969022831972
2212	14	-1.4232402909192672
2212	15	-0.9371143873033674
2212	16	-0.8095753110098087
2212	17	-0.659478274350171
2212	18	-0.7957587367097361
2212	19	-0.5215746978701645
2212	20	-0.7708617915230391
2212	21	-0.7574310505283819
2212	22	-0.6166914843259903
2212	23	-0.6766332499501715
2212	24	-0.6258778764416917
2212	25	-0.6893907339921057
2212	26	-0.8285580079095709
2212	27	-1.1086291370518566
2212	28	-0.9272434127896503
2212	29	-0.9098886704432445
2212	30	-0.8577008914851021
2212	31	-0.7846235964243462
2212	32	-0.8335803047472581
2212	33	-0.8093124431397669
2212	34	-0.8349947786114669
2212	35	-0.9324921274724218
2212	36	-0.8261466456437532
2212	37	-0.6484502483279968
2212	38	-0.768388368941693
2212	39	-0.7488628383047526
2212	40	-0.5829199751359582
2212	41	-0.6101781264243229
2212	42	-0.5911544563686064
2212	43	-0.7721783246467027
2212	44	-0.9456535714728034
2212	45	-0.8884359047432682
2212	46	-0.7781885815350122
2212	47	-0.8658322798214257
2212	48	-0.8048844486983384
2212	49	-0.588677688813263
2212	50	-0.6561059228768471
2213	1	-0.4991493587184621
2213	2	-1.5145743007254744
2213	3	-0.8743360250612788
2213	4	-0.38460396607855785
2213	5	-0.45444130601163835
2213	6	-0.32019884698716794
2213	7	-0.3300562692403517
2213	8	-0.361620668145477
2213	9	-0.545314326008338
2213	10	-0.2800802041092467
2213	11	-0.40383551895451575
2213	12	-0.38618916188043073
2213	13	-0.45796543200187756
2213	14	-0.46076407115776163
2213	15	-0.5452337970465919
2213	16	-0.5898808244207956
2213	17	-0.42444954658306955
2213	18	-0.6018068862720938
2213	19	-0.6294513774111081
2213	20	-0.6896417878511159
2213	21	-0.6929048024389672
2213	22	-0.6783925443417402
2213	23	-0.6155128276108854
2213	24	-0.5767238841212006
2213	25	-0.5916670476918924
2213	26	-0.46179295385000785
2213	27	-0.5055431407260329
2213	28	-0.47729257832714467
2213	29	-0.5064888143918225
2213	30	-0.6702008210877111
2213	31	-0.48772710762091265
2213	32	-0.5957584901165447
2213	33	-0.5583831975857834
2213	34	-0.7082662410862781
2213	35	-0.761860679775431
2213	36	-0.6624417379882388
2213	37	-0.6468226606607175
2213	38	-0.9355289508408902
2213	39	-0.8780994940570039
2213	40	-0.6545200346076783
2213	41	-0.7941129913796061
2213	42	-0.8674093039329165
2213	43	-0.9212324089906616
2213	44	-0.8028074106085725
2213	45	-1.0415826117786082
2213	46	-0.9784545147171926
2213	47	-0.9613336612998182
2213	48	-1.1864391497234408
2213	49	-0.9428855379533445
2213	50	-1.1546013898480822
2214	1	-0.9827288029078254
2214	2	-1.796525513012753
2214	3	-1.05463218092655
2214	4	-0.6058700505050325
2214	5	-0.5901947705711876
2214	6	-0.820941869732053
2214	7	-0.6238382467834569
2214	8	-0.7470923106783047
2214	9	-0.8021681554142646
2214	10	-0.9656194839284851
2214	11	-0.6940332140206138
2214	12	-0.7689725925554995
2214	13	-0.8029501518995801
2214	14	-0.7570203226697245
2214	15	-0.9302578156888046
2214	16	-0.9365350826899075
2214	17	-0.8553591282028421
2214	18	-0.9367842499977712
2214	19	-0.9916292980335655
2214	20	-1.073394521730485
2214	21	-0.9388979926313965
2214	22	-0.8037297022262564
2214	23	-0.8100078765944332
2214	24	-0.773229126422315
2214	25	-0.8493214985050983
2214	26	-0.9050862478845971
2214	27	-0.8005511469401085
2214	28	-0.934608678140691
2214	29	-0.8141463097057624
2214	30	-0.9053802738345951
2214	31	-1.0975167408490876
2214	32	-1.0715366069035923
2214	33	-1.1218635062487863
2214	34	-1.0921917709528302
2214	35	-1.0670817516700808
2214	36	-1.0388193745670122
2214	37	-1.1671097323940884
2214	38	-1.2583357172026126
2214	39	-1.2366231909695529
2214	40	-1.413136383082879
2214	41	-1.3017801961823423
2214	42	-1.3037413881981124
2214	43	-1.1684573877203022
2214	44	-1.088852242116026
2214	45	-1.1012309637163114
2214	46	-0.9331934858075996
2214	47	-1.0558086330353333
2214	48	-1.0720383321515712
2214	49	-1.0010157537179296
2214	50	-1.124299556400377
2215	1	-0.480269383330846
2215	2	-0.27340696898914274
2215	3	-0.6963494023289662
2215	4	-0.4388481343439139
2215	5	-0.533464158995514
2215	6	-0.729943345383802
2215	7	-0.7540508499424448
2215	8	-0.7141907421151501
2215	9	-0.8184003246223313
2215	10	-1.1443919602907258
2215	11	-1.0365757073255253
2215	12	-0.9181500533414209
2215	13	-0.8600086860855143
2215	14	-1.0667432280640554
2215	15	-1.080938255105103
2215	16	-1.061118347706028
2215	17	-1.1257039934813093
2215	18	-1.1550167471284496
2215	19	-0.9417030806208757
2215	20	-1.1306989978741129
2215	21	-1.1041436613202507
2215	22	-1.1173603520595563
2215	23	-0.9888623336092479
2215	24	-1.0492853104962094
2215	25	-1.1621323418127432
2215	26	-1.1371734752989298
2215	27	-1.2969986519955796
2215	28	-1.2923995728228803
2215	29	-1.3089268748257297
2215	30	-1.2133269317566615
2215	31	-1.0216846372255404
2215	32	-0.8860417170309347
2215	33	-1.0778398958208513
2215	34	-1.0881747189770934
2215	35	-1.144498814576572
2215	36	-1.18660316425931
2215	37	-1.0687876030832726
2215	38	-1.1701518619257953
2215	39	-1.2106626509903222
2215	40	-0.9834757829844989
2215	41	-1.0813342214323853
2215	42	-1.0541103011346564
2215	43	-0.9652000231250142
2215	44	-0.9816762023280816
2215	45	-0.9433635537583953
2215	46	-0.6960507848183681
2215	47	-0.6709914498905505
2215	48	-0.7059127377918415
2215	49	-0.8027318820449807
2215	50	-0.8138661520171411
2216	1	-1.0514001735183243
2216	2	-0.6469859876173629
2216	3	-0.8901882514019093
2216	4	-2.051211566014974
2216	5	-1.3538480962715909
2216	6	-1.142206391606301
2216	7	-1.26638824070033
2216	8	-1.0835843236159164
2216	9	-1.4137293165281335
2216	10	-1.366321996080842
2216	11	-1.3651355109163035
2216	12	-1.2534790595948309
2216	13	-1.3238521096549576
2216	14	-1.4917548436504404
2216	15	-1.6238305564403177
2216	16	-1.6833326096613561
2216	17	-1.3663158138559197
2216	18	-1.4422083243158426
2216	19	-1.3079028840107867
2216	20	-1.4263379699060792
2216	21	-1.329171107103619
2216	22	-1.4517974151897661
2216	23	-1.4064261045534678
2216	24	-1.3765490465996493
2216	25	-1.373929616771072
2216	26	-1.5725366527781546
2216	27	-1.6843915500718296
2216	28	-1.704789117145161
2216	29	-1.5705852051321116
2216	30	-1.6071157554941138
2216	31	-1.6212636305063128
2216	32	-1.500668245141128
2216	33	-1.51417560216926
2216	34	-1.5683191802853003
2216	35	-1.5675699158345964
2216	36	-1.8713094005199764
2216	37	-1.820640895895771
2216	38	-1.9142569667817142
2216	39	-1.8431961927147285
2216	40	-1.911169086548135
2216	41	-1.8677535384813382
2216	42	-1.825477061690492
2216	43	-1.6459192975376524
2216	44	-1.6708156111252208
2216	45	-1.5047279254370736
2216	46	-1.6812088990736491
2216	47	-1.8861910215665532
2216	48	-1.7952825692145886
2216	49	-1.820262749135651
2216	50	-1.9652367912103692
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28898.0
3	4574.0
4	8066.0
5	4427.0
6	3183.0
7	2329.0
8	1998.0
9	1799.0
10	1819.0
11	1858.0
12	2000.0
13	2284.0
14	2567.0
15	2604.0
16	2873.0
17	2961.0
18	3223.0
19	3586.0
20	4703.0
21	6139.0
22	8068.0
23	9887.0
24	11495.0
25	12741.0
26	12861.0
27	13931.0
28	16324.0
29	19117.0
30	21372.0
31	25004.0
32	30672.0
33	38427.0
34	48226.0
35	61751.0
36	79503.0
37	99136.0
38	153710.0
39	420246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.440976051795374	24.238540095506657	16.54818470070919	22.772299151988783
2	12.164485465613833	25.065120964480016	41.449972878682985	21.320420691223166
3	13.0447037264743	33.50241920238701	32.39236209865168	21.060514972487002
4	9.462534253691054	20.39978677130541	44.10802451789367	26.029654457109864
5	8.7796670812533	42.32549026289336	37.966196991123304	10.928645664730036
6	22.132152211409466	47.27471731862609	19.189455079747024	11.403675390217414
7	22.260666615558904	35.19195223127966	27.928261739878536	14.619119413282908
8	22.33342957865841	37.80601541799004	23.271182701734034	16.589372301617516
9	25.0320701198477	17.07280300513633	24.31835024148417	33.5767766335318
10	13.334701894918322	29.60326478600183	37.773066367286724	19.288966951793128
11	25.890112923120956	30.180101133320157	28.79582177637542	15.133964167183464
12	18.93021888856234	32.21627331467733	32.042746947103	16.810760849657335
13	24.283002988268407	25.769385411662082	31.313100336989447	18.634511263080064
14	19.63115134653015	25.398149197227287	33.07694470166603	21.893754754576534
15	19.91737050808619	34.49585361802175	26.12744534314665	19.45933053074541
16	19.264003434889172	30.880692975259766	30.580906889116633	19.274396700734428
17	14.983374807572874	33.44615575969419	31.776310016603894	19.794159416129034
18	15.62503993477533	31.193340540761582	34.59591119017132	18.585708334291766
19	18.180788082071224	29.7174637766973	35.40925282063384	16.692495320597633
20	16.110814830969815	31.315353199058794	35.73538908208626	16.838442887885133
21	18.090846977077828	29.832305047376124	34.50329613783632	17.573551837709726
22	17.64576090262331	28.695638098759353	35.02947207543215	18.62912892318518
23	18.072859828784598	30.57708739584425	34.087607968016165	17.26244480735499
24	17.801119374377063	31.071754112465605	33.603976590252756	17.52314992290458
25	18.096220587007597	29.736959125502814	35.232893142945734	16.933927144543848
26	16.720179715246406	29.996072775474353	36.58818748791378	16.695560021365463
27	16.45136447973939	31.79782153487572	33.74371940633162	18.007094579053273
28	17.90812223562525	27.138322667702564	36.793785310734464	18.159769785937723
29	16.770396393510772	29.641770396393515	36.266360219578765	17.321472990516952
30	17.068398303799754	30.522234091307908	33.84725338567089	18.562114219221446
31	18.759817367156753	28.229381399390086	36.030120789818895	16.98068044363426
32	16.76570299323668	30.517811206323785	35.59140700863728	17.125078791802245
33	17.56919046264388	30.624370782861103	34.43961985489891	17.366818899596108
34	18.284355935076096	27.425384098464118	36.650135038109624	17.64012492835017
35	16.738111157008955	31.71198487358456	34.89653821421423	16.653365755192254
36	17.370518539086117	29.870358840528855	33.55483878111247	19.20428383927256
37	19.582538072449438	27.50746714837971	34.66375049717113	18.246244281999722
38	16.73075694666241	30.33722985201746	36.26402640264026	16.66798679867987
39	17.127836428238066	31.15054829379963	33.03712863698147	18.68448664098083
40	17.00274980988075	28.875303062431502	36.99103182166176	17.130915306025994
41	17.0592543389028	29.675880980362102	35.351966344761806	17.912898335973292
42	17.754474470587333	29.838461754634572	33.57703021358747	18.830033561190625
43	17.121792207969154	29.034903366010177	35.12349190345457	18.719812522566098
44	16.415724337843514	30.671962272216717	34.5594465501781	18.352866839761663
45	17.74599960317494	29.662162219699724	33.73217374125132	18.859664435874006
46	18.106728158944367	28.38281327170481	33.82975063609765	19.680707933253174
47	17.832852927777378	30.60437431611395	33.86582421840074	17.696948537707932
48	17.186543352955553	30.925304147233913	32.124010834939185	19.764141664871346
49	18.265422642611597	28.97175570374016	34.29533167399122	18.467489979657024
50	17.33605482494235	29.37848488873902	33.64686687852746	19.638593407791163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5844.0
1	3636.5
2	1429.0
3	2392.0
4	3355.0
5	3285.0
6	3215.0
7	2402.0
8	1589.0
9	2067.5
10	2546.0
11	3561.0
12	4576.0
13	7997.5
14	11419.0
15	14373.5
16	17328.0
17	18144.5
18	18961.0
19	17209.5
20	15458.0
21	15539.0
22	15620.0
23	18491.5
24	21363.0
25	28605.0
26	35847.0
27	44275.5
28	52704.0
29	59375.5
30	66047.0
31	72595.0
32	79143.0
33	95459.5
34	111776.0
35	109390.5
36	107005.0
37	111883.0
38	116761.0
39	120358.0
40	123955.0
41	117211.0
42	110467.0
43	89161.5
44	67856.0
45	59873.0
46	51890.0
47	45992.5
48	40095.0
49	34031.0
50	27967.0
51	24560.5
52	21154.0
53	16071.0
54	10988.0
55	8929.0
56	6870.0
57	5518.0
58	4166.0
59	3740.0
60	3314.0
61	3106.0
62	2898.0
63	2563.5
64	2229.0
65	1944.0
66	1659.0
67	1442.0
68	1225.0
69	1235.5
70	1246.0
71	1166.5
72	1087.0
73	1028.0
74	969.0
75	850.5
76	732.0
77	658.0
78	584.0
79	492.5
80	401.0
81	349.5
82	298.0
83	229.0
84	160.0
85	122.0
86	84.0
87	64.5
88	45.0
89	33.0
90	21.0
91	14.5
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01728598166493807
2	7.66373571351934E-4
3	6.812209523128303E-4
4	0.003065494285407736
5	0.0427466147576301
6	0.028696432616177978
7	0.03167677428254661
8	0.02375758071190996
9	0.03167677428254661
10	0.03235799523485944
11	0.039681120472222366
12	0.0406177992816525
13	0.03729684713912746
14	0.04198024118627817
15	0.03806322071047939
16	0.044705124995529484
17	0.04606756690015515
18	0.04879245070940647
19	0.05117672404250138
20	0.046663635233428875
21	0.04802607713805453
22	0.045897261662076944
23	0.04419420928129487
24	0.041895088567239065
25	0.0405326466626134
26	0.04300207261474741
27	0.04325753047186472
28	0.04240600428147369
29	0.04070295190069161
30	0.037807762853362084
31	0.035934405234501796
32	0.032528300472937643
33	0.024183343807105476
34	0.021203002140736844
35	0.021884223093049673
36	0.02341697023575354
37	0.01864842356956373
38	0.020181170712267597
39	0.014816555712804057
40	0.008430109284871274
41	0.008685567141988586
42	0.007578583094480237
43	0.0028951890473295286
44	0.004683394047150708
45	0.003491257380603255
46	0.001192136666547453
47	5.960683332737265E-4
48	0.0011069840475083491
49	8.515261903910379E-5
50	5.109157142346227E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1174362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.60373099558911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54765329820486	15.986382585795733
2	7.118132683970948	2.7908391665495436
3	2.691409229612254	1.5828498760909304
4	1.3789402670348605	1.0812949621574888
5	0.9158934456608179	0.8977464364678942
6	0.6332275412840466	0.7448173426998449
7	0.4655899235175298	0.6389109730426193
8	0.386325750645284	0.6058740873855348
9	0.34644566547123795	0.6112464867437404
>10	2.975527262252955	12.845278175491192
>50	0.6856648717158891	9.51430783856755
>100	0.7435081120247341	30.508483426396705
>500	0.0814254844407698	10.755626871583242
>1k	0.028031724151740427	8.351492573979884
>5k	0.0022247400120428908	3.0848491970481238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9214	0.7845962318263023	No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA	8725	0.7429566011161806	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	6491	0.5527256501828227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5869	0.49976072114050013	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5067	0.4314683206711389	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3124	0.2660167818781602	No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	3031	0.2580975883075236	No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC	2564	0.21833131521626212	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	2493	0.21228547926448574	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2456	0.20913483236003888	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	2403	0.20462174355096638	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2233	0.19014579831431877	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	2219	0.1889536616477713	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	2154	0.18341874141022957	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2044	0.17405195331592813	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	1996	0.16996462760205117	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	1945	0.16562184403105687	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1860	0.15838387141273302	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	1748	0.1488467780803534	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	1718	0.14629219950918032	No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA	1716	0.14612189427110212	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	1691	0.14399307879512452	No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC	1647	0.14024636355740394	No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT	1625	0.13837300593854365	No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	1619	0.13786209022430904	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1617	0.13769178498623083	No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG	1605	0.13666995355776157	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	1576	0.13420052760562756	No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG	1543	0.13139049117733714	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	1522	0.12960228617751596	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	1501	0.12781408117769477	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1499	0.12764377593961657	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	1469	0.12508919736844346	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	1405	0.11963942974994082	No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGT	1372	0.1168293933216504	No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG	1366	0.11631847760741577	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	1327	0.11299752546489072	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	1321	0.1124866097506561	No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA	1296	0.11035779427467851	No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC	1294	0.1101874890366003	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1266	0.10780321570350539	No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	1253	0.10669623165599704	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	1249	0.10635562117984063	No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG	1238	0.10541894237041048	No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC	1219	0.1038010426086675	No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC	1218	0.10371588998962841	No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG	1215	0.1034604321325111	No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA	1201	0.10226829546596365	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1186	0.10099100618037708	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	1185	0.10090585356133798	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	1185	0.10090585356133798	No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT	1178	0.10030978522806426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.515261903910379E-5	0.0
11	0.0	0.0	0.0	8.515261903910379E-5	0.0
12	0.0	0.0	0.0	8.515261903910379E-5	0.0
13	0.0	0.0	0.0	8.515261903910379E-5	0.0
14	0.0	0.0	0.0	1.7030523807820758E-4	0.0
15	0.0	0.0	0.0	2.554578571173114E-4	0.0
16	0.0	0.0	0.0	3.4061047615641517E-4	0.0
17	0.0	0.0	0.0	4.257630951955189E-4	0.0
18	0.0	0.0	0.0	5.109157142346227E-4	0.0
19	0.0	0.0	0.0	5.960683332737265E-4	0.0
20	0.0	0.0	0.0	7.663735713519341E-4	0.0
21	0.0	0.0	0.0	0.001192136666547453	0.0
22	0.0	0.0	0.0	0.0014475945236647643	0.0
23	0.0	0.0	0.0	0.0024694259521340096	0.0
24	0.0	0.0	0.0	0.0040021730948378775	0.0
25	0.0	0.0	0.0	0.004342783570994293	0.0
26	0.0	0.0	0.0	0.00630129380889368	0.0
27	0.0	0.0	0.0	0.007152819999284718	0.0
28	0.0	0.0	0.0	0.009537093332379624	0.0
29	0.0	0.0	0.0	0.013794724284334814	0.0
30	0.0	0.0	0.0	0.02069208642650222	0.0
31	0.0	0.0	0.0	0.03414620023468062	0.0
32	0.0	0.0	0.0	0.04572695642399873	0.0
33	0.0	0.0	0.0	0.05875530713698161	0.0
34	0.0	0.0	0.0	0.07399762594498119	0.0
35	0.0	0.0	0.0	0.0920499811812712	0.0
36	0.0	0.0	0.0	0.11435996736951638	0.0
37	0.0	0.0	0.0	0.14339701046185077	0.0
38	0.0	0.0	0.0	0.1732855797445762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	20	7.8523945E-4	44.004726	2
GACAGGC	25	0.0023520172	35.202282	20
ATTGCCG	40	1.819221E-5	33.00214	8
TGCCGCT	50	2.3601824E-6	30.801996	10
GGTATCA	2010	0.0	28.351303	1
ATGTATT	70	3.5868652E-8	28.288754	1
TTGGACG	55	4.9567425E-6	28.00301	17
TGGACGT	55	4.9567425E-6	28.00301	18
GTACAAC	40	7.027596E-4	27.502954	1
TGCACAG	40	7.029346E-4	27.501783	12
GCGAATA	65	5.9386366E-7	27.072912	33
AATATCG	65	5.9386366E-7	27.072912	36
TTGCCGC	50	8.3375504E-5	26.40171	9
ATATTGC	50	8.339967E-5	26.400585	6
CAACAGT	85	8.229108E-9	25.884033	23
GTATCAA	4785	0.0	24.50838	1
ACAGTAA	90	1.5113983E-8	24.44603	25
AATATTG	265	0.0	24.069859	41
CGCTACC	55	1.5924186E-4	24.002579	13
CCGCTAC	55	1.5928787E-4	24.001558	12
>>END_MODULE