FastQCFastQC Report
Mon 6 Feb 2017
SRR2077469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894176
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.10089425850295416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53000.025.363871
GGTATCA22300.025.1585451
TATCAAC70800.018.7069192
CAACGCA70850.018.506455
TCAACGC71150.018.4293654
ATCAACG71500.018.3689793
AACGCAG73150.017.9236436
GCGATAC2950.016.40697714
ACGCAGA86100.015.152337
TGCGATA3250.014.89325213
GTACATG100900.014.5658391
CGCAGAG90050.014.3655248
TACATGG101100.014.1884672
AGAGTAC87350.014.0293311
GAGTACT43700.013.69423212
GTCCTAT7100.013.6346671
TATACTG8150.013.4967475
GTATTAT9400.013.341271
CAGAGTA94400.013.2847410
GTCTTAC5150.013.2435581
Produced by FastQC (version 0.11.5)