FastQCFastQC Report
Mon 6 Feb 2017
SRR2077469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894176
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.12808871504523678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.030.112471
GTATCAA63800.024.9370021
TATCAAC90100.017.4365672
ATCAACG92500.016.9603733
TCAACGC93150.016.794784
CAACGCA94000.016.5965255
AACGCAG97300.016.1220266
GTACATG100750.014.54641151
GTATTAC4250.013.9798161
TACATGG102600.013.961152
CGTGCCG4150.013.78430710
TGCGATA3051.8189894E-1213.706614513
CGTACAC3400.013.5902663
ACATGGG103450.013.5273973
GCGATAC2959.094947E-1213.42539114
ACGCAGA116350.013.4069017
CTAGCGT1000.00876088513.20095523
GTGGTAT11700.013.16554451
GAGTACT75550.013.07606812
CGCAGAG119050.013.0473968
Produced by FastQC (version 0.11.5)