FastQCFastQC Report
Mon 6 Feb 2017
SRR2077468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888580
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41800.025.4218331
GGTATCA18050.024.2555921
TATCAAC54700.019.104462
TCAACGC53550.018.9395644
ATCAACG54700.018.8624883
CAACGCA55250.018.4361275
AACGCAG56400.017.980646
ACGCAGA66650.015.1502037
ATAACGC1603.829664E-715.1246793
GTACATG68550.014.6992111
CGCAGAG68450.014.6875278
TAACGCA1351.9575878E-514.6668634
AGAGTAC66200.014.48987111
TACATGG67700.014.4285542
GAGTACT38650.013.946094512
CAGAGTA71650.013.78688610
GTATTAG5050.013.5053641
ACATGGG72250.013.4889853
GCAGAGT74700.013.2828719
AGTACTT41700.013.18985313
Produced by FastQC (version 0.11.5)