FastQCFastQC Report
Mon 6 Feb 2017
SRR2077468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888580
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.10932707420254935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.027.168471
GTATCAA47850.025.3381881
TATCAAC66600.018.0709692
ATCAACG68000.017.7636323
TCAACGC68600.017.5120454
CAACGCA69700.017.1728345
AACGCAG72600.016.5157416
ACGCAGA84300.014.2232757
TTACGTA1251.6707985E-414.0814794
CGCAGAG86200.013.7821578
TCGAACT5450.013.72668719
CGAACTC5300.013.69931520
GAGTACT56300.013.63908912
GCGATAC1150.001423203313.39317614
GTACATG65800.013.2092191
AGTACTT58550.013.1154113
AGAGTAC86100.013.05783111
CGCGATC1353.2087E-413.03750423
TGCGATA1200.001960726712.83512713
TAAACCG1200.00196098212.8349045
Produced by FastQC (version 0.11.5)