FastQCFastQC Report
Mon 6 Feb 2017
SRR2077467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345944
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33590.10039020378105551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.027.0882281
GTATCAA44500.023.9305631
TCAACGC60400.017.5211544
TATCAAC59850.017.4975762
CAACGCA60800.017.4410275
ATCAACG60800.017.4402453
CGTGCCG2850.016.98395710
GCACCGT4300.016.8821836
AACGCAG63650.016.658346
CGCACGA3000.015.40113135
GTACATG76750.015.3084221
ACGCAGA71650.014.6768827
GGACCGA3050.014.4249156
TACATGG79500.014.3903482
CGCAGAG75100.013.8565888
GTGCCGA3350.013.79164511
ACATGGG80900.013.7053813
AGAGTAC71400.013.43475811
GAGTACT39600.013.3896712
GTATTAT6850.012.8480431
Produced by FastQC (version 0.11.5)