##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077467_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3345944 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.706415588545415 31.0 30.0 34.0 16.0 34.0 2 28.68681693417463 31.0 30.0 34.0 16.0 34.0 3 28.772257993558767 31.0 30.0 34.0 16.0 34.0 4 32.111680291122624 35.0 33.0 37.0 19.0 37.0 5 32.16788714933663 35.0 33.0 37.0 19.0 37.0 6 32.24533255786708 36.0 33.0 37.0 19.0 37.0 7 32.03068820040025 36.0 33.0 37.0 17.0 37.0 8 31.96292018037361 36.0 33.0 37.0 17.0 37.0 9 33.162838349954455 38.0 33.0 39.0 17.0 39.0 10 33.03969731710991 38.0 33.0 39.0 16.0 39.0 11 33.114457085952424 38.0 33.0 39.0 16.0 39.0 12 33.03120733640491 38.0 33.0 39.0 15.0 39.0 13 33.036944730694835 38.0 33.0 39.0 15.0 39.0 14 33.85006055092374 39.0 33.0 41.0 11.0 41.0 15 33.95173170860002 39.0 33.0 41.0 13.0 41.0 16 33.84330610434604 39.0 33.0 41.0 11.0 41.0 17 33.65881646554754 39.0 33.0 41.0 10.0 41.0 18 33.46643548128719 38.0 32.0 41.0 10.0 41.0 19 33.812344139650875 39.0 33.0 41.0 11.0 41.0 20 33.85921461925244 39.0 33.0 41.0 11.0 41.0 21 33.853626360752 39.0 33.0 41.0 10.0 41.0 22 33.75725296059946 39.0 33.0 41.0 10.0 41.0 23 33.49042721575735 39.0 33.0 41.0 10.0 41.0 24 33.63746285054382 39.0 33.0 41.0 10.0 41.0 25 33.54001083102407 39.0 33.0 41.0 10.0 41.0 26 33.58632362047901 39.0 33.0 41.0 10.0 41.0 27 33.57097937084422 39.0 33.0 41.0 9.0 41.0 28 33.444537326386815 39.0 33.0 41.0 8.0 41.0 29 33.40843480942897 39.0 33.0 41.0 8.0 41.0 30 33.17780990955019 38.0 32.0 41.0 8.0 41.0 31 33.108789328213504 38.0 32.0 41.0 7.0 41.0 32 33.12254090325481 38.0 32.0 41.0 2.0 41.0 33 33.11173348986116 38.0 32.0 41.0 2.0 41.0 34 33.09458018424696 38.0 32.0 41.0 2.0 41.0 35 33.02317492462516 38.0 32.0 40.0 2.0 41.0 36 32.894034090229844 38.0 32.0 40.0 2.0 41.0 37 32.80017806633943 38.0 32.0 40.0 2.0 41.0 38 32.69067444045687 38.0 32.0 40.0 2.0 41.0 39 32.62677558261585 38.0 32.0 40.0 2.0 41.0 40 32.58474947578322 38.0 32.0 40.0 2.0 41.0 41 32.470883553341004 38.0 32.0 40.0 2.0 41.0 42 32.28160363712005 38.0 31.0 40.0 2.0 41.0 43 32.16700339276449 38.0 31.0 40.0 2.0 41.0 44 32.096125039749616 38.0 31.0 40.0 2.0 41.0 45 32.003188039010816 38.0 31.0 40.0 2.0 41.0 46 31.840639592294433 38.0 31.0 40.0 2.0 41.0 47 31.65164479740247 38.0 31.0 40.0 2.0 41.0 48 31.46040190750353 38.0 30.0 40.0 2.0 41.0 49 31.323507506401782 38.0 30.0 40.0 2.0 41.0 50 31.11837526270613 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9361804157970326 1101 2 0.9040924750540142 1101 3 1.0128584905939881 1101 4 0.9844456756078017 1101 5 0.836462613056689 1101 6 0.959195260713912 1101 7 1.041010468024929 1101 8 0.955687760434067 1101 9 1.1733829277364833 1101 10 1.1734775689284476 1101 11 1.1789594617365466 1101 12 1.2168668367927609 1101 13 1.104272617945064 1101 14 1.5085922870066426 1101 15 1.390169314459449 1101 16 1.2381262550550645 1101 17 1.4750925308964398 1101 18 1.5914745084483144 1101 19 1.235470808879569 1101 20 1.1789938789631051 1101 21 1.2962106903054007 1101 22 1.2103681997941393 1101 23 1.3776856752337423 1101 24 1.315918024121558 1101 25 1.351547066559938 1101 26 1.2953755307966972 1101 27 1.3559897667227645 1101 28 1.5140503754326389 1101 29 1.4756808940626414 1101 30 1.5936541279263352 1101 31 1.5893198902221854 1101 32 1.5156408017269953 1101 33 1.5060314064769074 1101 34 1.4904645274919162 1101 35 1.4099012088195764 1101 36 1.5047956636254938 1101 37 1.504494834850945 1101 38 1.530829036334147 1101 39 1.42326710711653 1101 40 1.4381001317118702 1101 41 1.4445130076807189 1101 42 1.5968207016472746 1101 43 1.582097740648507 1101 44 1.4605551464254276 1101 45 1.5327982401106226 1101 46 1.484552945950174 1101 47 1.5110156987643073 1101 48 1.410764943555396 1101 49 1.5141376907718644 1101 50 1.5309808558509346 1102 1 0.7452708020191743 1102 2 0.7335176674534196 1102 3 0.71762015212067 1102 4 0.7809179750583439 1102 5 0.7157494134969937 1102 6 0.7371120469655352 1102 7 0.8070507616338887 1102 8 0.8252542664848619 1102 9 1.025345981508785 1102 10 1.0115197653474226 1102 11 0.8001751128408188 1102 12 0.7934645529698017 1102 13 0.8385860471078814 1102 14 1.1683236479155 1102 15 1.0735538385583254 1102 16 1.2197469370781562 1102 17 1.350904784515734 1102 18 1.3876146278420975 1102 19 1.2303168731649166 1102 20 1.0545161770669722 1102 21 1.0023516185246208 1102 22 1.2108819043839532 1102 23 1.3887898277222206 1102 24 1.2127945846104495 1102 25 1.1933863151074036 1102 26 1.106875298541219 1102 27 1.1068210265099978 1102 28 1.0978092508365052 1102 29 0.9326630437812753 1102 30 1.1438897921103788 1102 31 1.163745912149345 1102 32 1.1343829965922723 1102 33 0.8394751248733243 1102 34 0.7859544914358452 1102 35 0.9073054448921525 1102 36 0.9711420277665823 1102 37 0.93654909117285 1102 38 1.1216352696767231 1102 39 1.0857244106237047 1102 40 1.0878412071348649 1102 41 1.0682025659656915 1102 42 1.0628381243129255 1102 43 1.111407578060657 1102 44 0.9619741164852371 1102 45 1.0252365421642438 1102 46 0.9890738619997101 1102 47 1.0641648153059684 1102 48 1.0759670472230418 1102 49 1.0931832005055035 1102 50 0.9916140755701832 1103 1 0.2893086329249961 1103 2 0.35455138579538215 1103 3 0.41671992019866266 1103 4 0.30820936920270015 1103 5 0.3175982384520353 1103 6 0.3563365720797478 1103 7 0.44885391094194915 1103 8 0.5156638680266994 1103 9 0.6013631457205193 1103 10 0.6646670901977032 1103 11 0.6305930975170924 1103 12 0.6469824458493747 1103 13 0.655164829308454 1103 14 0.8918372021125336 1103 15 0.6711372541597314 1103 16 0.9032956412686062 1103 17 0.9471147445637627 1103 18 0.6887306559822548 1103 19 0.6822399547771738 1103 20 0.7729450684336072 1103 21 0.7699921668859773 1103 22 0.8524830749341632 1103 23 0.9992882202639137 1103 24 0.8058014948036245 1103 25 0.7174587286779754 1103 26 0.7365743119908146 1103 27 0.7188100385404539 1103 28 0.7728402629069535 1103 29 0.7201813092445946 1103 30 0.8706052988933664 1103 31 0.8341352329106329 1103 32 0.8384176706148097 1103 33 0.8457152525011438 1103 34 0.7560208737662748 1103 35 0.7352952141194322 1103 36 0.7649699958342637 1103 37 0.7269173703804839 1103 38 0.665520702123402 1103 39 0.7739891765713693 1103 40 0.8212866298735548 1103 41 0.9664210401702036 1103 42 0.8370396092184933 1103 43 0.8363501421293762 1103 44 0.7823048268311155 1103 45 0.8377422621864099 1103 46 0.8473995401891052 1103 47 0.7345041900122489 1103 48 0.8136406128121685 1103 49 0.8001924486129575 1103 50 0.8547868781916677 1104 1 0.49939252960599845 1104 2 0.5161048962091392 1104 3 0.5525532732350555 1104 4 0.5515161342951913 1104 5 0.487592857919978 1104 6 0.5850291539108028 1104 7 0.5267812047452054 1104 8 0.559558497782831 1104 9 0.8055765432453441 1104 10 0.8250302697215162 1104 11 0.7871739291479756 1104 12 0.7394843037943843 1104 13 0.658605462294183 1104 14 0.9119894353847968 1104 15 0.8397301875146255 1104 16 0.8482677193745047 1104 17 1.018204065869071 1104 18 1.171555288339185 1104 19 0.8894909246468359 1104 20 0.902948499960587 1104 21 0.9066052817092114 1104 22 0.8506240116451735 1104 23 1.0754251452195476 1104 24 0.9399019906177344 1104 25 1.0263733181631878 1104 26 0.8334203744573827 1104 27 0.7842162527354688 1104 28 0.9603133043325727 1104 29 1.017061431373726 1104 30 1.1944214700121805 1104 31 1.1137486078142231 1104 32 0.9567475202346927 1104 33 0.9371558612041824 1104 34 0.9075028205475704 1104 35 0.8570982866460639 1104 36 0.8787521136806973 1104 37 0.7722454773981156 1104 38 0.8570695604856837 1104 39 0.7687573759654498 1104 40 0.8223095380319663 1104 41 0.9195624849005739 1104 42 1.0417054315292944 1104 43 0.8727788043897959 1104 44 0.8927995162715376 1104 45 0.8456234316661408 1104 46 1.0077210237845513 1104 47 1.030549914310459 1104 48 1.0029979587401208 1104 49 0.9232123712789893 1104 50 0.9997343860531842 1105 1 0.6538888472533841 1105 2 0.7283833967893756 1105 3 0.7128683017309996 1105 4 0.6671328202510765 1105 5 0.5811239988532364 1105 6 0.5836053695951762 1105 7 0.623552178634398 1105 8 0.739677829662682 1105 9 0.8643852206355405 1105 10 1.0233794715343265 1105 11 0.9038644041177761 1105 12 0.8581669158688428 1105 13 0.889750010821281 1105 14 1.0930579308366504 1105 15 0.9461737163336821 1105 16 1.0398890172898518 1105 17 0.9441839553198008 1105 18 1.243372851651948 1105 19 0.9520049786099989 1105 20 0.9307785415564211 1105 21 0.890680339650892 1105 22 0.9829921646283424 1105 23 1.3183816845607481 1105 24 1.0618693927472194 1105 25 1.1547146941683124 1105 26 1.0812182557547487 1105 27 1.1209853628918935 1105 28 1.209779623829668 1105 29 1.1051855266802875 1105 30 1.27383062776547 1105 31 1.3244359621224433 1105 32 1.117492562993803 1105 33 1.2182425264922685 1105 34 1.0281872312176574 1105 35 1.1285007486744547 1105 36 1.126103384766708 1105 37 1.0442669760854741 1105 38 1.1324384541312185 1105 39 1.140972743377148 1105 40 1.2686975740518704 1105 41 1.2963377254288417 1105 42 1.2544271140498964 1105 43 1.2786356391700409 1105 44 1.204543728609913 1105 45 1.1345689407012713 1105 46 1.1356135689937084 1105 47 1.2882766137939932 1105 48 1.3931253018659469 1105 49 1.3731538880925562 1105 50 1.2785425367362961 1106 1 0.2711002832988463 1106 2 0.3125778205262293 1106 3 0.388797701441689 1106 4 0.3909405939422399 1106 5 0.3648601746876281 1106 6 0.2647702612429015 1106 7 0.320863301936285 1106 8 0.34856899713703626 1106 9 -1.0389693702015848 1106 10 -0.6063106687235091 1106 11 0.1511972430426738 1106 12 0.37094855784790326 1106 13 0.3561586251097353 1106 14 0.627243957865943 1106 15 0.5151407591599764 1106 16 0.619271413389761 1106 17 0.694154016714208 1106 18 -1.2190534841291054 1106 19 -0.07762470535336519 1106 20 0.2323824478205978 1106 21 0.39955907613749986 1106 22 0.47438995971084097 1106 23 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0.8540314792361201 1107 11 0.7322431474070967 1107 12 0.8649771476910502 1107 13 0.7201779323392117 1107 14 0.947782584653666 1107 15 0.8057609568678927 1107 16 0.7537479965078475 1107 17 1.1436983644294614 1107 18 1.0859091672918737 1107 19 0.9585007840512887 1107 20 0.9280269407877029 1107 21 0.7961349031796843 1107 22 0.896938858448415 1107 23 1.0650404157586735 1107 24 0.9513088893068371 1107 25 1.0509703035793407 1107 26 0.9440846443486492 1107 27 0.8922398103253784 1107 28 0.8951660364743219 1107 29 0.7972102174415454 1107 30 1.0448442565384397 1107 31 1.0930040312982214 1107 32 1.0760938357016414 1107 33 0.996501317653923 1107 34 0.9827082458613887 1107 35 0.909328714873034 1107 36 1.0234348431014553 1107 37 0.9848255547219154 1107 38 0.9383703713152443 1107 39 0.9104086627533263 1107 40 1.0005304582706103 1107 41 0.8771271879600633 1107 42 0.8954652166310595 1107 43 0.8880744785720722 1107 44 0.9280184561769289 1107 45 0.9794969760645458 1107 46 1.0167064433072746 1107 47 1.0376388351475079 1107 48 0.9645878694375476 1107 49 0.9589832029439229 1107 50 1.090787492150696 1108 1 -0.11567328627596751 1108 2 -0.03951521471595498 1108 3 -0.10606278814088554 1108 4 -0.18177748677764072 1108 5 -0.08985828804491547 1108 6 -0.06198040273148564 1108 7 -0.008239165380409474 1108 8 -0.21203435547883842 1108 9 0.0769406991670607 1108 10 -0.18180326798616164 1108 11 -0.05335120889649403 1108 12 -0.16680164528580832 1108 13 -0.04256375476850138 1108 14 -0.19327436661439634 1108 15 -0.13407058176510134 1108 16 -0.07250341440135344 1108 17 -0.19933009034635063 1108 18 0.19162619522280977 1108 19 0.07576391370118074 1108 20 -0.02377993181509197 1108 21 0.04525461204669057 1108 22 -0.0032339518562594094 1108 23 -0.21891292054248623 1108 24 -0.10949403424005766 1108 25 -0.05825418205709809 1108 26 -0.04046046183754015 1108 27 -0.10150253917962715 1108 28 -0.059106388787718345 1108 29 -0.07803933619267411 1108 30 -0.0186878898433136 1108 31 0.21989277984112476 1108 32 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-1.31305277400255 2216 19 -1.5618146772263017 2216 20 -1.6160675725365934 2216 21 -1.7460333006645143 2216 22 -1.9387166257804083 2216 23 -3.721371711614591 2216 24 -2.4299825916000337 2216 25 -2.0432719443115595 2216 26 -2.140789420116974 2216 27 -2.1136486830621806 2216 28 -2.3837912994954884 2216 29 -2.1054460304563456 2216 30 -1.9326109225616825 2216 31 -1.7484190721102344 2216 32 -1.9587977235331024 2216 33 -1.9513446607297524 2216 34 -2.0636562989216785 2216 35 -2.369135786343442 2216 36 -2.1405789373191126 2216 37 -2.041215695304846 2216 38 -2.201350287179771 2216 39 -2.2095857746121688 2216 40 -2.2598150164705366 2216 41 -2.275248913642333 2216 42 -2.537217066429914 2216 43 -2.514294939499372 2216 44 -2.5201233095209936 2216 45 -2.6026574533076854 2216 46 -2.562034214837052 2216 47 -2.509017173837922 2216 48 -2.594920370707648 2216 49 -2.813605618588575 2216 50 -3.012725375176771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203878.0 3 10204.0 4 19192.0 5 14831.0 6 12016.0 7 10034.0 8 9016.0 9 8061.0 10 7704.0 11 7417.0 12 7645.0 13 7997.0 14 8201.0 15 8786.0 16 9414.0 17 10376.0 18 11470.0 19 12890.0 20 16188.0 21 21421.0 22 26723.0 23 30788.0 24 32530.0 25 34858.0 26 37439.0 27 42363.0 28 48920.0 29 54285.0 30 62839.0 31 74900.0 32 89663.0 33 110809.0 34 139117.0 35 176939.0 36 233120.0 37 316803.0 38 498147.0 39 918960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7922230403137 17.590688032212203 12.650807787247304 24.966281140226794 2 15.735733185072245 19.986954380107257 39.097710855588694 25.179601579231804 3 18.709847017172812 25.04122587232271 28.956592162030088 27.29233494847439 4 12.591428744054554 16.370762662436135 37.41201095679317 33.62579763671614 5 14.037812852493264 37.48815861931371 34.25907729508915 14.214951233103879 6 32.31963395213565 36.72025300367095 17.56168459772865 13.398428446464752 7 28.396597750970585 31.851203333596462 21.857022057328482 17.895176858104474 8 26.511453068540774 34.725748074657794 19.904089503703855 18.858709353097577 9 26.925231149147454 15.69128838568049 18.776078010725307 38.60740245444674 10 14.55261320186201 28.84991005459529 33.75373651005849 22.843740233484215 11 35.012810560399224 23.096926063569565 23.547209486752255 18.34305388927896 12 23.573929329175687 24.728328325693766 30.243530993031975 21.454211352098575 13 28.169265976574508 21.97614145732821 25.69695081029803 24.15764175579925 14 22.609164023873067 20.921564358354438 26.735630520108955 29.733641097663536 15 23.816020140706176 28.719409896691246 23.579964460040575 23.884605502562007 16 23.86991396229846 27.901746057477705 24.938640636617976 23.289699343605854 17 22.872195794330878 27.616473415515554 26.273511774694974 23.237819015458598 18 22.887004323268638 27.2921623371671 26.966160067114597 22.85467327244967 19 23.74271300515488 26.942231611889838 26.90505642374092 22.409998959214363 20 23.87498478243898 26.513672473817568 26.91113871625702 22.70020402748643 21 24.107228585196335 26.261324061613784 26.86406759006979 22.76737976312009 22 23.887137002932064 26.06690652366572 26.742749330692494 23.30320714270973 23 23.840487968136355 26.085801781787154 26.643780449032857 23.429929801043638 24 23.282484867026312 26.29281096649767 26.846572685375 23.578131481101018 25 23.622649455436676 26.11385505609542 26.791893449656794 23.47160203881111 26 23.285869451740865 26.571338061474208 26.93206595466357 23.210726532121353 27 23.507397060319093 26.42826376965919 26.682479765016957 23.381859405004757 28 23.58214679658263 26.39142145372529 26.678595607991575 23.347836141700505 29 23.680216150371926 26.42909151390816 26.662694018112642 23.227998317607266 30 23.717204213916595 26.421117080922667 26.62998238568266 23.231696319478072 31 23.82892392065749 26.06621207652859 26.586942906246318 23.517921096567605 32 23.394604082204022 26.420722219530184 26.70254852096528 23.48212517730051 33 23.677660068950292 26.22393351674614 26.611397556529848 23.487008857773716 34 23.83949260062516 26.242779164757657 26.595282726577818 23.322445508039365 35 23.715189911281012 26.365422925752434 26.608128884820857 23.311258278145697 36 23.7602085122562 26.355364066335994 26.466913217512467 23.417514203895337 37 23.804916691945984 26.11837779201796 26.600120742380156 23.476584773655905 38 23.766204787961122 26.30339706075012 26.639651458348663 23.290746692940093 39 23.872197089494573 26.383004428197882 26.388028315586254 23.35677016672129 40 23.77448253669764 26.212747606623815 26.78096136983778 23.23180848684077 41 23.66211992034696 26.22084968225208 26.661070579183093 23.455959818217867 42 23.84662819601848 26.252778236331313 26.535087719298247 23.365505848351958 43 23.66689200441666 26.155553608807487 26.54068083892616 23.63687354784969 44 23.746225281197862 26.32819273938444 26.422074854542522 23.503507124875174 45 23.75385745138064 26.355680563408413 26.23700815158973 23.653453833621217 46 23.841233848553127 26.26894401469596 26.148421092916564 23.741401043834344 47 23.686436231516204 26.38900722864047 26.290558520776024 23.6339980190673 48 23.59052592298455 26.478605225262292 26.365579386000583 23.565289465752574 49 23.8748597191003 26.237230438645256 26.238217226748656 23.649692615505792 50 23.97130824648716 26.30512944328251 26.223742455931575 23.499819854298753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2052.0 1 1729.5 2 1407.0 3 1405.0 4 1403.0 5 1437.5 6 1472.0 7 1396.0 8 1320.0 9 1478.5 10 1637.0 11 2040.0 12 2443.0 13 4207.0 14 5971.0 15 8417.0 16 10863.0 17 11984.5 18 13106.0 19 14278.5 20 15451.0 21 17889.0 22 20327.0 23 25056.0 24 29785.0 25 36082.5 26 42380.0 27 49458.0 28 56536.0 29 63893.5 30 71251.0 31 79776.0 32 88301.0 33 98601.5 34 108902.0 35 119578.0 36 130254.0 37 139085.5 38 147917.0 39 157777.5 40 167638.0 41 178117.5 42 188597.0 43 195959.0 44 203321.0 45 211283.5 46 219246.0 47 225972.0 48 232698.0 49 234259.5 50 235821.0 51 237479.0 52 239137.0 53 233512.5 54 227888.0 55 219522.5 56 211157.0 57 197024.5 58 182892.0 59 166585.5 60 150279.0 61 132159.0 62 114039.0 63 97628.5 64 81218.0 65 68244.5 66 55271.0 67 45155.5 68 35040.0 69 27961.0 70 20882.0 71 16361.0 72 11840.0 73 9541.0 74 7242.0 75 5607.5 76 3973.0 77 3067.0 78 2161.0 79 1685.5 80 1210.0 81 933.0 82 656.0 83 516.0 84 376.0 85 295.0 86 214.0 87 174.0 88 134.0 89 110.5 90 87.0 91 65.5 92 44.0 93 35.5 94 27.0 95 22.5 96 18.0 97 22.0 98 26.0 99 30.0 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06330052146718534 2 0.06853073452514448 3 0.0648546419186932 4 0.07860263052818577 5 0.08353397426854724 6 0.06333040839894512 7 0.046862708999313794 8 0.05956465499721454 9 0.05651618795771836 10 0.06721570952771476 11 0.05463331125685308 12 0.07131021917880276 13 0.06682717941483778 14 0.06754446577707218 15 0.0796486731397776 16 0.07549438962517005 17 0.08622379812692621 18 0.07199761860927738 19 0.0692181339556191 20 0.08269714017927376 21 0.08162121063592218 22 0.07660020610028141 23 0.07313332201614851 24 0.06237402658263258 25 0.0783934220058674 26 0.08909294357586378 27 0.08141200211360382 28 0.10080862082569224 29 0.09223107141063927 30 0.07893138677754319 31 0.07668986689556072 32 0.08218906233935774 33 0.07967856007153737 34 0.06419712941997835 35 0.08380295665438513 36 0.09034819470977398 37 0.09832800548963162 38 0.07896127370930296 39 0.05738290897875158 40 0.06371893851182207 41 0.058010534545706684 42 0.06264300896847048 43 0.03989905389928821 44 0.03957029764993078 45 0.043126842529342985 46 0.040407131739204245 47 0.03153071300655361 48 0.047101804453391925 49 0.052630886828948724 50 0.04270842548470626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3345944.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.497970387189405 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87941497133315 50.721807263957274 2 11.588195078504407 14.716537358716938 3 3.786345837233577 7.212758275449467 4 1.6694133012576522 4.240174254689541 5 0.8584329043250438 2.7254373569110313 6 0.5186214060614982 1.9758844010553327 7 0.3460813855228733 1.5382825898642225 8 0.2286542268514643 1.1615263460415977 9 0.18514616309989493 1.0580765023636982 >10 0.8651992070275262 9.667754446687312 >50 0.05190081049652981 2.251923929290899 >100 0.02174352882601639 2.2113351822561396 >500 6.620284694085201E-4 0.28519416733944003 >1k 1.8915099074633465E-4 0.23330792537712286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3359 0.10039020378105551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.97738635195329E-5 2 0.0 0.0 0.0 0.0 5.97738635195329E-5 3 0.0 0.0 0.0 0.0 5.97738635195329E-5 4 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 5 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 6 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 7 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 8 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 9 0.0 0.0 0.0 2.988693175976645E-5 5.97738635195329E-5 10 0.0 0.0 0.0 5.97738635195329E-5 5.97738635195329E-5 11 0.0 0.0 0.0 5.97738635195329E-5 5.97738635195329E-5 12 0.0 0.0 0.0 8.966079527929936E-5 5.97738635195329E-5 13 0.0 0.0 0.0 8.966079527929936E-5 8.966079527929936E-5 14 0.0 2.988693175976645E-5 0.0 8.966079527929936E-5 8.966079527929936E-5 15 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 8.966079527929936E-5 16 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 8.966079527929936E-5 17 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 8.966079527929936E-5 18 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 8.966079527929936E-5 19 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 1.195477270390658E-4 20 0.0 2.988693175976645E-5 0.0 1.195477270390658E-4 1.4943465879883227E-4 21 0.0 2.988693175976645E-5 0.0 1.793215905585987E-4 1.4943465879883227E-4 22 0.0 2.988693175976645E-5 0.0 2.6898238583789806E-4 1.793215905585987E-4 23 0.0 2.988693175976645E-5 0.0 2.9886931759766453E-4 1.793215905585987E-4 24 0.0 2.988693175976645E-5 0.0 3.2875624935743095E-4 1.793215905585987E-4 25 0.0 2.988693175976645E-5 0.0 3.586431811171974E-4 1.793215905585987E-4 26 0.0 2.988693175976645E-5 0.0 4.184170446367303E-4 2.390954540781316E-4 27 0.0 2.988693175976645E-5 0.0 5.080778399160296E-4 2.390954540781316E-4 28 0.0 2.988693175976645E-5 0.0 8.069471575136942E-4 2.390954540781316E-4 29 0.0 2.988693175976645E-5 0.0 0.0014046857927090231 2.390954540781316E-4 30 0.0 2.988693175976645E-5 0.0 0.0022116329502227176 2.390954540781316E-4 31 0.0 2.988693175976645E-5 0.0 0.004423265900445435 2.390954540781316E-4 32 0.0 2.988693175976645E-5 0.0 0.0067245596459474515 2.390954540781316E-4 33 0.0 2.988693175976645E-5 0.0 0.01019144373008036 2.390954540781316E-4 34 0.0 2.988693175976645E-5 0.0 0.01315024997429724 2.390954540781316E-4 35 0.0 2.988693175976645E-5 0.0 0.016975777239547345 2.390954540781316E-4 36 0.0 2.988693175976645E-5 0.0 0.022445085751584604 2.6898238583789806E-4 37 0.0 5.97738635195329E-5 0.0 0.029737497100967618 2.6898238583789806E-4 38 0.0 5.97738635195329E-5 0.0 0.03900244594649522 2.6898238583789806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1990 0.0 27.088228 1 GTATCAA 4450 0.0 23.930563 1 TCAACGC 6040 0.0 17.521154 4 TATCAAC 5985 0.0 17.497576 2 CAACGCA 6080 0.0 17.441027 5 ATCAACG 6080 0.0 17.440245 3 CGTGCCG 285 0.0 16.983957 10 GCACCGT 430 0.0 16.882183 6 AACGCAG 6365 0.0 16.65834 6 CGCACGA 300 0.0 15.401131 35 GTACATG 7675 0.0 15.308422 1 ACGCAGA 7165 0.0 14.676882 7 GGACCGA 305 0.0 14.424915 6 TACATGG 7950 0.0 14.390348 2 CGCAGAG 7510 0.0 13.856588 8 GTGCCGA 335 0.0 13.791645 11 ACATGGG 8090 0.0 13.705381 3 AGAGTAC 7140 0.0 13.434758 11 GAGTACT 3960 0.0 13.38967 12 GTATTAT 685 0.0 12.848043 1 >>END_MODULE