FastQCFastQC Report
Mon 6 Feb 2017
SRR2077467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345944
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40090.11981670942490369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.027.1719071
GTATCAA54850.024.5501171
CGTACGC708.1457064E-418.85084242
TATACCG1557.221388E-1018.4545485
TATCAAC77950.017.0759752
ATCAACG80300.016.8235873
TCAACGC80700.016.7674624
CAACGCA82100.016.3217185
AACGCAG84350.015.7540296
GCGATAC3100.015.61538614
GCGTTAA1704.5154593E-815.52445340
TGCGATA3100.014.90559613
GTTCGAC900.004357406414.66856111
CGAATCG3400.014.23351437
CGTACAA3900.014.10354125
TAGCCCG1100.001017436214.0022275
CGTTAAT2052.9451257E-813.94655641
GTACATG79950.013.7603751
GGACCGT2256.728442E-913.6894286
AGCGTAC3550.013.634747523
Produced by FastQC (version 0.11.5)