FastQCFastQC Report
Mon 6 Feb 2017
SRR2077466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463070
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.024.7056061
GGTATCA14350.023.6091671
TCAACGC45800.018.301974
TATCAAC45900.018.2126852
CAACGCA46300.018.1507345
ACCGTCG851.4316461E-418.1175638
ATCAACG46300.018.008193
AACGCAG48650.017.2732756
GTACATG64000.015.5027381
TTGCGCG1004.947219E-415.39961618
TACATGG63200.015.31576252
CGCACGA1201.18103555E-414.66779135
ACGCAGA57100.014.3326837
GCACCGT3400.014.234366
ACATGGG65500.014.1736923
CGCAGAG57800.014.0449188
TACCGAT2401.1277734E-913.7499367
GTACTAT4050.013.5799081
AGAGTAC55500.013.35876811
CGTGCCG2155.4706106E-813.30280410
Produced by FastQC (version 0.11.5)