FastQCFastQC Report
Mon 6 Feb 2017
SRR2077466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463070
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36350.024.576161
GGTATCA16500.021.336761
GACCGTA808.990719E-519.2507467
TATCAAC51850.017.101032
ATCAACG52250.016.928693
CAACGCA52800.016.836385
TCAACGC52800.016.7940244
AACGCAG53700.016.5112176
TCGCACG850.002978731615.53127518
GGCGCGA900.00435782414.6681310
CGTATTA1402.7568645E-514.14397928
TTAGTCG950.006254104413.89076239
ACGCAGA64300.013.7890267
GTACATG54800.013.7321331
TACATGG55150.013.4047812
GCACCGT2805.820766E-1113.3579316
CGCAGAG66300.013.3069718
GTCCTAT4500.013.2021211
GTCGCGT1000.00877456613.19756435
AGAGTAC64650.012.76255211
Produced by FastQC (version 0.11.5)