FastQCFastQC Report
Mon 6 Feb 2017
SRR2077465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064533
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.10641099312684837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.026.243011
GTATCAA46350.026.0121381
TATCAAC60650.019.4424442
ATCAACG61150.019.1392523
TCAACGC61200.019.0170174
CAACGCA61800.018.6528635
TGCGATA1901.8189894E-1218.52788513
AACGCAG64400.018.0361426
GCGATAC1852.1827873E-1117.83847214
GTGCGAT2353.6379788E-1215.91624112
ACGCAGA74050.015.5676537
CGCAGAG76700.015.0300318
GTACATG76450.014.878471
CGATACT2254.274625E-1014.66719115
TACATGG77100.014.4952672
AGAGTAC73250.014.41760411
CGTGCCG3250.014.2168210
GTATAAC4250.013.9771981
TATACTG7650.013.8032875
ACATGGG80550.013.7376433
Produced by FastQC (version 0.11.5)