FastQCFastQC Report
Mon 6 Feb 2017
SRR2077465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064533
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.12990560062495657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA250.00235462735.1961235
GGTATCA21750.029.4365251
GTATCAA52650.026.117671
TATCAAC76950.017.555192
ATCAACG78700.017.2489743
TCAACGC79800.016.9839124
CAACGCA80250.016.9171965
TCGATCG1053.788903E-516.75595741
AACGCAG82250.016.5312396
GTCTTAC3650.015.0695381
CGTGCCG2551.2732926E-1114.66792410
CGGCACG2900.014.41526422
GTGCCGA2753.6379788E-1214.40146811
CGCACGA2153.5561243E-914.32400235
ACGCAGA95350.014.1215887
CGTTATA950.006240525713.8954742
GCGATAC2551.891749E-1013.8057814
TAGGACT6100.013.7060934
GTACATG76550.013.6521551
CGCAGAG99000.013.5567188
Produced by FastQC (version 0.11.5)