FastQCFastQC Report
Mon 6 Feb 2017
SRR2077464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3579048
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.10737492204630952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60200.021.6721521
GGTATCA29800.019.7861981
TATCAAC76800.016.586052
ATCAACG76700.016.5206993
TCAACGC76750.016.4824264
CAACGCA77300.016.165475
AACGCAG79500.015.8556016
TACCGCG1201.18183656E-414.66697117
GTACATG85150.013.82333851
TACATGG83200.013.64432052
GAGTACT46000.013.63109812
AGAGTAC83600.013.5000911
ACGCAGA92300.013.3957267
GTATAAA14650.013.0654811
CGCAGAG94450.012.9976228
ACATGGG86950.012.9286323
ATACCGT1557.1840506E-512.7733866
CGTATAC1751.6000242E-512.5708093
ACTATAC6500.012.522463
GTGTAGC13450.012.431831
Produced by FastQC (version 0.11.5)