FastQCFastQC Report
Mon 6 Feb 2017
SRR2077464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3579048
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39160.10941457057854491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69150.022.0177271
GGTATCA34450.021.0118941
ATCAACG94000.016.1032333
TATCAAC94100.016.0386852
TCAACGC95950.015.8222684
CAACGCA96950.015.6365915
AACGCAG99900.015.0643066
TAGCGCG900.004360857414.66685429
TACATGG78700.014.0054562
GTACATG80800.013.9417471
GAGTACT71400.013.1588712
ACATGGG84050.013.0359933
TATAACG1701.1866043E-512.9415232
ACGCAGA115500.012.9148117
AGAGTAC107250.012.903810511
CATACCG1751.597399E-512.5728195
CGCAGAG117950.012.571948
AGTACTT75100.012.33494313
CCTATAC4300.012.2799123
GTATAAT8650.011.9547441
Produced by FastQC (version 0.11.5)