FastQCFastQC Report
Mon 6 Feb 2017
SRR2077463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578698
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.11023562200554503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65000.019.4625171
GCGCAAT1501.8400715E-716.13369235
TTATCGT1704.4976332E-815.529544
GGTATCA37450.015.3919621
TATCAAC80650.015.2762232
CGCAATA1301.3703289E-515.23089436
TCAACGC81050.015.1191884
ATCAACG81350.015.1168843
CAACGCA81750.015.0435495
ATAGCCC8850.014.9147513
GTACATG77300.014.857151
AACGCAG83050.014.7275696
CGTGCCG1057.160191E-414.66760810
TACATGG75950.014.541472
GTATAGC9600.014.4382261
CGGGGTA4000.014.2961240
CGTTACG950.00623975413.89582424
GAGTACT46750.013.8830512
AGAGTAC78150.013.73818211
TAGCCCT15450.013.5276194
Produced by FastQC (version 0.11.5)