FastQCFastQC Report
Mon 6 Feb 2017
SRR2077463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578698
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.13256217764114211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74450.022.3421961
GGTATCA37500.018.7166521
TATCAAC102150.016.06762
ATCAACG104450.015.7352943
TCAACGC104450.015.6723194
CAACGCA104300.015.6324465
CGGGGTA4800.015.57910740
AACGCAG107750.015.1506456
AAACGGG5100.015.09542137
TACACCG2305.9480953E-1014.350155
AGAGTAC113050.013.3703511
GAGTACT79850.013.19901512
ACGCAGA123400.013.0863737
CGCAGAG125050.012.896298
AACGGGG5800.012.89413538
TACCGCG1557.1773655E-512.77465629
GGACCGA2259.914038E-812.7117486
AGTACTT84050.012.67052513
GTACATG78500.012.55674651
GTATTAT7600.012.44868851
Produced by FastQC (version 0.11.5)