FastQCFastQC Report
Mon 6 Feb 2017
SRR2077462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724962
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.12214889708941996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62200.021.8245641
GGTATCA30900.020.790991
TACCGTA1154.2789015E-617.2168887
TCAACGC76850.017.1187924
TATCAAC78450.017.050312
ATCAACG77400.017.0246543
CAACGCA78100.016.8161835
AACGCAG80100.016.3130326
ATAACGC1501.8420542E-716.132213
GTACATG82950.015.4633271
TACATGG82250.015.2194162
TAAGGCG2551.2732926E-1114.6660395
GTACCGT2153.5561243E-914.3241996
ACGCAGA91250.014.2724587
ATAGCCC8500.014.23430253
ACATGGG85750.014.084153
AGAGTAC85500.014.02429911
CGCAGAG93300.013.8413398
GAGTACT51850.013.36652212
CAGAGTA95350.012.96832710
Produced by FastQC (version 0.11.5)