FastQCFastQC Report
Mon 6 Feb 2017
SRR2077462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724962
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53610.14392093127392977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73150.022.8599341
GGTATCA34650.022.7964341
TATCAAC100900.016.3757482
ATCAACG102350.016.2514513
TCAACGC103050.016.0770054
CAACGCA103250.016.0465135
AACGCAG105350.015.7247466
TATACCG1503.2505868E-614.6684575
TACACCG1851.2659439E-714.2720135
GTACATG74950.014.2378961
TACATGG73900.014.022612
TAGTACT6900.013.7112674
ACCGTCG1150.001424104713.392228
ACGCAGA122700.013.3766287
CCTATAC5600.013.3582363
CGCAGAG124950.013.0653198
AGAGTAC117800.012.96218511
ACATGGG80050.012.8904983
GTATAGC9450.012.8057971
GTCCTAC4250.012.4250461
Produced by FastQC (version 0.11.5)