##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077461_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252612 Sequences flagged as poor quality 0 Sequence length 50 %GC 28 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.082466391145314 30.0 10.0 31.0 7.0 34.0 2 23.916302471774895 30.0 16.0 33.0 7.0 34.0 3 24.999798109353474 30.0 22.0 33.0 7.0 34.0 4 26.462666064953368 33.0 17.0 37.0 7.0 37.0 5 29.201653128117428 35.0 28.0 37.0 7.0 37.0 6 27.709982106946622 35.0 22.0 37.0 7.0 37.0 7 28.38222253891343 35.0 25.0 37.0 7.0 37.0 8 27.458786597627984 35.0 21.0 37.0 7.0 37.0 9 27.850058587873892 34.0 17.0 39.0 2.0 39.0 10 28.74642534796447 34.0 23.0 39.0 2.0 39.0 11 28.649323864266147 35.0 23.0 39.0 2.0 39.0 12 29.5378248064225 35.0 27.0 39.0 2.0 39.0 13 29.402063243234686 35.0 27.0 39.0 2.0 39.0 14 30.2198391208652 35.0 27.0 40.0 2.0 41.0 15 30.252668123446234 35.0 27.0 40.0 2.0 41.0 16 30.30215904232578 35.0 28.0 40.0 2.0 41.0 17 30.104155780406316 35.0 28.0 40.0 2.0 41.0 18 29.919485218437764 35.0 28.0 40.0 2.0 41.0 19 29.819137649834527 34.0 28.0 40.0 2.0 41.0 20 29.612805409085873 34.0 27.0 40.0 2.0 41.0 21 29.432010355802575 34.0 27.0 40.0 2.0 41.0 22 29.40834560511773 34.0 27.0 40.0 2.0 41.0 23 28.826326540306873 33.0 25.0 40.0 2.0 41.0 24 28.90301331686539 33.0 25.0 40.0 2.0 41.0 25 28.74026174528526 33.0 25.0 40.0 2.0 41.0 26 28.47828685889823 33.0 25.0 40.0 2.0 41.0 27 28.19397336626922 33.0 25.0 39.0 2.0 41.0 28 28.105363165645336 33.0 25.0 39.0 2.0 41.0 29 27.93209744588539 33.0 25.0 39.0 2.0 41.0 30 27.58824996437224 33.0 25.0 39.0 2.0 41.0 31 27.376288537361646 33.0 25.0 38.0 2.0 41.0 32 27.092952828844236 32.0 25.0 38.0 2.0 40.0 33 27.02390226909252 32.0 25.0 38.0 2.0 40.0 34 26.812930502113915 32.0 25.0 38.0 2.0 40.0 35 26.71114594714424 31.0 25.0 38.0 2.0 40.0 36 26.471038588823966 31.0 23.0 37.0 2.0 40.0 37 26.301094168131364 30.0 22.0 37.0 2.0 40.0 38 26.17541526134942 30.0 22.0 37.0 2.0 40.0 39 26.009888683039602 30.0 22.0 37.0 2.0 40.0 40 25.96491061390591 30.0 22.0 37.0 2.0 40.0 41 25.80585641220528 30.0 22.0 37.0 2.0 40.0 42 25.541241112852912 30.0 21.0 36.0 2.0 40.0 43 25.479949487751966 30.0 21.0 36.0 2.0 40.0 44 25.34779028708058 30.0 20.0 36.0 2.0 40.0 45 25.196534606432 29.0 20.0 36.0 2.0 40.0 46 24.842343198264533 28.0 18.0 36.0 2.0 40.0 47 24.62601143255269 27.0 12.0 36.0 2.0 40.0 48 24.20646683451301 25.0 2.0 35.0 2.0 40.0 49 24.08998384874828 27.0 2.0 35.0 2.0 40.0 50 23.944852184377623 27.0 2.0 35.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.0521364406399663 1101 2 1.8999498361792568 1101 3 1.4799851552299472 1101 4 1.7937124954611967 1101 5 1.4409873122220986 1101 6 1.8336097321374467 1101 7 1.5180121605827495 1101 8 1.9959645734579787 1101 9 2.3877607559457807 1101 10 2.294051457026928 1101 11 2.2552463057768826 1101 12 1.9352148171378154 1101 13 1.9625364306275657 1101 14 2.446723078790498 1101 15 2.032087290169553 1101 16 2.101071918187209 1101 17 2.0869856059786294 1101 18 2.3969857259285092 1101 19 1.8078949679035894 1101 20 2.154879105901184 1101 21 2.1963921827948383 1101 22 2.026534732388896 1101 23 2.057237738939506 1101 24 2.099818438633818 1101 25 2.1927297223110322 1101 26 2.18358367472106 1101 27 2.0801947489796753 1101 28 2.4255190192829055 1101 29 2.2474673542631862 1101 30 2.5912438642761977 1101 31 2.634732910418837 1101 32 2.541486664768506 1101 33 2.2452010488550442 1101 34 2.033206111300313 1101 35 2.281079286772318 1101 36 2.193254835561678 1101 37 2.2150699446556885 1101 38 2.112187050021589 1101 39 2.1645863881873026 1101 40 2.328203015021728 1101 41 2.3603346155944926 1101 42 2.524135656998723 1101 43 2.618254773400391 1101 44 2.655972850794509 1101 45 2.7631952860268 1101 46 2.7386982368774433 1101 47 2.889312797169204 1101 48 2.547826306795244 1101 49 2.130762705517867 1101 50 2.158855148638935 1102 1 0.766937326316107 1102 2 0.5730392829572857 1102 3 0.6200245547936234 1102 4 1.4135392676275202 1102 5 0.7522764268715747 1102 6 0.976848311148224 1102 7 1.279343605341957 1102 8 1.6354341446214065 1102 9 2.1424414892335264 1102 10 1.668632226132459 1102 11 1.614523112132911 1102 12 1.5206565155520657 1102 13 1.6617810009769514 1102 14 1.6361177582213209 1102 15 1.749940827989878 1102 16 2.0057233460143777 1102 17 1.4791903534632418 1102 18 1.6124948269021004 1102 19 1.5511140099406475 1102 20 1.2393765172521647 1102 21 1.3443359163105164 1102 22 1.316202929451837 1102 23 1.330511158886754 1102 24 1.1592026983453216 1102 25 1.5033187620687727 1102 26 1.1836080959382151 1102 27 1.3095832417021533 1102 28 1.4144399271009505 1102 29 1.297473866587758 1102 30 1.5919113775450597 1102 31 1.8338228130595837 1102 32 1.6493633213410668 1102 33 1.4770560845100213 1102 34 1.1864248277211402 1102 35 1.6093167261262948 1102 36 1.1371348785430193 1102 37 1.0910264423275322 1102 38 1.0053686461923412 1102 39 1.210864594693117 1102 40 0.9050484449979592 1102 41 1.0904801660487244 1102 42 0.8675549530164375 1102 43 0.7497223257431997 1102 44 0.6945502334912597 1102 45 1.1598691162504977 1102 46 1.2019605858729179 1102 47 1.2771298659719115 1102 48 1.1830303053625322 1102 49 0.8557716599641552 1102 50 0.8187327169369389 1103 1 1.6199435155291937 1103 2 1.4774257649299756 1103 3 1.628399640754914 1103 4 1.9893816882975273 1103 5 1.7325298898836579 1103 6 1.6602160289987005 1103 7 1.8056968816952335 1103 8 2.235321166922219 1103 9 2.4921811493180144 1103 10 2.2517199557719145 1103 11 2.317956651773347 1103 12 1.6106948297283097 1103 13 1.5634904026863516 1103 14 1.4141411396929762 1103 15 1.1082274966213745 1103 16 1.6228026268867595 1103 17 1.738253129767397 1103 18 1.6528229534371235 1103 19 1.6724422347171455 1103 20 1.7952267972403781 1103 21 1.6163174469127348 1103 22 1.653662404842347 1103 23 2.2852709584741397 1103 24 2.220235216274393 1103 25 2.0674704469101144 1103 26 2.017211593334814 1103 27 1.8372627780023798 1103 28 2.0216950602181534 1103 29 1.8587519882107095 1103 30 1.8860954820739906 1103 31 1.9228050313521479 1103 32 1.6452371792838925 1103 33 1.7941304533085258 1103 34 1.8928301720575185 1103 35 1.819799091781075 1103 36 1.7385200833764998 1103 37 1.8222868804845227 1103 38 1.7011639109531238 1103 39 1.8097200814796395 1103 40 1.6264847353997673 1103 41 1.6721188338942916 1103 42 1.6643178963311058 1103 43 2.1319307941585635 1103 44 1.8074542515676946 1103 45 1.6834716312323224 1103 46 1.3292569804106904 1103 47 1.7701792814351194 1103 48 2.005434274318226 1103 49 1.7105953165119523 1103 50 1.4872168509383137 1104 1 0.35173024731592406 1104 2 0.46297725048146177 1104 3 0.40149691385218844 1104 4 0.21567391458975393 1104 5 0.10595276330652936 1104 6 0.47584484462751675 1104 7 0.6589732349715867 1104 8 0.3063427379437904 1104 9 0.9376430947799612 1104 10 0.9824891865411445 1104 11 0.9479932818099783 1104 12 0.5286842477177274 1104 13 0.40516724436319507 1104 14 0.6013613269131639 1104 15 0.5504334388273158 1104 16 0.4685494726336046 1104 17 0.1704875620018278 1104 18 0.3150126156267845 1104 19 0.39730995958487014 1104 20 -0.1705851685715878 1104 21 -0.1486398180445292 1104 22 -0.0026257514458087883 1104 23 0.46862464182782304 1104 24 0.35556285160202705 1104 25 0.3662087456484109 1104 26 0.3501379262611479 1104 27 0.39994084068044344 1104 28 0.3548248933479847 1104 29 0.5040511335098543 1104 30 0.4532505492290575 1104 31 0.2553243638714804 1104 32 0.552848086894798 1104 33 0.27316993234800435 1104 34 0.06865434788169367 1104 35 0.18019795217993462 1104 36 0.3960292408856567 1104 37 0.5426776008752441 1104 38 0.29172150151071463 1104 39 0.6129353846949428 1104 40 0.2839938929089243 1104 41 0.25596091773637397 1104 42 0.3480785800917907 1104 43 0.3331924954202634 1104 44 0.012785956899399764 1104 45 -0.02043557267488083 1104 46 0.04801969917340898 1104 47 0.40835591961175766 1104 48 0.2058819747659264 1104 49 0.07384317975981247 1104 50 -0.003015139293676583 1105 1 1.240837772787085 1105 2 1.2154587756902586 1105 3 0.9442622293447762 1105 4 1.46203361549491 1105 5 0.8220968885415658 1105 6 0.3964360379459819 1105 7 1.521079142532038 1105 8 1.2409485361859538 1105 9 1.4256180827549478 1105 10 1.0469213873369831 1105 11 1.0071563409730366 1105 12 1.3499051143931418 1105 13 1.2692190175058755 1105 14 1.4609966410939315 1105 15 1.311354336111851 1105 16 1.1626768940337548 1105 17 1.3680475777436634 1105 18 1.1297837031251454 1105 19 1.322273884548796 1105 20 1.2986526643026082 1105 21 1.1827039223901217 1105 22 1.517969814604303 1105 23 1.723358123834032 1105 24 1.6939466899858644 1105 25 1.8589281474587231 1105 26 1.6904239029107622 1105 27 1.5577308621068262 1105 28 1.5238965624196545 1105 29 1.749383242478327 1105 30 1.7221954090830103 1105 31 1.6304172844189466 1105 32 1.3787259127726266 1105 33 1.7180079226405418 1105 34 1.808301905711069 1105 35 1.8225303672396223 1105 36 1.5239972324900144 1105 37 1.375770433968075 1105 38 1.584213521275462 1105 39 1.3485818930687223 1105 40 1.5582734853703357 1105 41 2.029256645577558 1105 42 1.9868909461768816 1105 43 1.9364326895695498 1105 44 1.49385202887456 1105 45 1.8593798980496814 1105 46 2.079109821172622 1105 47 2.0548205660764047 1105 48 1.9910715326827564 1105 49 1.6001670242654775 1105 50 1.2131945769160417 1106 1 -0.8878988194408031 1106 2 -0.44973523403953664 1106 3 -0.09867194801880075 1106 4 0.11334362077009885 1106 5 -0.03324570567917107 1106 6 -0.6214336648850356 1106 7 -0.2632095861474042 1106 8 0.1723930587813456 1106 9 -0.5178055149239675 1106 10 0.026421416643586326 1106 11 0.35064441318451145 1106 12 0.1570896287826855 1106 13 0.36213561090602653 1106 14 0.039853390405227174 1106 15 -0.03530617616761589 1106 16 0.14903466907624718 1106 17 -0.27163503171651016 1106 18 -1.1806391911952332 1106 19 -0.34036312383289413 1106 20 -0.49209817093565533 1106 21 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0.7632413948424492 1107 9 0.6491612062980714 1107 10 0.6514350554870134 1107 11 0.8136831474998445 1107 12 0.38847260750608825 1107 13 0.25408869328464334 1107 14 0.7443405698924082 1107 15 0.6464041347980114 1107 16 0.1783581824423166 1107 17 0.5382531297673978 1107 18 1.0513984520126236 1107 19 0.9619009241758363 1107 20 0.76873107074465 1107 21 1.1610467916420824 1107 22 1.0246025757825201 1107 23 0.9989461721493527 1107 24 0.700007296046472 1107 25 0.847527426967094 1107 26 0.7331660092892314 1107 27 0.5737300144696142 1107 28 0.7325212710443623 1107 29 0.41060384006256 1107 30 0.5935028894813996 1107 31 0.567819276366393 1107 32 0.16660470065141197 1107 33 0.1721931313712055 1107 34 0.3358501150774629 1107 35 0.4895996615816465 1107 36 0.6453576902141087 1107 37 0.8023438605415016 1107 38 0.5390556488448617 1107 39 0.3077257794853345 1107 40 0.4156585245735549 1107 41 0.562717124492579 1107 42 0.32984496185817136 1107 43 0.37067723290500254 1107 44 0.1940639381773792 1107 45 0.23674798450867485 1107 46 -0.01974586859216032 1107 47 -0.23608852483268805 1107 48 0.09660236548631573 1107 49 0.14962665554329035 1107 50 -0.2070851433636811 1108 1 0.022199401161039134 1108 2 -0.11587361353564418 1108 3 0.503575426416635 1108 4 0.27985610920672954 1108 5 -0.00734376303347517 1108 6 0.38952432608193277 1108 7 0.3247400247792953 1108 8 0.1154808859566181 1108 9 0.7269433559472276 1108 10 0.4815419477422438 1108 11 0.8685064145993238 1108 12 0.2759406381966265 1108 13 0.5769557665353489 1108 14 0.393753646159702 1108 15 0.5927547151639381 1108 16 0.642191876455037 1108 17 0.7588446439650482 1108 18 0.39397173550662146 1108 19 0.29468340171534635 1108 20 0.5526316543894794 1108 21 0.6478459260115486 1108 22 0.2712548152736325 1108 23 0.2522414702016853 1108 24 0.5293338092604536 1108 25 0.5312312763998435 1108 26 0.4049018608204804 1108 27 0.3234513076691705 1108 28 -0.18803089631087389 1108 29 0.3404156309996722 1108 30 0.4036084701752145 1108 31 0.2814004948069453 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1388.0 6 1365.0 7 2375.0 8 2918.0 9 1892.0 10 1756.0 11 1755.0 12 1979.0 13 1936.0 14 1581.0 15 1409.0 16 1053.0 17 1022.0 18 989.0 19 1051.0 20 1927.0 21 3613.0 22 6631.0 23 11646.0 24 15473.0 25 13768.0 26 9103.0 27 5674.0 28 4048.0 29 3050.0 30 3557.0 31 4633.0 32 6155.0 33 7276.0 34 8636.0 35 16254.0 36 20355.0 37 18023.0 38 20312.0 39 23195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.76510758310359 29.013372627309312 9.364007985549957 11.85751180403714 2 9.147843861164148 46.0466166485767 25.725513187187556 19.080026303071595 3 18.797361801580543 54.32708114638832 17.155023866584802 9.720533185446335 4 7.5329522088973935 37.36762660898297 24.287372578849574 30.812048603270064 5 6.857081728712024 60.36228783340465 19.864023201635526 12.9166072362478 6 16.510138360195995 53.70142242045179 12.009966053624973 17.77847316572723 7 15.054576851177085 59.13310731599125 12.611688476284023 13.200627356547637 8 16.55345054997287 50.24260596434386 13.554246693627286 19.64969679205599 9 19.784611484746943 46.08355711875282 12.162836569310107 21.968994827190127 10 14.941035260003407 51.11888416607576 14.84635892235352 19.093721651567307 11 15.298544410337659 52.67254183582533 14.91553619170215 17.113377562134865 12 23.70474839752478 55.23369595360151 13.58716752105601 7.4743881278177025 13 22.480985580732053 49.09523054983362 13.792188242750752 14.63159562668357 14 18.473270346821955 51.37164022421613 20.542713965971437 9.612375462990473 15 21.101215473115385 50.79710948766303 17.526108108964706 10.575566930256882 16 8.77152670757748 53.59501463835924 25.118552882310762 12.514905771752522 17 15.12679293129408 52.374197638481654 17.562405895871304 14.93660353435296 18 12.308704040440055 51.64188683281634 24.628104412038525 11.421304714705078 19 12.91650325438044 54.4351084859505 23.875624433008884 8.772763826660169 20 13.299233749871235 50.2900181459441 21.926877392055406 14.483870712129256 21 14.448604425427389 50.14044888175749 24.122739248430104 11.288207444385016 22 11.832289401016597 48.80611383814493 18.98253245698484 20.379064303853635 23 11.54824345952113 53.18942431109068 23.80678847037571 11.455543759012471 24 15.679921414227035 50.875177748818636 21.21974309106681 12.225157745887516 25 12.05957281584171 52.075500104993324 22.04027781629734 13.824649262867625 26 20.193043002785494 47.40687616640053 22.60608053760416 9.794000293209816 27 11.085271010708894 48.019270463972866 25.841993288617193 15.053465236701042 28 12.728850634837604 52.953461093410695 25.317815080761484 8.99987319099022 29 12.83725685212409 47.195156260525344 25.636870113762892 14.330716773587671 30 10.559052965623254 55.24723761830997 26.26966550321106 7.924043912855722 31 16.57448932697336 49.2884648907342 20.70503779535046 13.432007986941985 32 10.07103887922598 53.2494443277852 24.39292067655321 12.286596116435614 33 12.819679795246453 51.16223786752033 22.843411872377683 13.174670464855529 34 10.828101170552001 50.57656123115927 23.969973657628394 14.62536394066034 35 12.69829949761486 51.21634257278246 24.094676619280815 11.990681310321875 36 13.799989697951872 49.14433565657726 25.131053939146593 11.924620706324268 37 15.552720111269174 50.1583049678833 24.39362659047951 9.895348330368005 38 16.76406613390809 49.07823785544202 23.759394921492728 10.398301089157162 39 15.225605602116637 47.33638048765031 25.428951662732302 12.009062247500752 40 13.092149305404261 51.258670068013735 24.812932513101657 10.836248113480346 41 11.01112558964833 50.539248497906776 26.194842384179402 12.254783528265493 42 12.800199637958148 53.275211224089645 23.29347175954717 10.631117378405035 43 11.667194677649295 50.38610803104705 23.711389196895297 14.235308094408364 44 12.786954059630212 53.26287110481025 22.679518301303247 11.270656534256286 45 14.270552407202796 48.373747094209016 21.672943571222074 15.682756927366114 46 10.343352738505406 52.44623975288108 25.320185339194488 11.890222169419033 47 14.720544853392465 49.235581777505 20.405868261101983 15.638005108000556 48 13.044201455032217 50.96137471634 23.152200171879144 12.842223656748633 49 14.944335972308581 48.644934395260144 23.151562196778574 13.2591674356527 50 11.794827115073126 51.448463223093036 24.414179296579526 12.342530365254307 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 28804.0 1 25057.0 2 21310.0 3 20843.0 4 20376.0 5 14005.0 6 7634.0 7 5353.0 8 3072.0 9 2616.0 10 2160.0 11 2456.0 12 2752.0 13 4416.5 14 6081.0 15 7768.0 16 9455.0 17 8319.5 18 7184.0 19 5605.5 20 4027.0 21 3597.0 22 3167.0 23 3011.5 24 2856.0 25 2886.5 26 2917.0 27 2883.0 28 2849.0 29 2903.0 30 2957.0 31 2947.0 32 2937.0 33 3167.5 34 3398.0 35 3360.5 36 3323.0 37 3131.0 38 2939.0 39 2941.0 40 2943.0 41 3065.0 42 3187.0 43 3966.5 44 4746.0 45 8043.0 46 11340.0 47 26469.5 48 41599.0 49 32692.0 50 23785.0 51 17364.5 52 10944.0 53 6848.5 54 2753.0 55 2367.5 56 1982.0 57 1886.0 58 1790.0 59 1626.5 60 1463.0 61 1276.5 62 1090.0 63 1003.0 64 916.0 65 764.0 66 612.0 67 569.5 68 527.0 69 508.0 70 489.0 71 477.5 72 466.0 73 468.0 74 470.0 75 427.0 76 384.0 77 334.0 78 284.0 79 253.0 80 222.0 81 188.0 82 154.0 83 123.0 84 92.0 85 83.5 86 75.0 87 50.5 88 26.0 89 23.5 90 21.0 91 17.0 92 13.0 93 15.0 94 17.0 95 13.5 96 10.0 97 8.5 98 7.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061754785995914684 2 0.06729688217503524 3 0.06610929013665226 4 0.0795686665716593 5 0.08550662676357418 6 0.06135892198312036 7 0.04908713758649629 8 0.05740028185517711 9 0.05462923376561683 10 0.06848447421341822 11 0.0542333697528225 12 0.07521416243092173 13 0.06808861020062389 14 0.0700679302645955 15 0.07917280255886498 16 0.07640175446930471 17 0.09184045096828337 18 0.0748182984181274 19 0.07244311434136146 20 0.08471489873798552 21 0.08194385064842526 22 0.0795686665716593 23 0.07283897835415577 24 0.05898373790635441 25 0.08511076275077985 26 0.0922363149810777 27 0.08115212262283661 28 0.10450809937770178 29 0.0969866831346096 30 0.08036039459724796 31 0.07917280255886498 32 0.08511076275077985 33 0.08352730669960255 34 0.06610929013665226 35 0.08550662676357418 36 0.09302804300666635 37 0.09936186721137555 38 0.08511076275077985 39 0.05383750574002818 40 0.06452583408547496 41 0.051858185676056566 42 0.06135892198312036 43 0.036419489177077886 44 0.035231897138694915 45 0.03919053726663817 46 0.04037812930502114 47 0.02652288885721977 48 0.04314917739458141 49 0.04552436147134736 50 0.04077399331781546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 252612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.4268997513974 #Duplication Level Percentage of deduplicated Percentage of total 1 89.79990112149046 27.323325891089894 2 5.016783325960813 3.0529032666698335 3 1.7134604876271762 1.5640587145503777 4 0.9016158830111108 1.0973350434658686 5 0.5204132080872212 0.7917280255886499 6 0.3629882126408368 0.6626763574176998 7 0.23288491061903152 0.4960176080312891 8 0.1639301605474747 0.3990309248966795 9 0.14311363222398585 0.39190537266638165 >10 0.8131456376362832 4.673966399062595 >50 0.11318987275897062 2.429417446518772 >100 0.15352189638573027 10.948806865865437 >500 0.03122479248523328 6.582822668756829 >1k 0.027321693424579116 18.436574667870094 >5k 0.005204132080872212 10.302756796985099 >10k+ 0.001301033020218053 10.846673950564503 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27400 10.846673950564503 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7252 2.8708058207844442 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7024 2.780548825867338 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6568 2.600034836033126 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5182 2.0513673143001916 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3890 1.5399110097699238 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3769 1.4920114642218105 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3740 1.480531407850775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3594 1.4227352619828038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 1.3103098823492154 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3180 1.258847560685953 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2966 1.1741326619479675 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2412 0.9548239988599115 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2330 0.9223631498107769 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.9049451332478267 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2114 0.8368565230472028 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1775 0.7026586227099267 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1656 0.655550805187402 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1396 0.5526261618608775 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1340 0.5304577771443953 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1282 0.5074976644023246 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1182 0.467911263122892 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1180 0.4671195350973034 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1069 0.42317862967713327 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1064 0.4211993096131617 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1038 0.41090684528050925 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 985 0.38992605260241 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 914 0.36181970769401295 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 909 0.35984038763004134 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 861 0.34083891501591373 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.33648441087517617 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 833 0.32975472265767264 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 773 0.3060028818900132 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.29887732965971525 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 724 0.28660554526309123 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 718 0.2842303611863253 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 706 0.27947999303279336 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 688 0.27235444080249555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 681 0.26958339271293524 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 647 0.2561240162779282 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 641 0.2537488322011623 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 632 0.25018605608601335 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 593 0.23474735958703466 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 546 0.2161417509857014 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 542 0.21455829493452408 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 541 0.21416243092172976 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 538 0.21297483888334678 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 534 0.2113913828321695 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 511 0.20228651053790003 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 507 0.2007030544867227 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 471 0.18645195002612702 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 466 0.1844726299621554 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 462 0.1828891739109781 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 459 0.18170158187259514 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 457 0.18090985384700647 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 450 0.1781388057574462 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.17378430161670863 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 436 0.17259670957832565 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 435 0.17220084556553134 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 403 0.15953319715611292 No Hit GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 401 0.15874146913052428 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 394 0.155970421040964 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 387 0.15319937295140373 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 386 0.15280350893860942 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 383 0.15161591690022644 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 378 0.1496365968362548 No Hit AACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 371 0.14686554874669455 No Hit GCAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 366 0.1448862286827229 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 360 0.14251104460595695 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 358 0.1417193165803683 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 345 0.1365730844140421 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 340 0.13459376435007048 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 339 0.13419790033727613 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 332 0.13142685224771586 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 331 0.13103098823492154 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 325 0.12865580415815558 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 322 0.1274682121197726 No Hit GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 320 0.12667648409418397 No Hit GAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 0.125488892055801 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 0.125488892055801 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 313 0.12390543600462368 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 306 0.12113438791506341 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.11717574778712017 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 294 0.11638401976153151 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 293 0.11598815574873719 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 288 0.11400883568476557 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 281 0.11123778759520529 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 280 0.11084192358241096 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 278 0.11005019555682231 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 278 0.11005019555682231 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 278 0.11005019555682231 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 266 0.10529982740329043 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 263 0.10411223536490745 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.10213291530093582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 3.958640127943249E-4 0.0 15 0.0 0.0 0.0 3.958640127943249E-4 0.0 16 0.0 0.0 0.0 3.958640127943249E-4 0.0 17 0.0 0.0 0.0 0.0015834560511772996 0.0 18 0.0 0.0 0.0 0.0023751840767659493 0.0 19 0.0 0.0 0.0 0.003166912102354599 0.0 20 0.0 0.0 0.0 0.003166912102354599 0.0 21 0.0 0.0 0.0 0.006729688217503523 0.0 22 0.0 0.0 0.0 0.011084192358241096 0.0 23 0.0 0.0 0.0 0.020584928665304893 0.0 24 0.0 0.0 0.0 0.03325257707472329 0.0 25 0.0 0.0 0.0 0.03641948917707789 0.0 26 0.0 0.0 0.0 0.04750368153531899 0.0 27 0.0 0.0 0.0 0.052649913701645214 0.0 28 0.0 0.0 0.0 0.0684844742134182 0.0 29 0.0 0.0 0.0 0.0906528589299004 0.0 30 0.0 0.0 0.0 0.12469716403021235 0.0 31 0.0 0.0 0.0 0.15992906116890726 0.0 32 0.0 0.0 0.0 0.20426583060187165 0.0 33 0.0 0.0 0.0 0.2588950643674885 0.0 34 0.0 0.0 0.0 0.31748293826104856 0.0 35 0.0 0.0 0.0 0.39190537266638165 0.0 36 0.0 0.0 0.0 0.4611815749053885 0.0 37 0.0 0.0 0.0 0.5344164172723386 0.0 38 0.0 0.0 0.0 0.6258610042278276 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTTT 20 7.8437553E-4 44.00119 15 GTACGTT 20 7.8437553E-4 44.00119 14 GAACAAA 915 0.0 38.470985 1 GAGGCTG 25 0.0023489688 35.20095 9 TAACAAC 145 0.0 34.897495 1 GAAAACA 580 0.0 34.13885 1 GTACATA 85 0.0 33.64797 1 TACAAGG 35 3.2102014E-4 31.42942 2 GTAACAA 35 3.2102014E-4 31.42942 1 ACAAGAG 50 2.3512002E-6 30.80083 3 GTACAAA 90 1.6370905E-11 29.334126 1 TACAAGA 60 2.9291004E-7 29.334124 2 AGGCTGA 30 0.005735762 29.334124 10 AATACAA 30 0.005735762 29.334124 2 GAATACA 55 4.939635E-6 28.000755 1 AGAACAA 225 0.0 27.37852 2 CAAGAGA 50 8.3142666E-5 26.400713 4 GAGAACA 385 0.0 26.286423 1 GAAACAA 440 0.0 25.500689 1 GTATAAA 45 0.0013949359 24.445105 1 >>END_MODULE